- Microbial bioremediation and biosurfactants
- Oil Spill Detection and Mitigation
- Toxic Organic Pollutants Impact
- Atmospheric and Environmental Gas Dynamics
- Hydrocarbon exploration and reservoir analysis
- Petroleum Processing and Analysis
- Antimicrobial Resistance in Staphylococcus
- Methane Hydrates and Related Phenomena
- Carbon Dioxide Capture Technologies
- Microbial Community Ecology and Physiology
- Environmental Toxicology and Ecotoxicology
- Environmental Chemistry and Analysis
- Marine and coastal ecosystems
- Bacteriophages and microbial interactions
- Drilling and Well Engineering
- Bacterial Identification and Susceptibility Testing
- Reproductive biology and impacts on aquatic species
- Microplastics and Plastic Pollution
- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Enhanced Oil Recovery Techniques
- Bacterial Infections and Vaccines
- biodegradable polymer synthesis and properties
- Water Quality Monitoring and Analysis
- Ocean Acidification Effects and Responses
SINTEF
2016-2025
SINTEF Ocean
2004
Norwegian University of Science and Technology
1989-2000
Synthetic oligonucleotide primers of 21 and 24 bases, respectively, were used in the polymerase chain reaction (PCR) to amplify a sequence nuc gene, which encodes thermostable nuclease Staphylococcus aureus. A DNA fragment approximately 270 bp was amplified from lysed S. aureus cells or isolated DNA. The PCR product detected by agarose gel electrophoresis Southern blot analysis using 33-mer internal gene hybridization probe. With lower detection limit less than 10 CFU, with target 0.69 pg...
The process of post combustion CO2-capture by chemical absorption relies on large scale use chemicals, typically amines in aqueous solution. In such operations, emissions may occur through the cleaned exhaust gas, as degraded solvent and accidental spills. It is thus important that chemicals used have low or no environmental effects. To check this, standard ecotoxicity biodegradability tests for a marine environment were performed more than 40 amines, including both solvents already...
Chemical and toxicological characterization of unresolved complex mixtures in the water-soluble fraction an artificially weathered Norwegian Sea crude oil was determined by a combination chemical analysis toxicity testing fish vitro bioassays. The separated into 14 increasingly polar fractions preparative high-pressure liquid chromatography. (7-ethoxyresorufin O-deethylase activity, estrogenicity, metabolic inhibition) these characterized primary culture liver cells (hepatocytes) from...
Summary The D eepwater H orizon ( DWH ) accident in 2010 created a deepwater plume of small oil droplets from well the M ississippi C anyon lease block 252 (‘ acondo oil’). A novel laboratory system was used current study to investigate biodegradation dispersions (10 μm or 30 median droplet sizes) at low concentrations (2 mg l −1 coastal N orwegian seawater temperature 4–5°C. Whole metagenome analyses showed that associated with successive increased abundances Gammaproteobacteria, while...
This study investigates a comparative multivariate approach for studying the biodegradation of chemically dispersed oil. The rationale this lies in inherent complexity data and challenges associated with comparing multiple experiments inconsistent sampling points, respect to inferring correlations visualizing datasets numerous variables. We aim identify novel among microbial community composition, chemical change individual petroleum hydrocarbons, oil type temperature by creating modelled...
A multiplex polymerase chain reaction (mPCR) was used for simultaneous amplification of the staphylococcal nuc gene, encoding thermostable nuclease (TNase), and mecA penicillin-binding protein 2a which is associated with methicillin resistance. total 219 strains were tested mPCR data compared coagulase production in vitro oxacillin susceptibility. The agreement 100% amplification, 97.7%, 96.8 97.3% resistance MIC determination, disk diffusion agar screen methods, respectively. Discrepant...
Seasonal dynamics can vastly influence the natural depletion of oil spilled into ocean and Arctic regions are characterized by large seasonal changes, especially in temperature daylight. To determine influences variation on processes like dissolution, photooxidation biodegradation, we deployed thin films three oils seawater during summer winter Svalbard, Norway. The extent varied with season type oil, however, considerable n-alkanes polycyclic aromatic compounds were observed both winter....