// Han-Ching Tseng 1, * , Keiichi Kanayama 2, 3, Kawaljit Kaur So-Hyun Park 1 Sil 2 Anna Kozlowska 4 Shuting Sun 5 Charles E. McKenna Ichiro Nishimura Anahid Jewett Division of Oral Biology and Medicine, The Jane Jerry Weintraub Center for Reconstructive Biotechnology, UCLA School Dentistry, Los Angeles, CA, USA Advanced Prosthodontics, 3 Department Periodontology, Asahi University Gifu, Japan Tumor Immunology, Poznan Medical Sciences Poznan, Poland Chemistry, Southern California, These...

Natural Killer (NK) cells target oral, pancreatic, lung, breast, glioblastoma and melanoma stem-like/poorly differentiated tumors. Differentiation of the abovementioned tumors with supernatants from split-anergized NK decreases their susceptibility to cells, but increases sensitivity cisplatin (CDDP)-mediated cell death. Breast tumor CD44 knockdown display enhanced cell-mediated lysis, potentially due decreased differentiation. We also demonstrate that sulindac, a non-steroidal...

Natural killer (NK) cells are known to target cancer stem and undifferentiated tumors. In this paper, we provide a novel strategy for expanding large numbers of super-charged NK with significant potential lyse differentiate demonstrate the differences in dynamics cell expansion between healthy donors patients. Decline cytotoxicity lower interferon (IFN)-γ secretion by osteoclast (OC)-expanded from patients correlates faster residual contaminating T within purified cells, whereas donors' OCs...

Our previous reports demonstrated that the magnitude of NK cell mediated cytotoxicity correlated directly with stage and levels differentiation tumor cells. In addition, we have shown previously activated cells induce growth inhibition cancer through induction resulting in resistance to secreted cytokines, as well direct NK-tumor contact. this report show comparison IL-2+anti-CD16mAb treated cells, activation by probiotic bacteria sAJ2 combination IL-2 anti-CD16mAb substantially decreases...

Therapeutic role of NK cells in solid tumors was challenged previously even though their hematological malignancies has clearly been established. Furthermore, functions and numbers are greatly suppressed oral cancer patients necessitating effective future immunotherapeutic strategies to aid the control disease. The humanized-BLT (hu-BLT) mice were used implant stem-like/undifferentiated study super-charged with without feeding AJ2 probiotic bacteria. Implanted CSC/undifferentiated resected...

Osteonecrosis of the jaw (ONJ), an uncommon co-morbidity in patients treated with bisphosphonates (BP), occurs segment jawbone interfacing oral mucosa. This study aimed to investigate a role mucosal barrier γδ T cells pathogenesis ONJ. Female C57Bl/6J (B6) mice received bolus zoledronate intravenous injection (ZOL, 540 μg/kg), and their maxillary left first molars were extracted 1 week later. ZOL-treated (WT ZOL) delayed wound healing patent open wounds 4 weeks after tooth extraction...

Background and Aims: We have previously demonstrated that the stage of differentiation tumors has profound effect on function NK cells, stem-like/poorly differentiated were preferentially targeted by cells. Therefore, in this study we determined role super-charged cells immune mobilization, lysis, stem-like/undifferentiated implanted pancreas humanized-BLT (hu-BLT) mice fed with or without AJ2 probiotics. The phenotype, growth rate metastatic potential pancreatic (NK-differentiated) patient...

Introduction and methods In this study we report that sequential treatment of supercharged NK (sNK) cells with either chemotherapeutic drugs or check-point inhibitors eliminate both poorly differentiated well tumors in-vivo in humanized-BLT mice. Background results sNK were found to be a unique population activated genetic, proteomic, functional attributes are very different from primary untreated IL-2 treated cells. Furthermore, NK-supernatant well-differentiated oral pancreatic tumor cell...

We demonstrate that natural killer (NK) cells induce a higher cytotoxicity against lung cancer stem-like (hA549) compared to differentiated cell lines (H292). The supernatants from split-anergized NK (IL-2 and anti-CD16 mAb-treated cells) induced differentiation in hA549. Differentiated line (H292) hA549 expressed reduced cell-mediated but sensitivity chemotherapeutic drugs. This finding validated our previous reports demonstrating the levels of tumor killing by drugs correlate directly...

