Breast cancer stem cells (BCSCs) can be enriched by culturing of in non-adherent non-differentiating conditions. However, mammospheres from primary breast tumors are costly and difficult to control. In order overcome problems associated with using human tissues, continuous cell lines have been developed various sources.In this study, a luminal subtype line MCF-7 basal MDA-MB-231 were chosen. We explored the optimal system for BCSCs two tumors. Then, mammosphere formation efficiency (MFE),...

Abstract Decellularized extracellular matrix (ECM) scaffolds from human adipose tissue, characterized by impressive adipogenic induction ability, are promising for soft tissue augmentation. However, autologous limited the availability of resources and time necessary scaffold fabrication. The objective current study was to investigate properties hydrogels decellularized porcine (HDPA). HDPA induced differentiation adipose‐derived stem cells (ADSCs) in vitro , with significantly increased...

// Shen-li Tang 1, 2 , Ji-qiao Yang Zheng-gui Du Qiu-wen Tan Yu-ting Zhou Di Zhang and Qing Lv 1 Department of Breast Surgery, West China Hospital/West School Medicine, Sichuan University, 610041, P.R. Laboratory Disease, Correspondence to: Lv, email: lvqingwestchina@163.com Keywords: breast cancer, invasive micropapillary carcinoma, locoregional recurrence, prognostic factors, survival Received: September 20, 2016     Accepted: February 21, 2017    ...

Mimicking the native microenvironment is vital for tumor engineering. Breast cancer a highly heterogeneous disease with various molecular subtypes exhibiting distinct biological behaviors and treatment responsiveness. The heterogeneity of extracellular matrix (ECM) breast has remained largely unexplored underestimated. present study addressed this issue by comparing composition, architecture, functional roles ECMs derived from cancers two subtypes, which are luminal-A (less aggressive,...

Corticosteroids are recommended as a first-line treatment for idiopathic granulomatous mastitis (IGM), disease that usually occurs in young women. Corticosteroid phobia is fear of corticosteroids and one the main reasons poor compliance. Despite increasing recognition corticosteroid phobia, there has been lack studies on this issue IGM. This study was designed to investigate prevalence degree IGM patients. A cross-sectional survey conducted among patients who were receiving at West China...

Adjuvant chemotherapy may cause alterations in serum lipids postoperative breast cancer (BC) patients, but the specific caused by different regimens remain unclear. The aim of this study was to investigate status pre- and post-chemotherapy compare side effects on lipid.We retrospectively analysed lipid profiles 1934 consecutive BC patients who received one following regimens:The levels triglycerides (TG), total cholesterols (TC), low-density lipoprotein (LDL-C) were significantly elevated...

Positive lymph node ratio (LNR), defined as of positive nodes to all removed, is a powerful prognostic factor in invasive breast cancer. Here we focused on the impact negative (NLN) count prediction value LNR cancer survival.Of 929 patients were enrolled our retrospective study. We use Kaplan-Meier calculate 5-year overall survival (OS) according different clinicopathologic parameters. The NLN and OS was examined.The optimal cutoff designated 9. Five-year 77.0% 95.0% with 0-9 ≥10,...

The aim of this study is to report our experience with ductoscopic evaluation for screening patients nipple discharge and evaluate any potential indications benefits ductoscopy. From January 2010 December 2012, 419 female were enrolled in study. All involved showed no mass ultrasound mammography. Data concerning age, clinical characteristics discharge, cytology, postsurgical diagnosis, complications statistically analyzed. Ductoscopy examinations completed 405 (96.66%). For these patients,...

Abstract Background Extensive studies have confirmed the efficacy of taxanes in combination with anthracycline-based chemotherapy on breast cancer. However, few assessed weekly taxane–anthracycline regimens locally advanced This study was to compare and safety a regimen those tri-weekly patients Methods Patients cancer were randomized receive 4–6 cycles neoadjuvant 5-fluorouracil–epirubicin–cyclophosphamide (FEC) or paclitaxel–epirubicin (PE) regimen. The primary endpoint pathologic complete...

Objective: The application of sentinel lymph node biopsy (SLNB) in elderly patients with early breast cancer remains somewhat controversial. This study aimed to establish individualized nomograms predict survival outcomes and without SLNB find out which could avoid SLNB. Methods: A total 39,962 ≥70-year-old diagnosed T1-T2 2010-2015 were included from the Surveillance, Epidemiology, End Results (SEER) program divided into training set (n = 29,971) validation 9,991). Axillary surgery was not...

Idiopathic granulomatous mastitis (IGM) is a rare, benign, but locally aggressive breast disease. Steroids are widely used as breast-conserving option, however, the response rate of steroids varies in reported studies, well its different usage. This prospective observational cohort study aimed to report outcomes methylprednisolone for IGM treatment.From Aug 2019 Dec 2021, clinicopathological information 156 patients who sought treatment at West China Hospital was prospectively collected. A...

Our previous study fabricated decellularized porcine muscle tissues (DPMTs) and demonstrated that DPMTs with few cell residues possess highly preserved protein components good biocompatibility. In the physical state, skeletal equips an abundant vascular network due to vast demand of energy from aerobic metabolism. Vascular bioactive factors which are rich in may contribute angiogenic effect DPMTs. However, implanting vivo a less invasive way is unfeasible. Hence, purpose this was fabricate...

Abstract Lipofilling is a popular technique for soft tissue augmentation, limited by unpredictable graft survival. This study aimed at exploring the effect of hydrogel from acellular porcine adipose (HAPA) on angiogenesis and survival used lipofilling. The HAPA adipose-derived stem cells (ADSCs) proliferation, adipogenic differentiation, vascular endothelial growth factor (VEGF) secretion were evaluated in hypoxia normoxia vitro . For vivo study, with phosphate buffered saline, ADSCs, (with...

To explore an optimized protocol of decellularization to fabricate ideal scaffold derived from porcine skeletal muscle acellular matrix.Serial-step homogenating-milling-detergent method was used decellularized tissue (DPMT) native adult pig waist. Histological assess the effects and degreasing. Sirius red staining analyze collagen components. Scanning electron microscopy, BCA assay, PicoGreen assay were evaluate ultrastructure, total protein content, DNA content in DPMT. The adipose stem...

To overview the theoretical basis and research status of prepectoral implant-based breast reconstruction.The domestic foreign researches on application reconstruction in were retrospectively analyzed. The basis, clinical advantages, limitations this technique summarized future development trend field was discussed.The recent advances cancer oncology, materials concept oncology have provided a for reconstruction. selection patients experience surgeons are crucial postoperative outcomes. Ideal...

Oncoplastic breast-conserving surgery (OPBS) is the evolution of conventional (CBCS); however, data from studies comparing patients who received two surgical procedures are limited. A comparison differences in terms patient characteristics, tumor-nipple distance, volume resected breast tissue, tumor and postoperative appearance between undergoing OPBS CBCS was carried out this study, enhancing evidence base for by widening indications improving satisfaction.From January 2020 to April 2022,...