A5033460972- Bacillus and Francisella bacterial research
- SARS-CoV-2 and COVID-19 Research
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Milk Quality and Mastitis in Dairy Cows
- Microbial infections and disease research
- Monoclonal and Polyclonal Antibodies Research
- Yersinia bacterium, plague, ectoparasites research
- Microbial Metabolism and Applications
- Pneumonia and Respiratory Infections
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Streptococcal Infections and Treatments
- Antimicrobial Peptides and Activities
- Poxvirus research and outbreaks
- Vaccine Coverage and Hesitancy
- Plant Pathogenic Bacteria Studies
- Glycosylation and Glycoproteins Research
- Immunodeficiency and Autoimmune Disorders
- Vehicle License Plate Recognition
- vaccines and immunoinformatics approaches
- Probiotics and Fermented Foods
- Traffic Prediction and Management Techniques
Dr. Reddy's Foundation
2023
Bharat Biotech (India)
2022
Visvesvaraya Technological University
2020
BLDE (Deemed to be University)
2020
Albany Medical Center Hospital
2015-2018
Karnataka Veterinary Animal and Fisheries Sciences University
2014
Indian Institute of Science Bangalore
2011
Gulbarga University
2010
Most if not all vaccine candidates developed to combat COVID-19 due SARS-CoV-2 infection are administered parenterally. As is transmitted through infectious respiratory fluids, vaccine-induced mucosal immunity could provide an important contribution control this pandemic. ChAd-SARS-CoV-2-S (BBV154), a replication-defective chimpanzee adenovirus (ChAd)-vectored intranasal (IN) candidate, encodes prefusion-stabilized version of the spike protein containing two proline substitutions in S2...
Francisella tularensis (Ft) is a gram-negative intercellular pathogen and category A biothreat agent. However, despite 15 years of strong government investment intense research focused on the development US Food Drug Administration-approved vaccine against Ft, primary goal remains elusive. This article reviews efforts developing an Ft vaccine, as well number important factors, some only recently recognized such, which can significantly impact evaluation efficacy. Finally, assessment provided...
The gram-negative bacterium Francisella tularensis (Ft) is both a potential biological weapon and naturally occurring microbe that survives in arthropods, fresh water amoeba, mammals with distinct phenotypes various environments. Previously, we used number of measurements to characterize Ft grown Brain-Heart Infusion (BHI) broth as 1) more similar infection-derived bacteria, 2) slightly virulent naïve animals, compared Mueller Hinton Broth (MHB). In these studies observed the free amino...
Francisella tularensis (Ft) is a biothreat agent for which there no FDA-approved human vaccine. Currently, are substantial efforts underway to develop both vaccines and the tools assess these vaccines. Tularemia laboratory research has historically relied primarily upon small number of inbred mouse strains, but utility such findings outbred animals may be limited. Specifically, C57BL/6 mice more susceptible than BALB/c Ft infection less easily protected against challenge with highly virulent...
Abstract The development of persistent antibiotic resistance by human methicillin‐sensitive Staphylococcus aureus (MSSA) strains and substantial association with poly‐ N ‐acetyl glucosamine (PNAG) in biofilms is reported this investigation. Sixteen 31 MSSA under study were found to have developed one or more antibiotics, four strains, two which did not produce biofilms, showing cefoxitin, undetectable mecA amplification. Antibiotic displayed 13/14 biofilm‐forming S. isolates remained for 4...
To determine the prevalence of different capsular polysaccharide (CP) and major surface-associated non-CP antigen 336 (SP-336) types among Staphylococcus aureus isolated from bovine mastitis cases in Australia India.A total 414 strains (154 Australia, 260 India) clinical were included study. Mouse antisera raised against CP (CP1, CP2, CP5, CP8) or SP-336 used slide agglutination tests compared with detection cap1, cap5 cap8 gene fragments by PCR.Serological studies revealed presence CP8...
Tularemia is caused by a gram-negative, intracellular bacterial pathogen, Francisella tularensis (Ft). The history weaponization of Ft in the past has elevated concerns that it could be used as bioweapon or an agent bioterrorism. Since discovery Ft, three broad approaches adopted for tularemia vaccine development have included inactivated, live attenuated, subunit vaccines. Shortcomings each these hampered suitable prevention tularemia. Recently, we reported oxidant sensitive mutant LVS...
