A5067134168- SARS-CoV-2 and COVID-19 Research
- Bacillus and Francisella bacterial research
- Research on Leishmaniasis Studies
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Amoebic Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Trypanosoma species research and implications
- Parasitic Infections and Diagnostics
- Biofuel production and bioconversion
- Immunotherapy and Immune Responses
- Pneumonia and Respiratory Infections
- Clostridium difficile and Clostridium perfringens research
- Respiratory viral infections research
- Cytokine Signaling Pathways and Interactions
- Cancer Research and Treatments
- COVID-19 Clinical Research Studies
- Immune Response and Inflammation
- Enzyme Production and Characterization
- Ear and Head Tumors
- Yersinia bacterium, plague, ectoparasites research
- Fungal and yeast genetics research
- Poxvirus research and outbreaks
- vaccines and immunoinformatics approaches
- Glycosylation and Glycoproteins Research
Banaras Hindu University
2025
Institute of Medical Sciences
2025
Albany Medical Center Hospital
2015-2025
University of New Mexico
2011-2022
Maharashtra Institute of Medical Education and Research
2021
Maharashtra Institute of Medical Science and Research
2021
Curtin University
2017
New Mexico VA Health Care System
2012
Rajendra Memorial Research Institute of Medical Sciences
2009-2011
Queen Victoria Hospital
2005
ABSTRACT The clinical value of amphotericin B, the mainstay therapy for visceral leishmaniasis in sodium antimony gluconate-nonresponsive zones Bihar, India, is now threatened by emergence acquired drug resistance, and a comprehensive understanding underlying mechanisms need hour. We have selected an B-resistant isolate which demonstrated 8-fold-higher 50% lethal doses (LD 50 ) than B-sensitive strain to explore mechanism B resistance. Fluorimetric analysis lower anisotropy motion...
This study investigated molecular signals essential to sustain cancer stem cells (CSCs) and assessed their activity in the presence of secreted frizzled-related protein 4 (sFRP4) alone or combination with chemotherapeutic drugs. SFRP4 is a known Wnt antagonist, also pro-apoptotic anti-angiogenic. Additionally, sFRP4 has been demonstrated confer chemo-sensitization improve efficacy. CSCs were isolated from breast, prostate, ovary tumor cell lines, characterized using tumor-specific markers...
Expression of the Aspergillus nidulans 22 kDa endoxylanase gene, xlnA , is controlled by at least three mechanisms: specific induction xylan or xylose; carbon catabolite repression (CCR); and regulation ambient pH. Deletion analysis upstream sequences has identified two positively acting regions: one that mediates another influence pH contains PacC consensus binding sites. The extreme derepressed mutation creA d 30 results in considerable, although not total, loss glucose repressibility,...
β-xylosidase (EC 3.2.1.37) has been purified from Aspergillus nidulans mycelium grown on oat-spelt xylan as sole carbon source. Its pH optimum for activity was found to be 5.0 and the temperature 50 °C. molecular mass estimated by gel filtration 180000. Using p-nitrophenyl-β-d-xylopyranoside substrate, Km Vmax values have 1.1 mM 25.6 µmol min−1(mg protein)−1, respectively. Enzyme inhibited Hg2+, Ag2+, Cu2+ at a concentration of 1 × 10−3 M. The synthesis in A. is strongly induced arabinose...
Visceral leishmaniasis (VL), caused by Leishmania donovani , is a major health concern in India. It represents T‐helper type 2 (Th2) bias of cytokines active state and Th1 at cure. However, the role parasite regulating Toll‐like receptor (TLR)‐mediated macrophage activation VL patients remains elusive. In this report, we demonstrated that later stages L. infection rendered tolerance to macrophages, leading incapability for production inflammatory like tumor necrosis factor (TNF)‐α...
