Ex situ preservation is an important method in the of chickens, and cryopreservation semen only for gamete at present. During last two decades, many studies have been performed to develop standard chicken technology achieve great progress. Many attempts methods were investigated adapt subspecies or different breeds. In this paper, we firstly reviewed main factors affecting sperm, including unique structure characteristics spermatozoa. Secondly, on key points sperm technology, dilution,...

Heat stress is one of the most detrimental confrontations in tropical and subtropical regions world, causing considerable economic losses poultry production. Propolis, a resinous product worker honeybees, possesses several biological activities that could be used to alleviate deleterious effects high environmental temperature on The current study was aimed at evaluating propolis supplementation Japanese quail (Coturnix coturnix japonica) diets production performance, intestinal...

The massive meat production of broiler chickens make them continuously exposed to potential stressors that stimulate releasing stress-related hormones like corticosterone (CORT) which is responsible for specific pathways in biological mechanisms and physiological activities. Therefore, this research was conducted evaluate a wide range responses related performance, immune function, plasma biochemistry, gene expressions cell death morphology during after 7-day course CORT injection. A total...

Embryo cryopreservation remains an important technique to enhance the reconstitution and distribution of animal populations with high genetic merit. One major detrimental factors this is damage caused by oxidative stress. Melatonin widely known as antioxidant multi-faceted ways counteract In paper, we investigated role melatonin in protecting rabbit embryos during preimplantation development from potential harmful effects stress induced vitro culture or vitrification. Rabbit at morula stages...

Reactive oxygen species (ROS) and free radicals are one of the major detrimental factors that can negatively affect quality sperm during cryopreservation. Melatonin is an effective antioxidant radical scavenger in various cells. In this study, therefore, aim was to evaluate post-thawed spermatozoa after cryopreservation rooster semen freezing extender supplemented with melatonin. Semen samples from seven Green-legged Partridge roosters were pooled diluted EK 10-3, 10-6, or 10-9 M melatonin...

This study examines the effect of dietary supplementation with Lactobacillus acidophilus (LA) on cholesterol levels, immune response, and productive performance laying hens. A total 216, 40-week-old, commercial Hy-Line brown chicken layers were randomly assigned into four treatment groups (18 birds × three replicates per group) fed diet supplemented 0 (control), 1 109, 21 31 109 colony forming units (CFUs) kg feed for six consecutive weeks. Results show that plasma triglycerides, low-density...

Heat stress (HS) induces deleterious effects on the performance of laying hens and causes economic losses for poultry industry. This study was carried out to investigate organic effect selenium-enriched yeast (SY) relieving performance, immunity physiological deterioration induced by heat in hens. A total 324, 28-week-old, Hy-Line Brown commercial chicken layers were randomly distributed into 4 treatments according a 2 × factorial design, with 9 replicates per treatment (n = 81). From 30 34...

The present work was carried out to investigate the effects of dietary propolis supplementation laying Japanese quail (Coturnix coturnix japonica) on egg production, quality, physiological and immunological aspects under heat stress conditions. A total 200, 21-day-old, females were distributed equally into standard wired cages in two identical environmentally-controlled rooms (10 per room, 10 birds cage). From 29–70 d age, first room remained at a normal temperature 24°C (C group), whereas...

Sperm cryopreservation is of great importance for the poultry industry but still needs to be optimized. The high susceptibility sperm cryodamage leads low fertility rates after cryopreservation. Therefore, present study aimed at evaluating effect including a cryoprotectant, dimethylacetamide (DMA), in chicken semen freezing extenders final concentration 3%, 6%, or 9% on post-thawed motility, quality, antioxidant biomarkers, anti-freeze gene expression, and fertilizing ability. Results showed...

Paraquat (PQ) is used as a herbicide in agriculture and causes oxidative inflammatory damage to animal tissues. The current study was conducted investigate the positive effects of dietary propolis (PR), potent naturally produced antioxidant, on growth performance immune function turkey poults exposed stress induced by PQ injection. Native male (n = 120, 49-d-old) were randomly assigned into 4 groups: received basal diet with daily subcutaneous injection 5 mg/kg BW for 7 consecutive days (PQ...

Modern methods of industrial poultry and egg production systems involve stressful practices that stimulate Escherichia coli (E. coli) activity causing endotoxic shock. This investigation was conducted to evaluate the expression pro-inflammatory cytokines cell death program genes DNA damage induced by E. in brain liver tissues laying hens. A total two hundred ten H&N brown layer hens with 20 week age, were used this research. First, preliminary experiments designed (60 total) establish...

