A5100354206- Advanced Fluorescence Microscopy Techniques
- Nuclear Receptors and Signaling
- Molecular Sensors and Ion Detection
- Macrophage Migration Inhibitory Factor
- Neuroscience and Neuropharmacology Research
- Sulfur Compounds in Biology
- Photoreceptor and optogenetics research
- Redox biology and oxidative stress
- Nicotinic Acetylcholine Receptors Study
- Eicosanoids and Hypertension Pharmacology
- Neurobiology and Insect Physiology Research
- Ion channel regulation and function
- Electrochemical sensors and biosensors
- Advanced biosensing and bioanalysis techniques
- Ocular and Laser Science Research
- Synthesis of Tetrazole Derivatives
- Advanced Biosensing Techniques and Applications
- Ion Channels and Receptors
- Hydrogen's biological and therapeutic effects
- Spectroscopy Techniques in Biomedical and Chemical Research
- Insect Utilization and Effects
- Gene Regulatory Network Analysis
- Asthma and respiratory diseases
- Cellular transport and secretion
- Advanced Optical Sensing Technologies
University of Science and Technology of China
2024
Zhengzhou University
2023
Shanghai Jiao Tong University
2023
University of Virginia
2018-2021
University of Virginia Cancer Center
2020
University of California, Riverside
2018
Soochow University
2015-2017
Chinese Academy of Agricultural Sciences
2017
Institute of Plant Protection
2017
Huaihua University
2008
Capacitative Ca 2+ entry (CCE) through store-operated (SOC) channels plays an important role in returning to the sarcoplasmic reticulum (SR) and regulating cytosolic free concentration ([Ca ] cyt ). A rise [Ca sufficient SR are required for pulmonary artery smooth muscle cell (PASMC) proliferation. We tested hypothesis that platelet-derived growth factor (PDGF)-mediated PASMC involves upregulation of c-Jun TRPC6, a transient receptor potential cation channel. In rat PASMC, PDGF (10 ng/ml...
SO2 and polarity are important microenvironmental parameters in cells, which closely related to physiological activities organisms. The intracellular levels of abnormal inflammatory models. To this end, a novel near-infrared fluorescent probe BTHP that can simultaneously detect was studied. sensitively change with emission peak from 677 818 nm. also fluorescence red green. After addition SO2, the intensity ratio I517/I768 increased by about 33.6 times. determine bisulfite single crystal rock...
Melatonin shows significant protective effects in Alzheimer's disease (AD) models vitro and vivo; these are related to its function as an antioxidant. The source of reactive oxygen species (ROS) generation the AD brain is primarily amyloid-beta (Abeta)- activated microglial nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. However, melatonin on activation NADPH oxidase remain unclear. In present study, cultures microglia were incubated presence fibrillar Abeta(1-42), which induces...
Hydrogen sulfide (H2S) is an important gasotransmitter. Although a large number of fluorescent probes for cellular H2S have been reported, only few can detect in mitochondria, organelle connecting with mitochondrial function and metabolic pathways. We hereby describe novel near-infrared probe, nimazide, by introducing sulfonyl azide to the core structure QSY-21 dark quencher. Nimazide responded quickly H2S, resulting robust fluorescence turn-off changes. This conversion displayed high...
The multi-subunit homotypic fusion and vacuole protein sorting (HOPS) membrane-tethering complex is required for autophagosome-lysosome in mammals, yet reconstituting the mammalian HOPS remains a challenge. Here we propose "hook-up" model assembly, which requires two sub-complexes docking on membranes via membrane-associated Rabs. We identify Rab39A as key small GTPase that recruits onto autophagic vesicles. Proper pairing with Rab2 enables assembly between proteoliposomes its tethering...
As an important gasotransmitter, hydrogen sulfide (H2S) plays crucial roles in cell signaling. Incorporation of p-azidophenylalanine (pAzF) into fluorescent proteins (FPs) via genetic code expansion has been a successful strategy developing intensity-based, genetically encoded biosensors for H2S. To extend this ratiometric measurement which eliminates many detection uncertainties self-calibration at two wavelengths, we modified the chromophore circularly permutated, superfolder green protein...
Nur77, an orphan member of the nuclear receptor superfamily, has been implicated in regulation inflammation. However, vivo function Nur77 remains largely unexplored. In current study, we investigated role inflammation and immunity mice. We found that elderly 8-month-old Nur77-deficient mice (Nur77−/−) developed systemic Compared to wild-type (WT) (Nur77+/+), Nur77−/− showed splenomegaly, severe infiltration inflammatory cells several organs including liver, lung, spleen kidney, increased...
Nur77, a key member of the NR4A receptor subfamily, is involved in regulation inflammation and immunity. However, vivo regulatory roles Nur77 sepsis mechanisms remains largely elusive. In this study, we used Nur77-deficient (Nur77(-/-)) mice investigated function sepsis.Compared to wild-type (Nur77(+/+)) mice, Nur77(-/-) are more susceptible LPS-induced acute liver inflammation. Mechanistically, observed that can interact with TRAF6, crucial adaptor molecule Toll-like receptor-interleukin 1...
As an essential element for living organisms, zinc (Zn2+) exerts its biological functions both intracellularly and extracellularly. Previous studies have reported a number of genetically encoded Zn2+ indicators (GEZIs), which been widely used to monitor in the cytosol intracellular organelles. However, it is challenging localize existing GEZIs extracellular space detect secreted Zn2+. Herein, we report two photostable, green fluorescent protein (GFP) based indicators, ZIBG1 ZIBG2, respond...
The green lacewing, Chrysopa pallens Rambur, is one of the most important natural predators because its extensive spectrum prey and wide distribution. However, what we know about nutritional reproductive physiology this species very scarce.By cDNA amplification Illumina short-read sequencing, analyzed transcriptomes C. female adult under starved fed conditions. In total, 71236 unigenes were obtained with an average length 833 bp. Four vitellogenins, three insulin-like peptides two insulin...
The authors have withdrawn their manuscript whilst they perform additional experiments to test some of conclusions. Therefore, the do not wish this work be cited as reference for project. If you any questions, please contact corresponding author. Details Regrettably, we, and several other researchers in author’s lab involved original study, been unable reproduce reported key results under conditions originally described. We recently used pDisplay-G-GESS-M plasmid transfect HEK 293T cells...
Nur77, an orphan member of the nuclear receptor superfamily, plays critical roles in inflammation and immunity. However, role Nur77 tumor microenvironment remains elusive. Results showed that deletion strikingly enhanced metastasis compared to WT mice. Additionally, conditioned media derived from Nur77+/+ peritoneal macrophages (CM1), Nur77-/- (CM2) significantly promoted EMT cancer cells, greatly migratory invasive abilities cells. Moreover, studies using TNF-α blocking antibody...
Summary Peroxynitrite is a highly reactive nitrogen species (RNS) that plays critical roles in signal transduction, stress response, and numerous human diseases. Advanced molecular tools permit the selective, sensitive, non-invasive detection of peroxynitrite essential for understanding its pathophysiological functions. Here, we present pnGFP-Ultra, high performance, reaction-based, genetically encodable biosensor imaging live cells. pnGFP-Ultra features p -boronophenylalanine-modified...