Francisella tularensis (Ft) is a gram-negative intercellular pathogen and category A biothreat agent. However, despite 15 years of strong government investment intense research focused on the development US Food Drug Administration-approved vaccine against Ft, primary goal remains elusive. This article reviews efforts developing an Ft vaccine, as well number important factors, some only recently recognized such, which can significantly impact evaluation efficacy. Finally, assessment provided...
Streptococcus pneumoniae (Spn) is a common pathogen causing secondary bacterial infection following influenza, which leads to severe morbidity and mortality during seasonal pandemic influenza. Therefore, there an urgent need develop vaccines that prevent post-influenza pneumonia. Here, improved Yersinia pseudotuberculosis strain (designated as YptbS46) possessing Asd+ plasmid pSMV92 could synthesize high amounts of the Spn pneumococcal surface protein A (PspA) antigen monophosphoryl lipid...
ABSTRACT The head and neck regions, rich in lymph nodes, are common initial sites for nodal extranodal hematolymphoid neoplasms, particularly B-cell neoplasms. Lymphomas, including Hodgkin’s non-Hodgkin’s types, range from indolent to aggressive often affect the oral maxillofacial areas. Plasma cell such as solitary bone plasmacytoma (SBP) extramedullary (SEP), must be distinguished multiple myeloma (MM) due varied treatment modalities. Diagnosing these neoplasms is challenging, requiring...
ABSTRACT The Aspergillus nidulans xlnB gene, which encodes the acidic endo-β-(1,4)-xylanase X 24 , is expressed when xylose present as sole carbon source and repressed in presence of glucose. That mutation creA d 30 results considerably elevated levels mRNA indicates a role for wide-domain repressor CreA repression promoter ( xlnBp ) activity. Functional analyses :: goxC reporter constructs show that none four consensus target sites identified are functional vivo. thus likely to exert...
Programmed cell death (PCD) is an essential process in the growth and development of multicellular organisms. However, accumulating evidence indicates that unicellular eukaryotes can also undergo PCD with apoptosis-like features. This study demonstrates after exposure to 0.8 mM H(2)O(2) for 9 h Entamoeba histolytica presents morphological biochemical death. Morphological characteristics including DNA fragmentation, increased vacuolization, nuclear condensation rounding were observed...
The gram-negative bacterium Francisella tularensis (Ft) is both a potential biological weapon and naturally occurring microbe that survives in arthropods, fresh water amoeba, mammals with distinct phenotypes various environments. Previously, we used number of measurements to characterize Ft grown Brain-Heart Infusion (BHI) broth as 1) more similar infection-derived bacteria, 2) slightly virulent naïve animals, compared Mueller Hinton Broth (MHB). In these studies observed the free amino...
Monitoring the antibody responses to SARS-CoV-2 infection and its correlation clinical spectrum of disease is critical in understanding progression protection against re-infection. We assessed nucleocapsid (N) receptor-binding-domain spike (SRBD) protein specific IgG neutralizing (NAb) COVID-19 patients up 8 months with diverse spectrum.During first wave pandemic, from 284 patients, 608 samples were collected post infection. The categorized as asymptomatic, symptomatic severe. N SRBD NAb...
Visceral leishmaniasis, which is caused by Leishmania donovani , one of the major health problems Indian subcontinent. Infected hosts have been reported to impaired lymphoproliferation. However, fate anergic cells still elusive. In present investigation, L. donovani‐ infected hamsters were used study mechanism lymphocyte cell death. Lymph node‐derived lymphocytes analysed for apoptotic death through mitochondrial abnormality, caspase activity and DNA degradation. The data demonstrate that...
Francisella tularensis (Ft) is a biothreat agent for which there no FDA-approved human vaccine. Currently, are substantial efforts underway to develop both vaccines and the tools assess these vaccines. Tularemia laboratory research has historically relied primarily upon small number of inbred mouse strains, but utility such findings outbred animals may be limited. Specifically, C57BL/6 mice more susceptible than BALB/c Ft infection less easily protected against challenge with highly virulent...