A5019742328- Genetics, Aging, and Longevity in Model Organisms
- Mitochondrial Function and Pathology
- Muscle Physiology and Disorders
- Advanced Proteomics Techniques and Applications
- Autophagy in Disease and Therapy
- Microbial Metabolic Engineering and Bioproduction
- GABA and Rice Research
- Advanced Biosensing Techniques and Applications
- Ubiquitin and proteasome pathways
- Metabolism and Genetic Disorders
- Peptidase Inhibition and Analysis
- Machine Learning in Bioinformatics
- Plant Molecular Biology Research
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Photosynthetic Processes and Mechanisms
- Biosensors and Analytical Detection
- Receptor Mechanisms and Signaling
Goethe University Frankfurt
2022-2025
KU Leuven
2018-2023
University Hospital Frankfurt
2023
Vlaams Instituut voor Biotechnologie
2020
Shenzhen Institutes of Advanced Technology
2020
Introduction: Neuropeptides are neuro-endocrine signaling molecules capable of as neurotransmitters, neuromodulators or neurohormones. Studying how neuropeptide is integrated in endocrine pathways and neuropeptides regulate endogenous processes crucial to understanding multicellular organisms respond environmental internal cues.Areas covered: This review will cover proteomics peptidomics approaches used researching peptide systems breakthroughs that were achieved this field. Both...
Transcriptome and ribosome sequencing have revealed the existence of many non-canonical transcripts, mainly containing splice variants, ncRNA, sORFs altORFs. However, identification characterization products that may be translated out these remains a challenge. Addressing this, we here report on 552 proteins variants in model organism C. elegans using tandem mass spectrometry. Aided by sequencing-based prediction, generated custom proteome database tailored to search for translation elegans....
Sample loss and contamination are critical preanalytical pitfalls in microscale proteomic applications of nonadhering cells. Common assays workflows not easily adoptable to sample sizes suspension cells due inadvertent loss. This impedes experimental manipulation limited cell samples for proteomics applications, such as encountered primary human materials. Here, we describe test a simple manual batch technique single-step 100-fold concentration scarce numbers diluted (down 5000 cells) by...
Overlapping genes are widely prevalent; however, their expression and consequences poorly understood. Here, we describe functionally characterize a novel zyx-1 overlapping gene, azyx-1 , with distinct regulatory functions in Caenorhabditis elegans . We observed conservation of alternative open reading frames (ORFs) the 5′ region zyxin family members several animal species, find shared sites proteoform C In line standard ribosome scanning model, our results support cis regulation long...
Abstract BackgroundRNA binding proteins regulate gene expression at the post-transcriptional level by controlling fate of RNA, in processes such as mRNA localization, translation, splicing and stability. The annotation RNA is mainly based on well-known domains motifs. However, novel without conventional have been identified different species using vivo interactome capture. To find support for conserved plants, we applied an optimized capture to monocot model Brachypodium distachyon....
Abstract Overlapping genes are widely prevalent, however, their expression and consequences poorly understood. Here, we describe functionally characterize a novel zyx-1 overlapping gene, azyx-1 , with distinct regulatory functions in C. elegans . We observed conservation of alternative open reading frames the 5’ region zyxin family members several animal species, find shared sites proteoform In line standard ribosome scanning model, our results support cis regulation long isoform(s) by...