A5014602162- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Protease and Inhibitor Mechanisms
- Developmental Biology and Gene Regulation
- Axon Guidance and Neuronal Signaling
- Wnt/β-catenin signaling in development and cancer
- 3D Printing in Biomedical Research
- Cancer-related gene regulation
- Proteoglycans and glycosaminoglycans research
- RNA Research and Splicing
- Signaling Pathways in Disease
- RNA Interference and Gene Delivery
- Angiogenesis and VEGF in Cancer
- Zebrafish Biomedical Research Applications
- Silk-based biomaterials and applications
- Microtubule and mitosis dynamics
- Fibroblast Growth Factor Research
- Cancer Cells and Metastasis
- Skin and Cellular Biology Research
- Congenital heart defects research
- Mathematical Biology Tumor Growth
- Biochemical and Structural Characterization
- Chemical Synthesis and Analysis
- Caveolin-1 and cellular processes
- Erythrocyte Function and Pathophysiology
University of Virginia
2012-2025
University of Virginia Health System
2001-2018
Czech Academy of Sciences, Institute of Microbiology
2012
Morpho (United States)
2004-2008
Institute of Cell Biology
2001
Laboratoire de Biologie du Développement
1995
Centre National de la Recherche Scientifique
1995
Brigham and Women's Hospital
1993
University of Virginia Medical Center
1992
Massachusetts Institute of Technology
1986-1990
The connexin 43 (Cx43) hemichannel (HC) in the mechanosensory osteocytes is a major portal for release of factors responsible anabolic effects mechanical loading on bone formation and remodeling. However, little known about how Cx43 molecule responds to stimulation leading opening HC. Here, we demonstrate that integrin α5β1 interacts directly with this interaction required stimulation-induced Direct perturbation via magnetic beads or conformational activation leads HC, role independent its...
The mechanistic links between mechanical forces and bioenergetics remain elusive. We report an increase in mitochondrial membrane potential (MMP) along the leading row of collectively migrating Xenopus laevis mesendoderm cells at sites where fibronectin- a5b1 integrin substrate traction stresses are greatest. Real-time metabolic analyses reveal integrin-dependent increases respiration efficiency on fibronectin substrates. Elevation activity is reduced following pharmacologic inhibition focal...
Fibronectin (FN) is reported to be important for early morphogenetic movements in a variety of vertebrate embryos, but the cellular basis this requirement unclear. We have used confocal and digital time-lapse microscopy analyze cell behaviors Xenopus gastrulae injected with monoclonal antibodies directed against central cell-binding domain fibronectin. Among defects observed disruption fibronectin matrix assembly, resulting failure radial intercalation movements, which are required...
A new member of the thrombospondin gene family, designated thrombospondin-4, has been identified in Xenopus laevis genome. The predicted amino acid sequence indicates that protein is similar to other members this family structure type 3 repeats and COOH-terminal domain. Thrombospondin-4 contains four 2 lacks 1 are found thrombospondin-1 2. amino-terminal domain thrombospondin-4 no significant homology with or proteins database. RNAse protection analysis establishes initial expression...
Fibronectin, a major component of the extracellular matrix is critical for processes cell traction and motility. Whole-mount confocal imaging three-dimensional architecture used to describe dynamic assembly remodeling fibronectin fibrils during gastrulation neurulation in early frog embryo. As previously reported, first appear under prospective ectoderm. We here evidence regulated along somitic mesoderm/endoderm boundary as well at notochord/somitic mesoderm clearing from dorsal ventral...
We report the sequences of cDNA clones for two different integrin beta subunits isolated from a Xenopus laevis neurula library. mRNAs corresponding to both genes are first detected at gastrulation. show that these very highly related (98% identity in amino acid sequence) and probably arose time tetraploidization X. genome around 50 million years ago. Comparison with those various other vertebrate subunit establishes all species analyzed date contain conserved (beta 1). The interspecies...
Abstract Two distinct cadherin cDNA clones of Xenopus laevis were isolated from a stage 17 embryo library. Analysis the complete deduced amino acid sequences indicated that one these molecules is closely homologous to chicken and mouse N-cadherin, while other displays comparable homology both E- P-cadherins was thus denoted EP-cadherin. This molecule has an apparent relative molecular mass 125 ×103 (compared approx. 138 or 140 x103 E-cadherin N-cadherins, respectively). Northern Western blot...
Abstract Exposure of isolated Xenopus animal pole ectoderm to the XTC mesoderm-inducing factor (XTC-MIF) causes tissue undergo gastrulation-like movements. In this paper, we take advantage observation investigate control various aspects gastrulation in Xenopus. Blastomeres derived from induced regions are able, like marginal zone cells, but unlike blastomeres, spread and migrate on a fibronectin-coated surface. Dispersed cells also able respond XTC-MIF way; is one few mesoderm-specific...
During Xenopus laevis gastrulation, the basic body plan of embryo is generated by movement marginal zone cells blastula into blastocoel cavity. This morphogenetic process involves cell adhesion to extracellular matrix protein fibronectin (FN). Regions FN required for attachment and migration involuting (IMZ) were analyzed in vitro using fusion substrates. IMZ mediated Arg-Gly-Asp (RGD) sequence located type III-10 repeat Pro-Pro-Arg-Arg-Ala-Arg (PPRRAR) III-13 Hep II domain. spread migrate...
ADAMs are membrane-anchored proteases that regulate cell behavior by proteolytically modifying the surface and ECM. Like other proteases, contain candidate “adhesive” domains downstream of their metalloprotease domains. The mechanism which utilize these putative adhesive to protease function in vivo is not well understood. We address this important question analyzing relative contributions extracellular (disintegrin, cysteine rich, EGF-like repeat) ADAM13 during Xenopus laevis development....