A5051868616- Venomous Animal Envenomation and Studies
- Ion channel regulation and function
- Nicotinic Acetylcholine Receptors Study
- Healthcare and Venom Research
- Lipid Membrane Structure and Behavior
- Insect and Pesticide Research
- Toxin Mechanisms and Immunotoxins
- Neurobiology and Insect Physiology Research
- Insects and Parasite Interactions
- Erythrocyte Function and Pathophysiology
- Cell death mechanisms and regulation
- Rabies epidemiology and control
- Animal Ecology and Behavior Studies
- Viral Infections and Vectors
- Bacillus and Francisella bacterial research
Institut Pasteur du Maroc
2002-2022
Institut Pasteur
2013-2014
The present study compared two methods used successfully in a large-scale program for the collection of scorpion venoms, namely milking adult scorpions via manual and electrical stimulation.Our immunobiochemical characterizations clearly demonstrate that regularly applied stimulation obtains venom more easily and, most importantly, greater quantity. Qualitatively, electrically collected showed lack hemolymph contaminants such as hemocyanin. In contrast, obtainment subjects to maximal trauma,...
Scorpion stings and snake bites are major health hazards that lead to suffering of victims high mortality. Thousands injuries associated with such venomous animals occur every year worldwide. In North Africa, more than 100,000 scorpion reported annually. An appropriate determination the 50% lethal doses (LD50) venoms appears be an important step assess (and compare) venom toxic activity. Such LD50 values also commonly used evaluate neutralizing capacity specific anti-venom batches. present...
Hepatocellular carcinoma (HCC) is the most common primary liver cancer in adults, fifth malignancy worldwide and third leading cause of related death. An alternative to surgical treatments drugs, such as sorafenib, commonly used medicine necessary overcome this public health problem. In study, we determine anticancer effect on HCC Moroccan cobra Naja haje venom its fraction obtained by gel filtration chromatography against Huh7.5 cell line. Cells were grown together with WI38 human...
Scorpion envenomation is a serious public health issue. Androctonus mauretanicus ( Am) and Buthus occitanus Bo) are the most dangerous scorpions in Morocco. Despite their medical relevance, no study has yet related kinetics of symptom apparition consequent tissue disorders at same interval post-injection. This work achieved first comparative pathophysiological toxic-symptoms between Am Bo venoms from biochemical, toxicological physiopathological standpoint. The activity subletal dose were...
Androctonus mauretanicus (A. mauretanicus) and Buthus occitanus (B. occitanus) scorpions, which belong to the Buthidae family, are most venomous scorpions in Morocco. For first time, we investigated effects of such scorpion venoms on serum electrolytes subcutaneously injected rabbits. this purpose, 3 groups 6 albinos adult male rabbits (New Zealand) were used experiment. Two given a single subcutaneous injection either crude Am venom (5 μg/kg) or Bo (8 whereas third group (control group)...
The aim of this study is to reveal the biological and toxicological activities Cerastes cerastes Macrovipera mauritanica venoms as well their fractions, immunological activity two monospecific experimental antivenins produced in horse. Our data demonstrated hemorrhagic, necrotic, edematous, myotoxic, coagulant, proteolytic, hemolytic, phospholipasic studied toxic fractions. Microscopically, intradermal muscle sections showed edema, disruption fibers, inflammatory infiltrates. Both antivenoms...
Background: Androctonus mauretanicus (Am ) is one of the most hazardous scorpions in Morocco and has a highly toxic venom responsible for severe cases envenomation. However, few studies have focused on deciphering its proteic composition. Objectives: Herein, we aim to map out complete proteome Am filtrate highlight complexity polymorphism content. This, turn, will lead deeper understanding toxins’ mechanism action help uncover those with therapeutic potential. Methods: Top-down bottom-up...
In Morocco, ophidian envenomations are perpetrated by 7 species of snakes the viper family and a single elapids named Naje haje. Proteomic mapping haje cobra venom shows complex mixture biologically active molecules, which rich in non-enzymatic proteins such as neurotoxins generally - alpha inducing neurotoxic effect attacking central peripheral nervous system causing paralysis. The present work offers study from toxicological physiopathological standpoint. We first determined toxicity Nh...