A5087450674- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Polysaccharides and Plant Cell Walls
- Microbial Community Ecology and Physiology
- Botanical Research and Applications
- Protist diversity and phylogeny
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Polysaccharides Composition and Applications
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
University of Wisconsin–Madison
2016-2021
Significance Bacterial promoters are often predicted by similarity to the Escherichia coli −10 and −35 consensus elements. Although these elements highly conserved in diverse bacterial phyla, only 30 43% of we analyzed from Rhodobacter sphaeroides two other α-proteobacteria contained −7T, a base element present 90 99% non–α-proteobacteria. Expression 15 16 R. tested vitro that lacked −7T was very weak, but identified an essential transcription factor, CarD, activated all promoters. We...
The multi-subunit bacterial RNA polymerase (RNAP) and its associated regulators carry out transcription integrate myriad regulatory signals. Numerous studies have interrogated RNAP mechanism, mutations drive Escherichia coli adaptation to many health- industry-relevant environments, yet a paucity of systematic analyses hampers our understanding the fitness trade-offs from altering function. Here, we conduct chemical-genetic analysis library mutants. We discover phenotypes for non-essential...
Bioinformatic analysis showed previously that a majority of promoters in the photoheterotrophic alphaproteobacterium Rhodobacter sphaeroides lack thymine at last position -10 element (-7T), base is very highly conserved bacteria other than alphaproteobacteria. The absence -7T was correlated with low promoter activity using purified R. RNA polymerase (RNAP), but transcription factor CarD compensated by activating almost all lacking tested vitro, including rRNA promoters. Here, we show...