A5010483754- Microfluidic and Bio-sensing Technologies
- Additive Manufacturing and 3D Printing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Electrowetting and Microfluidic Technologies
- Protein purification and stability
- Cellular Mechanics and Interactions
- Advanced Materials and Mechanics
- Nanopore and Nanochannel Transport Studies
- Computer Graphics and Visualization Techniques
- Nanomaterials for catalytic reactions
- Anatomy and Medical Technology
- Fluid Dynamics and Turbulent Flows
- Laser-Ablation Synthesis of Nanoparticles
- Rheology and Fluid Dynamics Studies
- Polymer composites and self-healing
- Extracellular vesicles in disease
- SARS-CoV-2 and COVID-19 Research
- Pickering emulsions and particle stabilization
- thermodynamics and calorimetric analyses
- Electrohydrodynamics and Fluid Dynamics
- vaccines and immunoinformatics approaches
- Modular Robots and Swarm Intelligence
- Single-cell and spatial transcriptomics
Lawrence Livermore National Laboratory
2014-2024
University of California, Berkeley
2017
Boston University
2014-2015
Lawrence Livermore National Security
2015
Volumetric additive manufacturing (VAM) forms complete 3D objects in a single photocuring operation without layering defects, enabling printed polymer parts with mechanical properties similar to their bulk material counterparts. This study presents the first report of VAM-printed thiol-ene resins. With well-ordered molecular networks, chemistry accesses materials wide range properties, moving VAM beyond limitations commonly used acrylate formulations. Since free-radical polymerization is not...
The COVID-19 pandemic underscored the promise of monoclonal antibody-based prophylactic and therapeutic drugs
Shape memory polymers (SMPs) capable of recovering from a deformed state through heating were 3D printed using volumetric additive manufacturing, which enabled the layerless, single-step fabrication self-standing tripod and actuating gripper structures.
We present a new approach to acoustofluidic device design with secondary channel separated from the main by thin wall. This allows off-center placement of acoustic nodes, which enables high-efficiency and high-throughput separation cell-scale objects.
We conduct numerical simulations of acoustic focusing in dense suspensions to map the design space acoustically patterned materials and understand relationships between input parameters, structural features, functional properties. develop closed-form expressions for forces on particles, enabling rapid simulation thousands find excellent agreement with experimentally focused patterns over a range conditions. geometrical microstructural features particle their dependence processing parameters....
Abstract Additive manufacturing of freeform structures containing multiple materials with deterministic spatial arrangement and interactions remains a challenge for most 3D printing processes, due to complex fabrication tool requirements limitations in printability some material classes. Here, versatile method is reported produce architected composites using the concept cellular fluidics, which lattices unit cells are used as templating scaffolds guide flowable infill programmed pattern,...
A major advantage of microfluidic devices is the ability to manipulate small sample volumes, thus reducing reagent waste and preserving precious sample. However, achieve robust manipulation it necessary address device integration with macroscale environment. To realize repeatable, sensitive particle separation devices, this protocol presents a complete automated integrated platform that enables precise processing 0.15-1.5 ml samples using devices. Important aspects system include modular...
We report a two-channel fluidic device that allows geometry-independent tuning of acoustic nodes for high-throughput particle sorting.
Previously, we discovered [Mariella et al., J. Appl. Phys. 114, 014904 (2013)] that modest-fluence/modest-intensity 351-nm laser pulses, with insufficient fluence/intensity to ablate rock, mineral, or concrete samples via surface vaporization, still removed the material from water-submerged target confinement of material, and then dispersed at least some into water as a long-lived suspension nanoparticles. We called this new process, which appears include generation larger colorless...
Volumetric additive manufacturing is a novel 3D printing method to form parts in single exposure, contrast traditional stereolithography which builds layer-by-layer. Photopolymerizable resin exposed with grayscale images from different angles over 360 degrees deliver distribution of light energy cures the desired part. The exposure are calculated using computed tomography (CT), same principles used convert X-ray obtained during computerized axial (CAT) scan reconstruct volume patient. A...
A major advantage of microfluidic devices is the ability to manipulate small sample volumes, thus reducing reagent waste and preserving precious sample. However, achieve robust manipulation it necessary address device integration with macroscale environment. To realize repeatable, sensitive particle separation devices, this protocol presents a complete automated integrated platform that enables precise processing 0.15–1.5 ml samples using devices. Important aspects system include modular...
Rapid, high throughput measurements of biomolecular interactions are essential across medicine and bioscience. Traditional methods for affinity-screening proteins require a long costly process involving cell-based expression, purification, titration multiple concentrations to arrive at binding curve. In contrast, we have developed fast simple approach that yields wealth information about the expression protein its characteristics, all in "one-pot reaction" done under several hours without...
Volumetric additive manufacturing (VAM) is an emerging approach for photopolymer-based 3D printing that produces complex structures in a single step, rather than from layer-by-layer assembly. In article number 2003376, Maxim Shusteff and co-workers adapt thiol-ene resins VAM by incorporating threshold response using polymerization inhibitor. Due do the uniform network formation, broad range of mechanical properties can be accessed.
In this study, we demonstrate a new high temperature flow synthesis system for magnetite nanoparticle clusters. We find that successful of clusters is dependent on residence time in the reaction chamber and linear speed. The long times elevated temperatures required, combined with resulting slurry formed nanocluster synthesis, made challenging to adapt system. However, proper design continuous platform parameter control allows adoption even difficult solvothermal processes. discuss...
The utility and accuracy of computational modeling often requires direct validation against experimental measurements. work presented here is motivated by taking a combined approach to determine the ability large-scale fluid dynamics (CFD) simulations understand predict circulating tumor cells in clinically relevant environments. We use stroboscopic light sheet fluorescence imaging track paths measure velocities fluorescent microspheres throughout human aorta model. Performed over complex...
<div>In this study, we demonstrate a new high temperature flow synthesis system for magnetite nanoparticle clusters. We find that successful of clusters is dependent on residence time in the reaction chamber and linear speed. The long times elevated temperatures required, combined with resulting slurry formed nanocluster synthesis, made challenging to adapt system. However, proper design continuous platform parameter control allows adoption even difficult solvothermal processes....