A5090100430- Complement system in diseases
- Neonatal Respiratory Health Research
- Inflammasome and immune disorders
- Immunotherapy and Immune Responses
- Respiratory Support and Mechanisms
- IL-33, ST2, and ILC Pathways
- Listeria monocytogenes in Food Safety
- Inflammatory mediators and NSAID effects
- Renal Diseases and Glomerulopathies
- Apelin-related biomedical research
- Atherosclerosis and Cardiovascular Diseases
- Chronic Lymphocytic Leukemia Research
- Antibiotic Resistance in Bacteria
- Blood Coagulation and Thrombosis Mechanisms
- Autoimmune Bullous Skin Diseases
- Genital Health and Disease
- Cancer, Stress, Anesthesia, and Immune Response
- Fatty Acid Research and Health
- Infant Nutrition and Health
- melanin and skin pigmentation
- CRISPR and Genetic Engineering
- Innovations in Medical Education
- Bacterial Identification and Susceptibility Testing
- Inflammatory Myopathies and Dermatomyositis
- Biomedical and Engineering Education
Brigham and Women's Hospital
2024-2025
Harvard University
2022-2025
Harvard Affiliated Emergency Medicine Residency
2023
Massachusetts General Hospital
2022
University of Wisconsin–Madison
2015-2020
University of Minnesota
2014
The activity of daptomycin (DAP) against methicillin-resistant Staphylococcus aureus (MRSA) is enhanced in the presence β-lactam antibiotics. This effect more pronounced with antibiotics that exhibit avid binding to penicillin protein 1 (PBP1). Here, we present evidence PBP1 has a significant role responding DAP-induced stress on cell. Expression pbpA transcript, encoding PBP1, was specifically induced by DAP exposure whereas expression pbpB, pbpC, and pbpD, PBP2, PBP3, PBP4, respectively,...
Abstract Granzymes are a family of serine proteases mainly expressed by CD8 + T cells, natural killer and innate-like lymphocytes 1,2 . Although their major role is thought to be the induction cell death in virally infected tumor accumulating evidence suggests some granzymes can regulate inflammation acting on extracellular substrates 2 Recently, we found that majority tissue cells rheumatoid arthritis (RA) synovium, inflammatory bowel disease other inflamed organs express granzyme K (GZMK)...
Adult-onset dermatomyositis (DM) is an autoimmune inflammatory myopathy with distinct cutaneous manifestations and a strong malignancy association. Through comparative analysis lupus erythematosus (CLE), our integrated spatial single-cell transcriptomics revealed unique immune stromal niches associated DM subtypes. Unexpectedly, we found association between cancer-associated skin lesions the presence of dispersed infiltrates enriched macrophages, CD8+ T cells, plasma B cells preserved...
The influence of cell death on adaptive immunity has been studied for decades. Despite these efforts, the intricacies how various pathways shape immune responses in context infection remain unclear, particularly with regard to more recently discovered such as pyroptosis. emergence Listeria monocytogenes a promising immunotherapeutic platform demands thorough understanding induced influences generation CD8+ T-cell-mediated responses. To begin address this question, we designed strains L. that...
Dual degree program MD/PhD candidates typically train extensively in basic science research and clinical medicine, but often receive little formal experience or mentorship translational research. To address this educational curricular gap, the University of Wisconsin Medical Scientist Training Program partnered with Institute for Clinical Translational Research to create a new physician-scientist preceptorship This six-week apprentice-style learning experience—guided by faculty...
Background. Sipuleucel T, an autologous cell-based vaccine targeting prostatic acid phosphatase (PAP), has demonstrated efficacy for the treatment of advanced prostate cancer. DNA vaccines encoding PAP and live attenuated Listeria have entered clinical trials patients with cancer, advantages in terms eliciting predominantly Th1-biased immunity. In this study, we investigated whether immunogenicity anti-tumor a vaccine, each PAP, could be enhanced by using them heterologous prime/boost...
Abstract The cytokine IL-1β plays a central role in inflammatory responses that are initiated by microbial challenges, as well those due to endogenous processes (often called sterile inflammation). secretion occurs independently of stimulation is typically associated with the presence alarmins, such extracellular ATP (an indicator cytopathic damage). In this study, we show IL-2–activated human invariant NKT (iNKT) cells stimulate protein peripheral blood monocytes manner requires neither...
Abstract Nonsteroidal anti-inflammatory drugs (NSAIDs) inhibit cyclooxygenase (COX) activity and are commonly used for pain relief fever reduction. NSAIDs following childhood vaccinations cancer immunotherapies; however, how influence the development of immunity these therapies is unknown. We hypothesized that would modulate an immune response to Listeria monocytogenes–based immunotherapy. Treatment mice with nonspecific COX inhibitor indomethacin impaired generation cell-mediated immunity....
Discoid lupus erythematosus (DLE) is a form of chronic cutaneous that typically presents on the head and neck results in scarring.1 DLE may rarely present as eyelid edema erythema (periorbital DLE), variant reported literature.2
Abstract Background Hypergammaglobulinemic purpura of Waldenström (HGPW), a rare cutaneous eruption characterized by the triad recurrent episodes lower extremity petechiae, symptoms stinging and burning, edema, is poorly described in children. Some children have been reported to follow benign course, while others are eventually diagnosed with fulminant rheumatologic disease. Objectives To determine distinguishing features HGPW including spectrum disease manifestations clinical outcomes....
Ovarian cancer rarely metastasizes to the skin, but when cutaneous metastases occur, typical location is within umbilicus, commonly known as a Sister Mary Joseph nodule. Extraabdominal from ovarian are extremely rare. Here, we report series of 3 patient cases at single institution who presented with extraabdominal metastases. We discuss potential mechanisms for involvement well histopathology, treatments, and outcomes in patients
Abstract Listeria monocytogenes is a genetically tractable Gram-positive intracellular bacterium that activates robust cell-mediated immune response capable of breaking self-tolerance. These properties contribute to its success as cancer immunotherapeutic platform; however, despite these attributes, the specific mechanisms by which L. stimulates adaptive remain largely unknown. We hypothesized cytosolic innate activation, specifically inflammasome would be critical for triggering CD8+ T-cell...
Abstract The bacterial pathogen Listeria monocytogenes (Lm) is being developed as a cancer immunotherapeutic platform due to its ability elicit robust CD8+ T cell responses. Though the role of cytokines in Lm-stimulated immunity well studied, eicosanoids, lipid modulators inflammation, unclear. We previously demonstrated that cyclooxygenase-2 (COX-2) dependent prostaglandin E2 (PGE2) essential for optimal T-cell priming. Antigen specific cells and protective were decreased following either...