A5009693043- Lipid Membrane Structure and Behavior
- Sphingolipid Metabolism and Signaling
- Surfactants and Colloidal Systems
- Erythrocyte Function and Pathophysiology
- RNA Interference and Gene Delivery
- Nanopore and Nanochannel Transport Studies
- Nanoparticle-Based Drug Delivery
- Lysosomal Storage Disorders Research
- Cellular transport and secretion
- Force Microscopy Techniques and Applications
- Hydrogels: synthesis, properties, applications
- Bacteriophages and microbial interactions
- Porphyrin and Phthalocyanine Chemistry
- Nanocluster Synthesis and Applications
- Bacterial Infections and Vaccines
- Advanced NMR Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- SARS-CoV-2 and COVID-19 Research
- Neonatal Health and Biochemistry
- Pancreatitis Pathology and Treatment
- Cancer, Lipids, and Metabolism
- Proteins in Food Systems
- Blood properties and coagulation
- DNA and Nucleic Acid Chemistry
Instituto Biofisika
2016-2024
University of the Basque Country
2013-2024
Universidad Autónoma de Madrid
2024
Fundación Biofísica Bizkaia
2015-2020
Supported planar bilayers (SPBs) on mica substrates have been studied at 23 °C under atomic force microscopy (AFM)-based surface topography and spectroscopy with two main objectives: (i) to characterize palmitoylceramide (pCer)-induced gel (Lβ) domains in binary mixtures either its sphingolipid relative palmitoylsphingomyelin (pSM) or the glycerophospholipid dipalmitoylphosphorylcholine (DPPC) (ii) evaluate effects of incorporating cholesterol (Chol) into previous terms Cer Chol cooperation...
We studied the properties of bilayers formed by ether-and ester-containing phospholipids, whose hydrocarbon chains can be either linear or branched, using sn-1,2 dipalmitoyl, dihexadecyl, diphytanoyl, and diphytanyl phosphatidylcholines (DPPC, DHPC, DPhoPC, DPhPC, respectively) pure in binary mixtures. Differential scanning calorimetry confocal fluorescence microscopy giant unilamellar vesicles concurred showing that equimolar mixtures branched lipids gave rise to gel/fluid phase coexistence...
The mechanisms of Pb(II) toxicity have been studied in human red blood cells using confocal microscopy, immunolabeling, fluorescence-activated cell sorting and atomic force microscopy. process follows a sequence events, starting with calcium entry, followed by potassium release, morphological change, generation ceramide, lipid flip-flop finally lysis. Clotrimazole blocks channels the whole is inhibited. Immunolabeling reveals ceramide-enriched domains linked to while use neutral...
Free volume voids in lipid bilayers can be measured by positron annihilation lifetime spectroscopy (PALS). This technique has been applied, together with differential scanning calorimetry and molecular dynamics (MD) simulations, to study the effects of cholesterol (Chol) ceramide (Cer) on free sphingomyelin (SM) or dipalmitoylphosphatidylcholine (DPPC) bilayers. Binary samples Chol were studied (DPPC:Chol 60:40, SM:Chol 60:40 mol ratio), no phase transition was detected 20-60 °C range,...
Toxicity evaluation for the proper use of graphene oxide (GO) in biomedical applications involving intravenous injections is crucial, but GO circulation time and blood interactions are largely unknown. It thought that may cause physical disruption (hemolysis) red cells. The aim this work to characterize interaction with model cell membranes knowledge improve hemocompatibility. We have found interacts both neutral negatively charged lipid membranes; binding decreased beyond a certain...
The effects of increasing amounts palmitoylceramide (pCer) on human red blood cell lipid membranes have been studied using atomic force microscopy supported bilayers, in both imaging (bilayer thickness) and force-spectroscopy (nanomechanical resistance) modes. Membranes appeared homogeneous with pCer concentrations up to 10 mol % because the high concentration cholesterol (Chol) present membrane (∼45 %). However, presence at 30 gave rise a clearly distinguishable segregated phase...