There is an extensive search for natural products that can be introduced to broiler rations improve performance, especially during the unfavorable breeding conditions. Under heat-stress conditions, immune response seriously deteriorates, which consequently impairs production performance. Thus, present study aimed investigate potentials of Citrullus colocynthis seeds (CCs) supplementation modulate broilers subjected chronic heat stress. A total 300 Cobb-500 male chickens aged 21 days were...

The long-term semen cryopreservation is increasingly crucial for conservation of endangered livestock and poultry species. Glycerol the most widely used cryoprotectant freezing chicken semen. Continuous improvement in details with glycerol may help increase fertility post-thawed Two experiments were performed present study to investigate effects concentration, removal method, straw type on quality sperm. In experiment 1, concentration (3%, 5%, 7%, 9%, 11%, 13%) method (final dilution ratio...

Cryopreservation of rooster semen is essential for conserving genetic resources, improvement, and increasing productivity. However, the nature avian sperm presents a global issue in ensuring superior frozen artificial insemination. Thus, present study aimed to evaluate impact using dimethylacetamide (DMA), dimethyl sulfoxide (DMSO), ethylene glycol (EG) as cryoprotectants on post-thawed motility, quality, antioxidant indicators, fertilizing capacity. Twice week, fresh ejaculates were...

This study aimed to investigate the role of dietary Spirulina platensis (SP) supplementation in relieving negative impacts heat stress (HS) on productive performance, cholesterol profile, redox status, and inflammatory cytokines laying hens. A total 288, 45-wk-old 1550.7 ± 2.3 g initial body weight, HY-Line W-36 hens were housed two environmental-controlled compartments. Layers allotted eight treatments a × four factorial design, with six replicates containing birds per treatment. The...

Spirulina platensisis (SP) is a blue-green microalgae with high value for animal and poultry nutrition. The study employed 250 40-week-old, HY-Line W-36 commercial laying hens. layers received one of five experimental diet substitutes in groups 10 consecutive weeks (five replicates hens each group); soybean-corn basal formulation without SP (Control group) or the soybean partially substituted 3% SP, 6% 9% 12% (for remaining four groups). results showed that dietary treatment significantly (p...

Citrullus colocynthis (CC) has been known as a natural medicinal plant with wide biological activities, including antioxidant, anti-inflammatory, and antilipidemic effects. The aim of this study was to investigate the effect inclusion ethanolic extract CC seeds (ECCs) into layer diets on lipid profile, stress indicators, physiological productive performance laying hens. A total 216 forty-week-old commercial Hy-Line brown hens were randomly assigned four equal groups (3 birds × 18 replicates...

This study was conducted to elucidate the effect of different freezing extenders on two lines selected for hyperprolificacy and longevity (H LP, respectively). In extender A, dimethyl sulphoxide (Me2SO) sucrose were used as cryoprotectants. B C, replaced by 20% egg yolk, in C Me2SO substituted acetamide. Semen packaged 0.25 ml plastic straws cooled at 5°C 45 min, then frozen liquid nitrogen vapour 10 min before being plunged into nitrogen. Thawing carried out immersing a water bath 50°C s....

Summary Oxidative stress is a major cause of defective embryo development during in vitro culture. Retinoids are recognized as non-enzymatic antioxidants and may have an important role the regulation cell differentiation vertebrate development. However, there not enough reports discussing antioxidant developmental capacity retinoids, including retinol (RT), on embryos recovered from livestock animals, particularly rabbit species. Therefore, morula obtained nulliparous Red Baladi does were...

Sperm cryopreservation is an effective technique for conserving animal genetic diversity and transmitting superior backgrounds, maintained via a non-invasive sampling collection of huge quantities sperm. Nevertheless, in avian species not commercially viable because the rooster sperm's susceptibility to damage. This study aims estimate impact dimethylacetamide (DMA) as cryoprotectant at different levels (3%, 6%, or 9%) on post-thawed sperm quality, motility, antioxidant-biomarkers,...

Summary This study aimed to investigate the effect of melatonin supplementation at different levels in culture medium on embryo development rabbits. Embryos 2–4 cells, 8–16 cells and morula stages were recovered from nulliparous Red Baladi rabbit does by laparotomy technique 24, 48 72 h post-insemination, respectively. Normal embryos each stage cultured hatched blastocyst either control (TCM-199 + 20% fetal bovine serum) or supplemented with 10 −3 M, −6 M −9 M. No was found 24...