Abstract Background Antibiotic use is commonly associated with disruption of the healthy human gut microbiota (termed dysbiosis). Dysbiosis can diminish colonisation resistance and increase susceptibility to Clostridioides difficile infection (CDI). Use pathogen-specific anti-staphylococcal afabicin was not dysbiosis in animals volunteers (Nowakowska et al, 2023). Here, we assessed whether this microbiota-sparing property would limit CDI a clinically reflective vitro model. Methods A...

NK cells have traditionally been classified as effectors of innate immunity, even though they also exhibit some features adaptive immunity such memory. contribute to the lysis and growth inhibition cancer, mediating direct cytotoxicity antibody-dependent cellular (ADCC) regulating functions other immune cells, respectively. regulate function via release inflammatory cytokines chemokines. Currently, cell therapeutics in oral cancer less efficient due several limitations, follows: (a) lower...

Abstract Osteoclasts (OCs) and dendritic cells (DCs) induce expansion functional activation of NK T cells. When comparing OCs with DC-induced in cells, induced significantly higher cell as compared to DCs, either from healthy individuals or those obtained cancer patients. However, no differences could be seen the levels activated by selectively expanded CD8 + whereas DCs CD4 In addition, both allogeneic autologous similar Together, these findings highlighted essential roles expanding...

Natural killer (NK) cells are known for their cytotoxic and cytokine secretion capabilities. The balance of activating inhibitory receptors on surface regulates NK cell function survival. However, it is not fully understood how aging may modulate the levels ultimately affecting interaction with other immune cells, especially those to activate expand cells. Here, we report decreased cells' receptors, function, in aged donors (75-85 years) as compared younger (21-25 years). We used our...

Objective To demonstrate the significance of supercharged natural killer (sNK) cells to target aggressive gynecological tumours. Methods and analysis We used cell cultures peripheral blood-derived mononuclear (PBMCs) purified NK alone in presence MHC-class gene expression assessments ovarian tumours were performed using set enrichment (GSEA). Secretion levels cytokines PBMCs determined ELISA scRNA seq analysis, respectively. A flow cytometer was for surface marker analysis. 51 Cr eSight...

Tumor cells are known to upregulate major histocompatibility complex-class I chain related proteins A and B (MICA/B) expression under stress conditions or due radiation exposure. However, it is not clear whether there specific stages of cellular maturation in which these ligands upregulated the natural killer (NK) differentially target tumors direct cytotoxicity antibody-dependent cell (ADCC). We used freshly isolated primary osteoclast (OCs)-expanded NK determine degree ADCC using...

High-grade serous ovarian cancers (HGSOCs) likely consist of poorly differentiated stem-like cells (PDSLCs) and tumor cells. Conventional therapeutics are incapable completely eradicating PDSLCs, contributing to disease progression relapse. Primary NK known effectively lyse but they exhibit low or minimal cytotoxic potential against well-differentiated tumors. We have introduced discussed the characteristics super-charged (sNK) in this review. sNK cells, comparison primary a significantly...

The combined/synergistic effect of genetic mutation KRAS in the pancreas and obesity, a life-style factor on suppression NK cells at pre-neoplastic stage pancreatic cancer is not yet investigated subject this report. Obese mice with (KC) fed high fat calorie diet (HFCD) exhibit severe deficiencies cell expansion function cancer. Decreased cell-mediated cytotoxicity observed peripheral blood, spleen, peri-pancreatic-adipose tissue obese KC mice, whereas bone marrow an increased when compared...

Abstract Osteoclasts (OCs) and much less dendritic cells (DCs) induce significant expansion functional activation of NK cells, furthermore, the OC-expanded preferentially increase CD8+ T by targeting CD4+ cells. When autologous OCs were used to expand patient lower percentages expanded decreased numbers functions could be observed, addition allogeneic healthy increased patients’ function. Mechanistically, found lyse but not suggesting potential selection before their OC activated In...

Hepatocellular carcinoma (HCC) is the most common primary liver cancer and fourth-leading cause of all cancer-related deaths around world. Liver transplantation, surgery, local ablation are curative therapies for early-stage HCC. However, post-treatment outcomes can vary based on histopathologic stage. Poorly-differentiated HCC associated with higher rates tumor progression lower overall survival compared to well-differentiated after therapy. In this study, we aimed characterize stem cell...