The effects of C24:1 sphingolipids have been tested in phospholipid bilayers containing cholesterol. Confocal microscopy, differential scanning calorimetry, and atomic force microscopy imaging curves used. More precisely, the ceramide (nervonoyl ceramide, nCer) were evaluated compared to those C16:0 (palmitoyl pCer) composed basically dioleoylphosphatidylcholine, sphingomyelin (either C24:1, nSM or C16:0, pSM) Combination equimolecular amounts also studied under same conditions. Results show...
Cell membranes have been proposed to be laterally inhomogeneous, particularly in the case of mammalian cells, due presence "domains" enriched sphingolipids and cholesterol (Chol). Among membrane sphingolipids, sphingomyelin (SM) cell plasma is known degraded ceramide (Cer) by acid sphingomyelinases under stress conditions. Since (Chol) abundant membrane, study ternary mixtures SM:Chol:Cer interesting from point view biophysics, it might physiologically relevant. In previous studies, we...
This work intends to describe the physical properties of red blood cell (RBC) membranes in obese adults. The hypothesis driving this research is that obesity, addition increasing amount body fat, will also modify lipid composition cells other than adipocytes. Forty-nine control volunteers (16 male, 33 female, BMI 21.8 ± 5.6 and 21.5 4.2 kg/m2, respectively) 52 subjects male 36 38.2± 11.0 40.7 8.7 were examined. two techniques applied atomic force microscopy (AFM) spectroscopy mode, which...
We have measured by positron annihilation lifetime spectroscopy (PALS) that ceramide increases the size of free volume holes in DPPC lipid membranes.
RTX (Repeats in ToXin) pore-forming toxins constitute an expanding family of exoproteins secreted by many Gram-negative bacteria and involved infectious diseases caused said pathogens. Despite the relevance host/pathogen interactions, structure characteristics lesions formed these remain enigmatic. Here, we capture first direct nanoscale pictures lytic pores toxin, Adenylate cyclase (ACT), whooping cough bacterium Bordetella pertussis. We reveal that ACT associates into growing-size...
The discovery of a novel sphingolipid subclass, the (1-deoxy)sphingolipids, which lack 1-hydroxy group, attracted considerable attention in last decade, mainly due to their involvement disease. They differed physico-chemical properties from canonical (or 1-hydroxy) sphingolipids and they were more toxic when accumulated cells, inducing neurodegeneration other dysfunctions. (1-Deoxy)ceramides, (1-deoxy)dihydroceramides, (1- deoxymethyl)dihydroceramides, latter two containing saturated...
Abstract Coronavirus stands for a large family of viruses characterized by protruding spikes surrounding lipidic membrane adorned with proteins. The present study explores the adhesion transmissible gastroenteritis coronavirus (TGEV) particles on variety reference solid surfaces that emulate typical virus‐surface interactions. Atomic force microscopy informs about trapping effectivity and shape virus envelope each surface, revealing deformation TGEV spans from 20% to 50% in diameter. Given...
This study was aimed at preparing and characterizing plasma membranes (PM) from Chinese Hamster Ovary (CHO) cells. Two methods of PM preparation were applied, one based on adhering cells to a poly-lysine-coated surface, followed by hypotonic lysis removal intracellular components, so that patches remain adhered each other, second consisting bleb induction in cells, separation giant membrane vesicles (GPMV). Both gave rise sufficient amounts allow biophysical biochemical characterization....
Sphingolipids (SL) are ubiquitous in mammalian cell membranes, yet there is little data on the behavior of cells under SL-restriction conditions. LY-B derive from a CHO linein whichserine palmitoyl transferase (SPT), thus de novo SL synthesis, suppressed, while maintaining capacity taking up and metabolizing exogenous sphingoid bases culture medium. In this study, were adapted to grow fetal bovine serum (FBS)-deficient medium avoid external uptake lipids. The lowest FBS concentration that...
Abstract Suppression of a specific gene effect can be achieved by genetic as well chemical methods. Each approach may hide unexpected drawbacks, usually in the form side effects. In present study, inhibitor myriocin was used to block serine palmitoyltransferase (SPT), first enzyme sphingolipid synthetic pathway, CHO cells. The subsequent biophysical changes plasma membranes were measured and compared with results obtained genetically modified cell line containing defective SPT (the LY-B...