A5047844641- Muscle Physiology and Disorders
- Genetics, Aging, and Longevity in Model Organisms
- Additive Manufacturing and 3D Printing Technologies
- Tissue Engineering and Regenerative Medicine
- Laser Material Processing Techniques
- Geophysical Methods and Applications
- Virus-based gene therapy research
- Ion channel regulation and function
- Surface Roughness and Optical Measurements
- Thin-Film Transistor Technologies
- Electrokinetic Soil Remediation Techniques
- Bacterial Infections and Vaccines
- Laser-induced spectroscopy and plasma
- Diamond and Carbon-based Materials Research
- Reproductive Biology and Fertility
- High-Temperature Coating Behaviors
- Circadian rhythm and melatonin
- Pneumonia and Respiratory Infections
- Manufacturing Process and Optimization
- Heat shock proteins research
- Heat and Mass Transfer in Porous Media
- Advancements in Photolithography Techniques
- Belt Conveyor Systems Engineering
- Nuclear and radioactivity studies
- Real-Time Systems Scheduling
Washington University in St. Louis
2023
Nationwide Children's Hospital
2010-2017
The Ohio State University
2008-2012
nLIGHT (United States)
2009
Georgia Institute of Technology
2003-2008
Abbott (United States)
2005
Auburn University
2002
Idaho National Laboratory
1988-1990
United States Nuclear Regulatory Commission
1988
Knexus Research (United States)
1988
Limb-girdle muscular dystrophies are a genetically diverse group of diseases characterized by chronic muscle wasting and weakness. Recessive mutations in ANO5 (TMEM16E) have been directly linked to several clinical phenotypes including limb-girdle dystrophy type 2L Miyoshi myopathy 3, although the pathogenic mechanism has remained elusive. is member Anoctamin/TMEM16 superfamily that encodes both ion channels regulators membrane phospholipid scrambling. The phenotypic overlap myopathies with...
Objective Dysferlinopathies are a family of untreatable muscle disorders caused by mutations in the dysferlin gene. Lack protein results progressive dystrophy with chronic fiber loss, inflammation, fat replacement, and fibrosis; leading to deteriorating weakness. The objective this work is demonstrate efficient safe restoration expression following gene therapy treatment. Methods Traditional restricted packaging capacity limit adeno-associated virus (AAV), however, use dual vector strategy...
Dysferlinopathies comprise a family of disorders caused by mutations in the dysferlin (DYSF) gene, leading to progressive dystrophy characterized chronic muscle fiber loss, fat replacement, and fibrosis. To correct underlying histopathology function, expression full-length DYSF is required. Dual adeno-associated virus vectors have been developed, defined region homology, serve as substrate for reconstitution full 6.5 kb cDNA. Previous work studied efficacy this treatment through...
Nontypeable Haemophilus influenzae (NTHi) causes pulmonary infections in patients with chronic obstructive disease and other mucociliary clearance defects. Like many bacteria inhabiting mucosal surfaces, NTHi produces lipooligosaccharide (LOS) endotoxins that lack the O side chain. Persistent populations express a discrete subset of LOS glycoforms, including those containing phosphorylcholine (PCho). In this study, we compared two strains isogenic mutants lacking PCho for from mice following...
The pore-forming S. aureus α-toxin (Hla) contributes to virulence and disease pathogenesis. While high concentrations of toxin induce cell death, neutrophils exhibit relative resistance lysis, suggesting that the action Hla may not be solely conferred by lytic susceptibility. Using intravital microscopy, we observed disrupts neutrophil localization clustering early in infection. forms a narrow, ion-selective pore, dysregulate calcium or other ions impair function. We found sub-lytic did...
The development of a reworkable underfill system for flip chip assembly is described. materials selection process, information on the process rework and pertinent reliability data are
Heterochronic genes function to ensure the timing of stage-specific developmental events in C. elegans. Mutations these cause certain programs be executed a precocious or retarded manner. Canonical (loss-of-function) and (gain-of-function) mutations lin-14 gene lead elimination reiteration larval cellular events. Here, we describe hypomorphic, missense allele lin-14, sa485. lin-14(sa485) hermaphrodites pass through normal stages, but exhibit asynchrony between vulval gonadal maturation L4...
Laser diode reliability depends on both power and spectral stability over time. This report examines cases in which corrosion ionic deposition resulted wavelength shifts from less than 1 nm to greater 7 60 - 100W bars microchannel coolers. Both seemed be exacerbated by frequent and/or lengthy periods of stagnation the DI water system. Analytical results including SEM images FIB cross-sections illustrate deposits up several microns thickness dielectric (oxide) material, as well voiding caused...
Laser chemical vapor deposition (LCVD) as a manufacturing process holds the potential to build compositionally and geometrically unique objects. Georgia Tech's LCVD system has been used in past create three‐dimensional laminate structures out of carbon. Recently molybdenum boron nitride were successfully deposited upgrades have allowed for higher spatial resolutions more varied geometric capabilities. Upgrades include addition fourth linear stage implementation an argon ion laser. Detailed...
Others have used Laser Chemical Vapor Deposition (LCVD) to create 3-D fibrous structures and helical springs. Current research efforts focus on the creation of more advanced three-dimensional carbon objects through use multi-layered deposition. Multi-layered require an understanding interlayer adhesion propagation geometric anomalies multiple layers. An important aspect in minimizing these shape is implementation closed loop temperature control. Several laminated are presented with...