A5063129310- Circadian rhythm and melatonin
- Light effects on plants
- Plant Molecular Biology Research
- Algal biology and biofuel production
- Sleep and Wakefulness Research
- Phytase and its Applications
- RNA Interference and Gene Delivery
- Computational Drug Discovery Methods
- ATP Synthase and ATPases Research
- Muscle metabolism and nutrition
- Cell Adhesion Molecules Research
- Mosquito-borne diseases and control
- interferon and immune responses
- Angiogenesis and VEGF in Cancer
EpiCypher (United States)
2025
University of California, Santa Cruz
2018-2023
North Carolina State University
2010
Significance Our innate circadian clocks control myriad aspects of behavior and physiology. Disruption our by shift work, jet lag, or inherited mutation leads to metabolic dysregulation contributes diseases, including diabetes cancer. A central step in clock is phosphorylation the PERIOD 2 (PER2) protein. Here we conclusively identify elusive PER2 priming kinase find it be well-known kinase, casein 1 (CK1). Surprisingly, different forms CK1 have differing abilities phosphorylate site, adding...
PERIOD (PER) and Casein Kinase 1δ regulate circadian rhythms through a phosphoswitch that controls PER stability repressive activity in the molecular clock. CK1δ phosphorylation of familial advanced sleep phase (FASP) serine cluster embedded within 1 binding domain (CK1BD) mammalian PER1/2 inhibits its on phosphodegrons to stabilize extend period. Here, we show phosphorylated FASP region (pFASP) PER2 directly interacts with CK1δ. Co-crystal structures conjunction dynamics simulations reveal...
Post-translational control of PERIOD stability by Casein Kinase 1δ and ε (CK1) plays a key regulatory role in metazoan circadian rhythms. Despite the deep evolutionary conservation CK1 eukaryotes, little is known about its regulation factors that influence substrate selectivity on functionally antagonistic sites directly period. Here we describe molecular switch involving highly conserved anion binding site CK1. This controls conformation kinase activation loop determines which mammalian...
ADP-ribosylation, characterized by the addition of adenosine diphosphate ribose, can occur in both monomeric (MARylation) and polymeric (PARylation) forms. Little is known about specific contributions MARylation PARylation to cellular processes due a lack tools for jointly investigating these individual We present novel mass spectrometry (MS)-based proteomics approach that preserves information native ADP-ribosylation form associated with modification site within single experiment. Our...
Bafilomycin A(1) is a specific inhibitor of the vacuolar-ATPase (V-ATPase), which responsible for pH homeostasis cell and acidification endosomes. has been commonly used as method inhibition infection by viruses known or suspected to follow path receptor-mediated endocytosis low-pH-mediated membrane fusion. The exact entry Sindbis virus, prototype alphavirus, remains undetermined. To further investigate role V-ATPase in virus infection, effects bafilomycin on BHK insect cells were studied....
Summary Post-translational control of PERIOD stability by Casein Kinase 1δ and ε (CK1) plays a key regulatory role in metazoan circadian rhythms. Despite the deep evolutionary conservation CK1 eukaryotes, little is known about its regulation factors that influence substrate selectivity on functionally antagonistic sites directly period. Here we describe molecular switch involving highly conserved anion binding site CK1. This controls conformation activation loop to define mammalian PER2,...
Summary PERIOD (PER) and Casein Kinase 1δ regulate circadian rhythms through a phosphoswitch that controls PER stability repressive activity in the molecular clock. CK1δ phosphorylation of Familial Advanced Sleep Phase (FASP) serine cluster embedded within 1 binding domain (CK1BD) mammalian PER1/2 inhibits its on phosphodegrons to stabilize extend period. Here, we show phosphorylated FASP region (pFASP) PER2 directly interacts with CK1δ. Co-crystal structures conjunction accelerated dynamics...