A5073245889- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Nail Diseases and Treatments
- Oral microbiology and periodontitis research
- Plant Pathogens and Fungal Diseases
- Infectious Diseases and Mycology
- Oral and gingival health research
- Plant Disease Resistance and Genetics
- Actinomycetales infections and treatment
- Phytoplasmas and Hemiptera pathogens
- Mycotoxins in Agriculture and Food
- Pineapple and bromelain studies
Fundação Oswaldo Cruz
2013-2018
Instituto de Saúde
2018
Instituto Evandro Chagas
2014-2017
Universidade Federal Fluminense
2013
ObjectivesThe emerging fungal pathogens comprising the Candida haemulonii complex (Candida haemulonii, var. vulnera and duobushaemulonii) are notable for their antifungal resistance. Twelve isolates with phenotypic similarity to C. were recovered from patients in Brazilian hospitals. Here we aimed identify these by a molecular approach, using current classification of this complex, evaluate susceptibility profiles.
The production of virulence attributes in three reference strains and 11 clinical isolates primarily identified as Candida parapsilosis was evaluated. Morphological phenotypical tests were not able to discriminate among the species C. complex; consequently, molecular methods applied solve this task. After employing polymerase chain reaction-based methods, nine sensu stricto two orthopsilosis. Protease, catalase, hemolysin produced by all 14 strains, while 92.9% 78.6% secreted, respectively,...
Nosocomial fungal bloodstream infections (BSI) are increasing significantly in hospitalized patients and Candida parapsilosis has emerged as an important pathogen responsible for numerous outbreaks. The objective of this study was to evaluate C. sensu lato infection scenarios, regarding species distribution strain relatedness. One hundred isolates derived from blood cultures catheter tips were analysed by multiplex microsatellite typing sequencing D1/D2 regions the ribosomal DNA. Our results...
This study evaluated the antifungal susceptibility profile and production of potential virulence attributes in a clinical strain Candida nivariensis for first time Brazil, as identified by sequencing internal transcribed spacer (ITS)1-5.8S-ITS2 region D1/D2 domains 28S rDNA. For comparative purposes, tests were also performed with reference strains. All strains presented low planktonic minimal inhibitory concentrations (PMICs) to amphotericin B (AMB), caspofungin (CAS), voriconazole....
Candida glabrata is a facultative intracellular opportunistic fungal pathogen in human infections. Several virulence-associated attributes are involved its pathogenesis, host-pathogen interactions, modulation of host immune defenses, and regulation antifungal drug resistance. This study evaluated the vitro susceptibility profile to five agents, production seven hydrolytic enzymes related virulence, relationship between these phenotypes 91 clinical strains C. . All were susceptible...
Currently, it is accepted that there are three species were formerly grouped under Candida parapsilosis: C. para- psilosis sensu stricto, orthopsilosis, andCandida metapsilosis. In fact, the antifungal susceptibility profiles and distinct virulence attributes demonstrate differences in these nosocomial pathogens. An accurate, fast, economical identification of fungal has been main goal mycology. present study, we searched sequences available GenBank database order to identify complete...
Since the description of Candida orthopsilosis and C. metapsilosis in 2005, several methods have been proposed to identify differentiate these species from parapsilosis sensu stricto. Species-specific uniplex polymerase chain reaction (PCR) was performed compared with sequencing D1/D2 region LSU 28S rDNA gene, microsatellite typing stricto, PCR-restriction fragment length polymorphism patterns ITS1-5.8S-ITS2 gene. There agreement between results testing 98 clinical isolates four PCR-based...
Histoplasmosis is a systemic mycosis infection caused by Histoplasma capsulatum, heterothallic ascomycete. The sexual reproduction of this fungus regulated the mating type (MAT1) locus that contains MAT1-1 and MAT1-2 idiomorphs, which were identified uniplex polymerase chain reaction (PCR). This study aimed to optimise single-step multiplex PCR for accurate detection distinct types H. capsulatum. Among 26 isolates tested, 20 had genotype, while six showed in agreement with results. These...