A5060094900- Virology and Viral Diseases
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Vaccine Coverage and Hesitancy
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- COVID-19 epidemiological studies
- Syphilis Diagnosis and Treatment
- Biosimilars and Bioanalytical Methods
- Blood groups and transfusion
- Parvovirus B19 Infection Studies
- Clinical Laboratory Practices and Quality Control
ICMR-National Institute of Virology
2018-2024
Brihanmumbai Municipal Corporation
2020-2022
Indian Council of Medical Research
2022
The global pandemic caused by SARS-CoV-2 virus has challenged public health system worldwide due to the unavailability of approved preventive and therapeutic options. Identification neutralizing antibodies (NAb) understanding their role is important. However, data on kinetics NAb response among COVID-19 patients are unclear. To understand in patients, we compared findings microneutralization test (MNT) plaque reduction neutralization (PRNT) for SARS-CoV-2. Further, was assessed.A total 343...
Background & objectives: Since the beginning of year 2020, pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) impacted humankind adversely in almost all spheres life. The virus belongs to genus Betacoronavirus family Coronaviridae . SARS-CoV-2 causes disease known as 2019 (COVID-19) with mild-to-severe illness. currently available diagnostic tools for diagnosis COVID-19 are mainly based on molecular assays. Real-time reverse transcription-polymerase chain...
Abstract The pandemic of COVID‐19 has caused enormous fatalities worldwide. Serological assays are important for detection asymptomatic or mild cases COVID‐19, and sero‐prevalence vaccine efficacy studies. Here, we evaluated compared the performance seven commercially available enzyme‐linked immunosorbent assay (ELISA)s anti‐severe acute respiratory syndrome corona virus 2 (SARS‐CoV‐2) immunoglobulin G (IgG). ELISAs were with a characterized panel 100 serum samples from qRT‐PCR confirmed...
Dried blood spot (DBS) collection offers various advantages over conventional methods of collection, especially when collecting and transporting samples for a serosurvey. Yet use DBS requires additional processing steps in the laboratory that can add to variability results.
Background India has set a goal to eliminate measles and rubella/Congenital Rubella Syndrome (CRS) by 2023. Towards this goal, conducted nationwide supplementary immunization activity (SIA) with measles-rubella containing vaccine (MRCV) targeting children aged between 9 months <15 years established hospital-based sentinel surveillance for CRS. Reliable data about incidence of CRS is necessary monitor progress towards the elimination goal. Methods We serosurveys in 2019–20 among pregnant...
Monitoring the antibody responses to SARS-CoV-2 infection and its correlation clinical spectrum of disease is critical in understanding progression protection against re-infection. We assessed nucleocapsid (N) receptor-binding-domain spike (SRBD) protein specific IgG neutralizing (NAb) COVID-19 patients up 8 months with diverse spectrum.During first wave pandemic, from 284 patients, 608 samples were collected post infection. The categorized as asymptomatic, symptomatic severe. N SRBD NAb...
India did phased measles-rubella supplementary immunisation activities (MR-SIAs; ie, mass-immunisation campaigns) targeting children aged 9 months to less than 15 years. We estimated seroprevalence before and after the MR-SIAs quantify effect on population immunity identify remaining gaps.Between March 9, 2018 19, 2020 we community-based, cross-sectional serosurveys in four districts MR-SIAs. 30 villages or wards were selected within each district, one census enumeration block from was as...
The pandemic caused by the SARS-CoV-2 has been a threat to humankind due rapid spread of infection and appearance multiple new variants. In present study, we report dynamics persistence immunoglobulin G (IgG) M (IgM) antibodies in asymptomatic symptomatic COVID-19 patients chemiluminescent assay.A total 463 serum samples from 218 PCR-positive were collected over period 124 days post-onset disease (POD). Antibody levels measured chemiluminescence bioanalyzer. Neutralizing antibody titres...
Fingerprick blood spotted onto filter paper offers an alternative to venous for use in population-based surveillance because it is comparatively inexpensive, acceptable, and easy manage the field. Prior studies have shown excellent agreement immunoglobulin G (IgG) antibody detection from dried spots (DBS) samples. However, much of this evidence high-income settings or laboratories where samples were unlikely be exposed extreme temperatures humidity, factors known degrade DBS. We report...
Serological surveillance for vaccine-preventable diseases, such as measles and rubella, can provide direct measures of population immunity across age groups, identify gaps in immunity, document changes over time. Rigorously conducted, representative household serosurveys high-quality estimates with minimal bias. However, they be logistically challenging, expensive, have higher refusal rates than vaccine coverage surveys. This article shares lessons learned through implementing nine rubella...
Abstract Enzyme linked immunosorbent assay (ELISA) plays an important role in laboratory confirmation of congenital rubella syndrome (CRS), postnatal and seroprevalence studies different populations. Variation results are documented for samples tested by commercial kits. The Enzygnost ELISA, widely used the WHO network, is expensive not readily available. In present study, performance Euroimmun ELISA was compared to detection specific IgM IgG antibodies. Two hundred eighty five serum...
Background: India conducted phased measles-rubella supplementary immunization activities (MR-SIAs) targeting children aged 9 months to <15 years from 2017 2019, introducing rubella vaccine into the Universal Immunization Programme. We estimated measles and seroprevalence before after MR-SIAs.Methods: Community-based, cross-sectional serosurveys were in four districts MR-SIAs. Thirty villages or wards selected within each district one census enumeration block of them was as survey cluster....
Abstract Residual blood specimens provide a sample repository that could be analyzed to estimate and track changes in seroprevalence with fewer resources than household-based surveys. We conducted parallel facility community-based cross-sectional serological surveys two districts India, Kanpur Nagar District, Uttar Pradesh, Palghar Maharashtra, before after measles-rubella supplemental immunization activity (MR-SIA) from 2018 2019. Anonymized residual children 9 months younger 15 years of...
Residual blood specimens collected at health facilities may be a source of samples for serosurveys adults, population often neglected in community-based serosurveys. Anonymized residual were from individuals 15 - 49 years age attending two sub-district hospitals Palghar District, Maharashtra, November 2018 to March 2019. Specimens also women enrolled cross-sectional, serosurvey representative the district level that was conducted 2 7 months after specimen collection. tested IgG antibodies...
A growing number of organisations, including medical associations, recommend that research subjects should be given the option being informed about general outcome and results study. We recently completed a study involving nine serosurveys from 2018 to 2020 in five districts India among three age groups (children 9 months < 5 years; 15 years age, women 50 before after measles rubella (MR) vaccination campaigns). In Palghar district Maharashtra all individuals 30 selected clusters were...