The problem of traffic congestion needs to be addressed in an efficient way, specially the country like India where this is a very big due huge population. present control systems that are used either manually controlled or automatic controlling system with static time allocation, both have certain drawbacks, one requires more man power operate and less other uses allocation which results wastage time. In paper image processing technique by dynamically allocating release vehicles depending...
Fc gamma receptor IIB (Fc γ RIIB) is the only R) which negatively regulates immune response, when engaged by antigen- (Ag-) antibody (Ab) complexes. Thus, generation of Ag-specific IgG in response to infection or immunization has potential downmodulate protection against infection. Therefore, we sought determine impact RIIB on Francisella tularensis ( Ft ), a Category A biothreat agent. We utilized inactivated (i ) as an immunogen. Naïve and i -immunized knockout (KO) wildtype (WT) mice were...
Francisella tularensis (Ft) is a Category A biothreat agent for which there no FDA-approved vaccine. Ft can survive in variety of habitats with remarkable ability to adapt changing environmental conditions. Furthermore, expresses distinct sets antigens (Ags) when inside macrophages (its vivo host) as compared those grown vitro Mueller Hinton Broth medium (MHB). However, contrast MHB-grown Ft, Brain Heart Infusion (BHI) more closely mimics the antigenic profile macrophage-grown Ft. Thus, we...
The aim of this study was to compare the performance serological versus molecular typing methods detect capsular polysaccharide (CP) and surface-associated antigen 336 phenotypes Staphylococcus aureus isolates. Molecular CP types 1, 5 8 carried out using PCR, whereas CP1, 2, 5, by slide agglutination specific antisera. By genotyping, 14/31 strains were CP8 positive, 12/31 CP5 remaining 6/31 isolates non-typable (NT). One isolate positive for both but confirmed as type serologically....
Lack of safe and effective mucosal adjuvants has severely hampered the development subunit vaccines. In this regard, we have previously shown that immunogenicity vaccine antigens can be improved by targeting to antigen-presenting cells. Specifically, groups mice immunized intranasally with a fusion protein (Bivalent-FP) containing fragment pneumococcal-surface-protein-A (PspA) as antigen single-chain bivalent antibody raised against anti-human Fc-gamma-receptor-I (hFcγRI) elicited protective...
Staphylococci are broadly classified as coagulase-positive or-negative based on their ability to coagulate plasma. Besides the Staphylococcus aureus, coagulase-negative staphylococci also gaining significance in bovine mastitis. The precise grouping of or-positive is an important preliminary step establishing aetiology While investigating distribution various species among 149 isolates obtained from milk samples that were standard tube coagulase test, 19 identified S. aureus by a...
Subclinical mastitis (SCM) is an important condition affecting quality milk production. Several coagulase negative staphylococci (CoNS) have recently been identified to be associated with SCM throughout the world. Studies on comparative evaluation of tests detect or contribution CoNS in India are lacking. This study evaluated 313 samples from organized and unorganized sectors Somatic Cell Count (SCC), Electrical Conductivity (EC), California Mastitis Test (CMT) Bromothymol Blue (BTB) SCM,...
Francisella tularensis (Ft) is a biothreat agent for which there no FDA-approved human vaccine. Currently, are substantial efforts underway to develop both vaccines and improved tools assess these vaccines. Ft expresses distinct sets of antigens (Ags) in vivo as compared those expressed vitro. Importantly, grown Brain-Heart Infusion Medium (BHIM) more closely mimics the antigenic profile macrophage-grown when Muller Hinton (MHM)-grown Ft. Thus, we predicted that used live vaccine BHIM-grown...
Biofilm formation is an important virulence determinant of Staphylococcus aureus which a major etiological agent bovine mastitis. Here, 132 mastitis-associated S. were subjected to biofilm production, antimicrobial susceptibility, and the detection ica, bap, agr blaZ genes. It was found that 33.3% isolates produced biofilm. The number resistant individual antibiotics increased by 1.2- 7.0-fold when growing in versus planktonic mode growth, spectrum as well various with increase density...
Abstract Francisella tularensis (Ft) is a Category A biothreat agent for which there currently no FDA-approved vaccine. Thus, substantial effort underway to develop an effective tularemia While it well established that gender can significantly impact susceptibility primary infection, the of on vaccine efficacy not established. development successful against will require understanding this impact. In study, role in vaccine-induced protection identified first time. Mucosal vaccination with...