A5013144251- Environmental DNA in Biodiversity Studies
- Identification and Quantification in Food
- Species Distribution and Climate Change
- Bird parasitology and diseases
- Genomics and Phylogenetic Studies
- Vector-borne infectious diseases
- Forest Ecology and Biodiversity Studies
- Study of Mite Species
- Diptera species taxonomy and behavior
- Parasite Biology and Host Interactions
- Turtle Biology and Conservation
- Forensic Entomology and Diptera Studies
- Forest Insect Ecology and Management
- Viral Infections and Vectors
- Plant and animal studies
- Isotope Analysis in Ecology
National University of Singapore
2019-2024
DNA barcodes are a useful tool for discovering, understanding, and monitoring biodiversity which critical tasks at time of rapid loss. However, widespread adoption requires cost-effective simple barcoding methods. We here present workflow that satisfies these conditions. It was developed via "innovation through subtraction" thus minimal lab equipment, can be learned within days, reduces the barcode sequencing cost to < 10 cents, allows fast turnaround from specimen sequence by using portable...
DNA obtained from invertebrates (iDNA) can be metabarcoded in order to survey vertebrate communities. However, little attention has been paid the interaction between invertebrate and species. Here, we tested for specialization by sampling dung carrion fly community of a swamp forest remnant along disturbance gradient (10 sites: 80-310 m road). Approximately, 60% baited 407 flies yielded 294 identifications based on two COI fragments 16S. A bipartite network analysis found no statistically...
Millions of species remain undescribed, requiring molecular and morphological diagnoses. However, no efficient software reliably derives tests -state-specific contrastive- UITOTO addresses this gap by identifying, testing, visualizing Diagnostic Molecular Combinations (DMCs). The uses a weighted random sampling algorithm based on the Jaccard Index for building candidate DMCs. It then selects DMCs with highest specificity stability, meeting user-defined thresholds exclusive character states....
Abstract Species extinctions increase at a global scale; therefore, rapid inventorying of our planet's biodiversity is becoming more and important. As insects represent the highest portion fauna play key ecological roles, it pressing need to investigate their accelerate species discovery, especially for understudied insect groups, also known as “dark taxa.” Phoridae (Diptera) are great example taxon,” in particular genus Megaselia Rondani. The use integrative methodologies best approach face...
Abstract Many phoretic relationships between arthropods are understudied because of taxonomic impediments. We here illustrate for avian lice riding on hippoboscid flies how new natural history data can be acquired quickly with modern and cost‐effective barcoding techniques. Most host‐specific, but some arrive hosts by hitchhiking that feed bird blood. Our summary the literature yielded 254 published records which we show to belong two large 13 small interaction networks birds, flies, lice....
Abstract Background DNA barcodes are a useful tool for discovering, understanding, and monitoring biodiversity which critical tasks at time of rapid loss. However, widespread adoption requires cost-effective simple barcoding methods. We here present workflow that satisfies these conditions. It was developed via “innovation through subtraction” thus minimal lab equipment, can be learned within days, reduces the barcode sequencing cost to <10 cents, allows fast turnaround from specimen...
Abstract Background Zoology’s dark matter comprises hyperdiverse, poorly known taxa that are numerically dominant but largely unstudied, even in temperate regions where charismatic well understood. It is everywhere, high diversity, abundance, and small size have historically stymied its study. We demonstrate how entomological can be elucidated using high-throughput DNA barcoding (“megabarcoding”). reveal the abundance diversity of scuttle flies (Diptera: Phoridae) Sweden 31,800 specimens...
ABSTRACT Metabarcoding of vertebrate DNA obtained from invertebrates (iDNA) has been used to survey communities, but we here show that it can also be study species interactions between and vertebrates in a spatial context. We sampled the dung carrion fly community swamp forest remnant along disturbance gradient (10 sites: 80-310m road). Approximately, 60% baited 407 flies yield 294 identifications based on two COI fragments 16S sequenced with Illumina and/or MinION. A bipartite network...
Abstract Many phoretic relationships between insects are understudied because of taxonomic impediments. We here illustrate for avian lice riding on hippoboscid flies how new natural history data can be acquired quickly with NGS barcoding. Most host-specific, but some arrive hosts by that feed bird blood. Our summary the literature yielded 254 published records which we show to belong two large and 13 small interaction networks birds, flies, lice. In order generate records, then developed a...