A5037576424- Malaria Research and Control
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- COVID-19 diagnosis using AI
- Mosquito-borne diseases and control
- Extracellular vesicles in disease
- Complement system in diseases
- Viral Infections and Outbreaks Research
- Parasites and Host Interactions
- Biosensors and Analytical Detection
- Amino Acid Enzymes and Metabolism
- Viral gastroenteritis research and epidemiology
- Invertebrate Immune Response Mechanisms
- Scientific Computing and Data Management
- Single-cell and spatial transcriptomics
- MicroRNA in disease regulation
- Evolution and Genetic Dynamics
- RNA modifications and cancer
- Simulation Techniques and Applications
- Epilepsy research and treatment
Kenya Medical Research Institute
2020-2024
Wellcome Trust
2020-2023
Jomo Kenyatta University of Agriculture and Technology
2020-2021
The malaria parasite life cycle includes asexual replication in human blood, with a proportion of parasites differentiating to gametocytes required for transmission mosquitoes. Commitment differentiate into gametocytes, which is marked by activation the transcription factor ap2-g , known be influenced host factors but comprehensive model remains uncertain. Here, we analyze data from 828 children Kilifi, Kenya severe, uncomplicated, and asymptomatic infection over 18 years falling...
Plasmodium falciparum secretes extracellular vesicles (PfEVs) that contain parasite-derived RNA. However, the significance of secreted RNA remains unexplored. Here, we compare and intracellular from asexual cultures six P. lines. We find secretion via is not only periodic throughout intraerythrocytic developmental cycle but also highly conserved across isolates. further demonstrate phases are discernibly shifted compared to those within secreting whole parasite. Finally, transcripts genes...
Abstract Background Cumulative malaria parasite exposure in endemic regions often results the acquisition of partial immunity and asymptomatic infections. There is limited information on how host-parasite interactions mediate maintenance chronic symptomless infections that sustain transmission. Methods Here, we determined gene expression profiles population corresponding host peripheral blood mononuclear cells (PBMCs) from 21 children (< 15 years). We compared who were defined as...
<ns4:p><ns4:bold>Background.</ns4:bold> International recommendations for the control of coronavirus disease 2019 (COVID-19) pandemic emphasize central role laboratory testing severe acute respiratory syndrome 2 (SARS-CoV-2), etiological agent, at scale. The availability reagents, equipment and qualified staff are important bottlenecks to achieving this. Elsewhere, pooled (i.e. combining multiple samples in same reaction) has been suggested increase capacities period.</ns4:p><ns4:p>...
Background Plasmodium falciparum variant surface antigens (VSAs) contribute to malaria pathogenesis by mediating cytoadhesion of infected red blood cells the microvasculature endothelium. In this study, we investigated association between anti-VSA antibodies and clinical outcome in a controlled human infection (CHMI) study. Method We used flow cytometry ELISA measure levels IgG VSAs five heterologous one homologous P. parasite isolates, two PfEMP1 DBLβ domains samples collected day before...
Background. International recommendations for the control of coronavirus disease 2019 (COVID-19) pandemic emphasize central role laboratory testing severe acute respiratory syndrome 2 (SARS-CoV-2), etiological agent, at scale. The availability reagents, equipment and qualified staff are important bottlenecks to achieving this. Elsewhere, pooled (i.e. combining multiple samples in same reaction) has been suggested increase capacities period. Methods. We discuss our experience with SARS-CoV-2...
<ns4:p><ns4:bold>Background:</ns4:bold> The global COVID-19 outbreak relies on a quantitative real-time polymerase chain reaction (qRT-PCR) for the detection of severe acute respiratory syndrome coronavirus (SARS-CoV-2), to facilitate roll-out patient care and infection control measures. There are several qRT-PCR assays with little evidence their comparability. We report alterations developers’ recommendations sustain testing capability in our setting, where supply reagents is...
The impact of cerebral malaria on the transcriptional profiles tissues is difficult to study using noninvasive approaches. We isolated plasma extracellular vesicles (EVs) from patients with and community controls sequenced their mRNA content. Deconvolution analysis revealed that EVs are enriched in transcripts brain origin. ordered based EV-transcriptional cross-sectionally collected samples inferred disease trajectory while healthy as a starting point. found neuronal decreased trajectory,...
<ns4:p><ns4:bold>Background:</ns4:bold> The COVID-19 pandemic relies on real-time polymerase chain reaction (qRT-PCR) for the detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), to facilitate roll-out patient care and infection control measures. There are several qRT-PCR assays with little evidence their comparability. We report alterations developers’ recommendations sustain testing capability in a resource-limited setting.</ns4:p><ns4:p> <ns4:bold>Methods:</ns4:bold>...
Abstract Although most children with cerebral malaria fully recover, about a fifth of the survivors develop post-discharge neurodevelopmental sequelae suggestive advanced neuronal injury. However, molecular processes initiating neurological dysfunction in are still debatable. In this article, we explore available data and hypothesise that might be linked to APOE-mediated amyloidosis, primary pathological process initiates neurodegeneration Alzheimer's disease. If our hypothesis is tested...
<ns7:p><ns7:bold>Background: </ns7:bold>Exagerated immune activation has a key role in the pathogenesis of malaria<ns7:bold>. </ns7:bold>During blood-stage infection, <ns7:italic>Plasmodium falciparum</ns7:italic> can interact directly with host cells through infected red blood (<ns7:italic>Pf</ns7:italic>iRBCs), or indirectly by release extracellular vesicles (EVs). Here, we compared impact <ns7:italic>Pf</ns7:italic>iRBCs and <ns7:italic>P. small-sized EVs (<ns7:italic>Pf</ns7:italic>sEVs,...
<ns3:p><ns3:bold>Background: </ns3:bold>Exagerated immune activation has a key role in the pathogenesis of malaria<ns3:bold>. </ns3:bold>During blood-stage infection, <ns3:italic>Plasmodium falciparum</ns3:italic> can interact directly with host cells through infected red blood (<ns3:italic>Pf</ns3:italic>iRBCs), or indirectly by release extracellular vesicles (EVs). Here, we compared impact <ns3:italic>Pf</ns3:italic>iRBCs and <ns3:italic>P. small-sized EVs (<ns3:italic>Pf</ns3:italic>sEVs,...
Abstract Cumulative malaria parasite exposure in endemic regions often results the acquisition of partial immunity and asymptomatic infections. There is limited information on how host-parasite interactions mediate maintenance chronic symptomless infections that sustain transmission. Here, we have determined gene expression profiles population corresponding host peripheral blood mononuclear cells (PBMCs) from 21 children (< 15 years). We compared who were defined as uninfected, those with...
Abstract The impact of cerebral malaria on the transcriptional profiles tissue is difficult to study using non-invasive approaches. We isolated plasma extracellular vesicles (EVs) from patients with and community controls sequenced their RNA content. Deconvolution origins EV-RNA revealed that EVs are predominantly enriched in transcripts brain origin. Next, we used manifold learning EV-RNAseq data determine pseudotime against control samples as baseline reference. found neuronal decreased...