A5023030037- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- MicroRNA in disease regulation
- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
- Neuroscience and Neural Engineering
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Planarian Biology and Electrostimulation
- 3D Printing in Biomedical Research
- Nerve injury and regeneration
Dartmouth College
2024-2025
Wellcome/MRC Cambridge Stem Cell Institute
2019-2024
University of Cambridge
2019-2024
Dartmouth Hospital
2024
Medical Research Council
2019-2021
Montreal Neurological Institute and Hospital
2019
McGill University
2019
Oligodendrocyte progenitor cells (OPCs), which differentiate into myelinating oligodendrocytes during CNS development, are the main proliferative in adult brain. OPCs conventionally considered a homogeneous population, particularly with respect to their electrophysiological properties, but this has been debated. We show, by using single-cell recordings, that start out as population become functionally heterogeneous, varying both within and between brain regions age. These changes correlate...
Myelinating oligodendrocytes die in human disease and early aging. Despite this, the mechanisms that underly oligodendrocyte death are not resolved it is also clear whether these change as lineage cells undergoing differentiation maturation. Here, we used a combination of intravital imaging, single-cell ablation, cuprizone-mediated demyelination, both female male mice, to discover maturation dictates dynamics cell death. After phototoxic damage, precursor underwent programmed within hours,...
Oligodendrocyte precursor cells (OPCs) differentiate during postnatal development into myelin-forming oligodendrocytes, in a process distinguished by substantial changes morphology and the onset of myelin gene expression. A mammalian-specific CNS gene, tmem10, also called Opalin, encodes type 1 transmembrane protein that is highly upregulated early stages OPC differentiation; however, function for TMEM10 has not yet been identified. Here, consistent with previous studies, we detect mouse...
Oligodendrocytes are generated throughout life and in neurodegenerative conditions from brain resident oligodendrocyte precursor cells (OPCs). The transition OPC to involves a complex cascade of molecular morphological states that position the cell make fate decision integrate as myelinating or die through apoptosis. Oligodendrocyte maturation impacts death mechanisms occur degenerative conditions, but it is unclear if how machinery changes OPCs into oligodendrocytes. Here, we discovered...
Abstract In the central nervous system, oligodendrocyte precursor cells (OPCs) proliferate and differentiate into myelinating oligodendrocytes throughout life, allowing for ongoing myelination myelin repair. With age, differentiation efficacy decreases repair fails; therefore, recent therapeutic efforts have focused on enhancing differentiation. Many cues are thought to regulate OPC differentiation, including neuronal activity, which OPCs can sense respond via their voltage-gated ion...
Single-cell electrophysiological recordings combined with dye loading and immunohistochemistry provide unparalleled single-cell resolution of cell physiology, morphology, location, protein expression. When correlated bulk RNA sequencing, these data can define identity function. Here, we describe a protocol to prepare acute brain slices from embryonic postnatal mice for whole-cell patch clamp, post-hoc immunohistochemistry, isolation sequencing. While focus on oligodendrocyte precursor cells,...
ABSTRACT Oligodendrocytes are generated throughout life and in neurodegenerative conditions from brain resident oligodendrocyte precursor cells (OPCs). The transition OPC to involves a complex cascade of molecular morphological states that position the cell make fate decision integrate as myelinating or die through apoptosis. Oligodendrocyte maturation impacts death mechanisms occur degenerative conditions, but it is unclear if how machinery changes OPCs into oligodendrocytes. Here, we...