The Pseudomonas aeruginosa capsule, composed of polysaccharide alginate, is an important virulence factor encountered primarily in cystic fibrosis. regulatory algR gene positively controls transcription a key alginate biosynthetic gene, algD. was subcloned and sequenced by creating set nested deletions M13 bacteriophage. DNA sequence analysis revealed the homology its product with recently recognized class environmentally responsive bacterial genes, including ompR, phoB, sfrA, ntrC, spoOA,...

Amylase is an important enzyme having a varied range of industrial applications from food to cosmetics, pharmaceutical detergent industry, etc. The present study was carried out considering these amylase enzyme. Monascus sanguineus also has not been explored for its efficiency produce enzymes under solid state fermentation. In the study, various substrates were screened and among them beetroot as substrate given maximum yield (0.029 U/mL). Enzyme activity further optimized by response...

The aim of this study was to screen and optimize γ-aminobutyric acid (GABA) production from Monascus sanguineus. Different agro-waste residues were screened for GABA production. yield confirmed by thin-layer chromatography mass spectrometry. quantified using the simple ninhydrin protocol. Plackett–Burman response surface methodology (RSM) as statistical tools applied screening optimization yield. accuracy RSM model demonstrated generating a non-statistical artificial neural network...

Lovastatin is a well-known natural statin, which used for lowering plasma cholesterol levels by inhibiting 3-hydroxy-3-methyl glutaryl coenzyme A reductase. Different strains of Aspergillus and Monascus sp. have been exploited statin production but sanguineus still unexplored. In this study, lovastatin from under solid state fermentation was optimized using response surface methodology. The value the yield 20.04 mg/gds with soybean concentration 20 g/L, CaCl2 2.5 acetic acid 25 μL inoculum...

Mineral precipitation via microbial activity is a well-known process with applications in various fields. This relevance of microbially induced calcite (MICP) has pushed researchers to explore naturally occurring MICP capable bacterial strains. The present study was performed the efficiency locally isolated strains and role guar gum, which polymer, on process. were from local soil screened for urease Further, positive strain subjected urea calcium chloride based medium investigate efficacy...

We demonstrate that Microbial Induced Calcite Precipitation (MICP) can be utilized for creation of consolidates Martian Simulant Soil (MSS) and Lunar (LSS) in the form a 'brick'. A urease producer bacterium, Sporosarcina pasteurii, was used to induce MICP process both simulant soils. An admixture guar gum as an organic polymer NiCl2, bio- catalyst enhance activity, introduced increase compressive strength biologically grown bricks. casting method slurry consisting appropriate soil microbe;...

Beneficial interactions of arbuscular mycorrhizal fungi (AM Fungi) and plant growth promoting rhizobacteria (PGPR) have an important role in keeping agriculture sustainable. The present study reports the positive effects AM (Rhizophagus intraradices, Rhizophagus fasciculatum), Burkholderia seminalis dual inoculation these two strains on Lycopersicon esculatum Capsicum annuum under drought stress conditions. Each treatment was replicated six times arranged a complete randomized block design....

Present study was carried out to develop a potent mutant for enhancing secondary metabolite production from Monascus sanguineus. Ultraviolet (UV) treatment as physical and Ethyl methane sulphonate (EMS) chemical mutagen used cultivate the mutants. All obtained mutants were screened growth pigment yield on three different synthetic media namely; potato dextrose agar (PDA), malt glucose peptone (MGPA) Czapek Dox yeast extract Agar (CDYA). MGPA found suitable whereas appreciable observed with...

Natural pigments are produced by the Monascus sp., which used for coloring food substances. The intent of this study was to optimize pigment yield and biomass from unexplored sanguineus in submerged culture under stress conditions. For inducing thermal stress, spores were incubated at various temperatures higher ranges. osmotic varied concentrations NaCl, glycerol, peptone used. medium components optimized response surface methodology (RSM). combined effects four constituents mentioned...

The aim of the present work was to study production and characterization β-glucosidase from Monascus sanguineus. Agro-waste residues were screened obtain maximum yield enzyme. Jack fruit seed best substrate for enzyme production. Studies on optimization pH temperature showed acidic favorable enzymatic activity, whereas optimum 60°C. Enzyme kinetics studies with different concentration pNPG calculated value Km approximately 0.89 mM non-linear regression 0.98 linear techniques. predominantly...

The present work explores the adaptive behavior of well-known ureolytic bacterial strain namely Sporosarcina pasteurii for microbial-induced calcite precipitation (MICP) under saline environment. MICP activity was observed up to 10% NaCl supplementation and confirmed by several characterization techniques viz. scanning electron microscopy (SEM), X-ray diffraction (XRD), thermogravimetric analysis (TGA). urease did not show appreciable decline till 5% but beyond that drastic reduction though...

Abstract We present results of our investigation microbial induced calcite precipitation for manufacturing ‘space bricks’ and a proposal study this activity in low-earth orbit (LEO). Sporosarcina pasteurii , urease producing bacterial strain was used to consolidate lunar simulant soil (LSS) the form ‘brick’ with non-trivial strength properties. Potential naturally occurring polymer namely, guar gum, as an additive investigated enhancement compressive bio-consolidated samples. Experimental...

Monascus spp. is known to produce many secondary metabolites including pigments, statin, and undesired mycotoxin. M. purpureus MTCC 410 strain was grown statically for ten days in potato dextrose broth supplemented with 1% UV sterilized amino acids. Whatmann filter No. 1 used separate the developed mycelia from get intracellular pigment as extracellular one left filtrate. The concentration estimated by calorimetric method different wavelengths expressed colour value units (CVU). presence of...