A5052300536- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Insect-Plant Interactions and Control
- Horticultural and Viticultural Research
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogens and Fungal Diseases
- Transgenic Plants and Applications
- Smart Agriculture and AI
- Helminth infection and control
- Plant Pathogenic Bacteria Studies
- Parasite Biology and Host Interactions
- Genetic Mapping and Diversity in Plants and Animals
- Vector-Borne Animal Diseases
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
Cornell University
2019-2025
Louisiana State University
2025
Louisiana State University Agricultural Center
2024-2025
Plant (United States)
2022-2023
Cornell University Agricultural Experiment Station
2019
The epidemiological relationship between arthropod dispersal and plant communities is poorly understood at the landscape level. We investigated connectivity of natural cultivated plants in vineyard ecosystems for red blotch disease dynamics by characterizing dietary history Spissistilus festinus, a treehopper vector grapevine virus (GRBV). Molecular analysis gut content 205 S. festinus caught 71 sites flora proximal to vineyards Napa Valley, California, USA revealed total 171 genera from 61...
Limited information is available on the spread dynamics of grapevine red blotch virus (GRBV, genus Grablovirus, family Geminiviridae) in vineyards. In this study, we investigated disease progress three vineyards with a disparate initial inoculum prevalence. Secondary was documented Cabernet Sauvignon and franc California, but not Merlot vineyard New York. Increase annual incidence (4.8, 0.13, 0%) unrelated to estimated source at planting (1, 40, 40%) franc, Sauvignon, vineyards,...
Introduction Grapevine leafroll-associated viruses (GLRaVs) and grapevine red blotch virus (GRBV) cause substantial economic losses concern to North America’s grape wine industries. Fast accurate identification of these two groups is key informing disease management strategies limiting their spread by insect vectors in the vineyard. Hyperspectral imaging offers new opportunities for scouting. Methods Here we used machine learning methods, i.e., Random Forest (RF) 3D-Convolutional Neural...
Spissistilus festinus (Hemiptera: Membracidae) transmit grapevine red blotch virus (GRBV, Grablovirus, Geminiviridae) in greenhouse settings; however, their role as a vector of GRBV vineyards is unknown. Following controlled exposures aviruliferous S. for two weeks on infected, asymptomatic vines California vineyard June and 48 h gut clearing alfalfa, nonhost GRBV, approximately half the released insects tested positive (45%, 46 102), including salivary glands dissected individuals (11%, 3...
The transmission mode of grapevine red blotch virus (GRBV, genus Grablovirus, family Geminiviridae) by Spissistilus festinus, the three-cornered alfalfa hopper, is unknown. By analogy with other members in Geminiviridae, we hypothesized circulative, nonpropagative transmission. Time-course experiments revealed GRBV dissected guts, hemolymph, and heads salivary glands after a 5-, 8-, 10-day exposure to infected grapevines, respectively. After 15-day acquisition on grapevines subsequent...
Red blotch disease, a threat to the grape industry, is caused by grapevine red virus. This work first study epidemiological patterns in vineyard over course of decade, revealing an increase disease incidence from 3.9% 2014 36.4% 2023 with rapid virus spread proximal transmission hotspot. Logistic and exponential models provided best fit areas high low aggregation, respectively. An inverse spatial strains 1 2 suggested secondary mostly diseased neighboring vines influx background sources....
Grapevine red blotch disease emerged within the past decade, disrupting North American vine stock production and vineyard profitability. Our understanding of how grapevine virus (GRBV), causal agent disease, interacts with its Vitis hosts insect vector, Spissistilus festinus, is limited. Here, we studied capabilities S. festinus to transmit GRBV from free-living vines, identified as first-generation hybrids V. californica vinifera ‘Sauvignon blanc’ (Vcal hybrids), ‘Cabernet franc’ (Vvin Cf)...
The mechanisms underlying host plant symptom development upon infection by viruses of the genus Nepovirus in family Secoviridae, including grapevine fanleaf virus (GFLV), are poorly understood. In systemic Nicotiana benthamiana, GFLV strain GHu produces characteristic symptoms vein clearing apical leaves, unlike other strains such as F13, which cause an asymptomatic infection. this study, we expanded on earlier findings and used reverse genetics to identify residue 802 (lysine, K) GFLV-GHu...
Grapevine red blotch virus (GRBV) causes disease and is transmitted by the three-cornered alfalfa hopper,
Grapevine red blotch virus (GRBV) is the causative agent of disease. Limited information available on seasonal variation GRBV titer in relation to disease symptom expression vineyards across United States. In this study, no statistically significant difference was found among asymptomatic infected vines June ( P = 0.451) and symptomatic October 0.068) a diseased ‘Cabernet franc’ vineyard California, regardless years symptomatic, one seven, as shown by qPCR. Similarly, there were differences...
<h3>Abstract</h3> <h3>Background and goals</h3> Grapevine red blotch virus (GRBV) is the causal agent of disease, a major threat to grape production vineyard profitability. Several species <i>Cicadellidae</i> <i>Membracidae</i>, including three-cornered alfalfa hopper (<i>Spissistilus festinus</i>), have been reported transmit GRBV in greenhouse, but their capacity act as vectors remains source confusion. This article examines literature on transmission provides recommendations convincingly...
A decade since its discovery, Grapevine Red Blotch Virus (GRBV) has revealed significant impact on grapevine physiology, grape composition, and wine production. To mitigate spread in vineyards, the imperative to identify remove diseased vines necessitates autonomous scouting for enhanced roguing as a disease management response. Remote sensing, especially hyperspectral imagery, shows promise identifying virus-infected vines. However, detailed data it provides, while rich, presents processing...
Abstract Sugarcane yellow leaf virus (SCYLV) is the causal agent of disease, a significant threat to global sugarcane production. SCYLV disseminated via infected planting material and transmitted by aphids, primarily Melanaphis sacchari (Zehntner). The disease’s impact varies depending on cultivar, with some varieties showing little no foliar symptoms, yet experiencing severe yield losses. Reliable diagnostic methods, including serological molecular techniques, are crucial for accurate...
Two phenotypically similar but genetically distinct genotypes of Spissistilus festinus (Say, 1830) (Hemiptera: Membracidae), a pest legume crops in Southern United States and vector grapevine red blotch virus (GRBV) California vineyards, exist. No information is available on whether the two S. genotypes, i.e., (CA) Southeastern (SE), are sexually compatible or SE genotype can transmit GRBV. In this study, we established mixed mating pairs for which F1 offspring varied compared with same...