A5060910915- Periodontal Regeneration and Treatments
- Antifungal resistance and susceptibility
- Tendon Structure and Treatment
- Fungal Infections and Studies
- Parasitic Infections and Diagnostics
- Pneumocystis jirovecii pneumonia detection and treatment
- Platelet Disorders and Treatments
- Neutropenia and Cancer Infections
- Pneumonia and Respiratory Infections
- Amoebic Infections and Treatments
- Shoulder Injury and Treatment
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Venous Thromboembolism Diagnosis and Management
- Hemostasis and retained surgical items
- Osteoarthritis Treatment and Mechanisms
- Vascular anomalies and interventions
- Antimicrobial Resistance in Staphylococcus
- Antibiotics Pharmacokinetics and Efficacy
- Surgical Sutures and Adhesives
- Helminth infection and control
- Infections and bacterial resistance
- Infectious Encephalopathies and Encephalitis
- Probiotics and Fermented Foods
- Toxoplasma gondii Research Studies
Hospital Universitario Ramón y Cajal
2003-2020
University of Caldas
2000-2018
Hospital General Universitario Gregorio Marañón
2013
Hospital Universitario 12 De Octubre
2009
Instituto Cajal
2004
Central University Hospital of Asturias
1997
Anderson Hospital
1985-1987
The University of Texas at Austin
1986
University of Puerto Rico, Medical Sciences Campus
1985
The University of Texas System
1985
Abstract Background There is no information on the effects of breed, gender and age cellular content growth factor (GF) release from equine pure-platelet rich plasma (P-PRP) gel (P-PRG). The objectives this study were: 1) to compare composition P-PRP with whole blood platelet poor (PPP); 2) concentration transforming GF beta 1 (TGF-β ) derived isoform BB (PDGF-BB) between treated non-ionic detergent (P-PRP+NID), P-PRG (activated calcium gluconate -CG-), PPP+NID, PPP (PPG), and; 3) evaluate...
Invasive aspergillosis (IA) is an important cause of mortality in liver transplant patients. Clinical and microbiological diagnosis difficult, it frequently achieved only after autopsy. Early antifungal therapy could improve the survival these patients.A retrospective case-control study IA recipients (OLT) was performed to determine value detection galactomannan Aspergillus antigen serum using a sandwich-ELISA test (Platelia, Sanofi Diagnostic Pasteur). Stored frozen specimens obtained...
Imipenem-cilastatin was used to treat 79 febrile episodes in 71 cancer patients, most of whom had neutropenia. The overall response rate 67%, and 76% the 45 documented infections responded. rates for septicemias pneumonias were 62%, respectively. Only 1 17 caused by gram-negative bacilli failed respond this therapy. common side effects skin rash, nausea, diarrhea. Eight superinfections detected during
Aspergillus galactomannan (GM) antigenemia is an early marker of invasive aspergillosis (IA), but may yield false-positive results. A prospective study, testing GM periodically in serum samples liver transplant recipients, was performed.An index more than or equal to 0.5 were considered positive. Positive from patients without any other criteria proven probable IA as false-positive. The test performed weekly during the first month after transplantation.Three developed IA. In total, 414 85...
There is a lack information on the effects of most commonly used anticoagulants for equine platelet rich plasmas (PRPs) elaboration cell counts and growth factor release from gels (PRGs). The aims this study were 1) to compare sodium citrate (SC), acid dextrose solution A (ACD-A) ACD-B (PLT), leukocyte (WBC) some parameters associated activation including mean volume (MPV) distribution width (PDW) between whole blood, pure PRP (P-PRP) platelet-poor plasma (PPP); 2) transforming beta 1...
Platelet-rich plasma (PRP) preparations are a common treatment in equine osteoarthritis (OA). However, there controversies regarding the ideal concentration of platelets and leukocytes these biological substances necessary to induce an adequate anti-inflammatory anabolic response articular cartilage. The aims were study influence leukocyte- platelet-rich gel (L-PRG) pure (P-PRG) supernatants on histological changes cartilage, degree chondrocyte apoptosis, production hyaluronan (HA) gene...
Leukocyte-reduced platelet-rich plasma (LR-PRP) is a therapy for tendinopathy of the Achilles tendon (TAT); however, there scarce information regarding LR-PRP effects in rabbit models TAT. We compared, at 4 and 12 weeks (w), placebo (PBS) on ultrasonography, histology relative gene expression collagen types I (COL1A1) III (COL3A1) vascular endothelial growth factor (VEGF) 24 rabbits with TAT induced by collagenase. The (treated both treatments) were euthanatised after either or w. A healthy...
IntroductionA possible increase in Candida resistance, especially glabrata, has been speculated according to poor diffusion of echinocandins peritoneal fluid.Materials/methodsPeritoneal and serum concentrations caspofungin, micafungin anidulafungin were analysed surgical patients with suspected candida peritonitis. After 4 days starting therapy, samples (through drainage) obtained at baseline, 1, 6, 12 24 h drug administration. Micafungin determined using high-performance liquid...
Platelet-rich plasma (PRP) preparations are a common treatment in osteoarthritis (OA) and inflammatory synovitis. However, there is ambiguity regarding the ideal concentration of leukocytes platelets these necessary to induce an adequate anti-inflammatory anabolic response joint tissues, such as synovial membrane. This research aimed study, normal membrane explants (SME) challenged with lipopolysaccharide (LPS), temporal effects (at 48 96h) leukocyte- platelet-rich gel (L-PRG) pure (P-PRG)...
Summary Objectives: 1) To evaluate the bacteriostatic in vitro effect of pure platelet-rich plasma (P-PRP), gel (P-PRG), leukocyte-poor (LPG), platelet-poor (PPP), and heat inactivated (IP) against methicillin-sensitive Staphylococcus aureus (MSSA) over a period 24 hours. 2) determine degradation platelet factor-4 (PF-4), transforming growth factor beta 1 (TGF-β1), platelet-derived isoform BB (PDGF-BB) these equine blood components. 3) establish correlations between leukocyte counts, PF-4...
Summary Objective: To compare five activation methods in equine platelet-rich plasma (PRP) by determination of platelet-derived growth factor BB (PDGF-BB) and transforming beta 1 (TGF-β1) concentrations gel (PRG) supernatants. Methods: Platelet-rich from 20 horses was activated calcium chloride (CC), gluconate (CG), bovine thrombin (BT), their combinations, BTCC BTCG. Both PRG supernatants were measured ELISA compared with platelet ly-sates (PL) over time. Results: Growth significantly lower...
Imipenem/cilastatin was administered during 153 febrile episodes occurring in cancer patients and the response rate 68%. Considering only documented infections 71%. Patients who received imipenem as initial therapy had a higher than it after failing other antibiotics (77% versus 68%). The overall rates for septicentias pneumonias were 75% 58%. Among 57 gram-negative 77% responded, but substantially if used (94% 69%). poorest observed when given secondary Pseudomonas (50%), most of these...
The aims were (1) to evaluate the bacteriostatic effect of platelet-rich plasma (PRP), gel (PRG), leukocyte-poor (LPP), (LPG), plasma, and heat-inactivated (IP) on both methicillin-sensitive Staphylococcus aureus (MSSA) methicillin-resistant (MRSA) over a period 24 h; (2) determine compare concentrations degradation time platelet factor 4 (PF-4), transforming growth beta 1 (TGF- β ), platelet-derived isoform BB (PDGF-BB); (3) identify any correlations between MSSA MRSA either cellular, PF-4,...
There are not reported regarding the protocols for obtaining platelet concentrates (PC) in cats medical purposes. The objectives of this study were: 1) to describe a manual method producing two kinds PC (PC-A and PC-B), 2) cellular population PC, 3) measure compare effect calcium gluconate (CG) bovine thrombin (BT) on temporal release transforming growth factor beta 1 (TGF-β1) platelet-derived type BB (PDGF-BB) at 3 12 hours post-activation 4) establish correlations between both PCs...
Summary Reasons for performing study: There is a growing interest in the use of autologous platelet concentrates (PCs) as treatment chronic musculoskeletal diseases horses. However, there no information on risk bacterial contamination during their preparation. Objectives: To: 1) assess equine PCs obtained by tube method under 3 technical conditions: a) laminar flow cabinet, clean laboratory environment both with (b) and without (c) Bunsen burner; 2) identify critical points process...
The aim of this study was to evaluate the release pro- and anti-inflammatory as well anabolic mediators stimulated by a leukocyte-reduced platelet-rich gel supernatant (Lr-PRGS) plasma (Lr-PL) at two concentrations (25 50%) on normal equine suspensory ligament explants (SLEs) tendon (TEs). SLEs TEs from six horses were independently incubated for 48 h with Lr-PRGS Lr-PL 25 50%, respectively. Samples collected tissues 1 h, which employed ELISA determination interleukin beta (IL-1β), tumor...
Abstract Multiplex quantitative real-time PCR (MRT-PCR) using blood can improve the diagnosis of intra-abdominal candidiasis (IAC). We prospectively studied 39 patients with suspected IAC in absence previous antifungal therapy. Blood cultures, MRT-PCR, and β-D-glucan (BDG) serum were performed all patients. was defined according to 2013 European Consensus criteria. For probes targeted ITS1 or ITS2 regions ribosomal DNA. Candidaemia confirmed only four (10%), criteria present 17 (43.6%). The...
Platelet-rich plasma (PRP) preparations are used in horses with osteoarthritis (OA). However, some controversies remain regarding the ideal concentration of platelets and leukocytes to produce an adequate anti-inflammatory anabolic response synovial membrane. The aims this study were influence leukoconcentrated platelet-rich gel (Lc-PRG) leukoreduced (Lr-PRG) supernatants on quantitative expression proinflammatory genes equine membrane explants (SMEs) challenged lipopolysaccharide (LPS)....
In 1997, a surveillance program to assess the main bacterial pathogens associated with acute diarrheal disease was initiated by Microbiology Group of lnstituto Nacional de Salud in collaboration Public Health Laboratories (PHL) country and PAHO. The objective identify serotypes antimicrobial resistance patterns Salmonella spp., Shigella spp, Vibrio cholerae 01 isolates. Twenty two PHL participated total 976 isolates from 1997 1999; 96% were clinical 4% food 34% 23% 42% V. 01. From isolates,...