A5070499202- Advanced biosensing and bioanalysis techniques
- Microtubule and mitosis dynamics
- DNA and Nucleic Acid Chemistry
- bioluminescence and chemiluminescence research
- Click Chemistry and Applications
- Photochromic and Fluorescence Chemistry
- Molecular Junctions and Nanostructures
- Micro and Nano Robotics
- Radical Photochemical Reactions
- Cellular transport and secretion
- Chemokine receptors and signaling
- Cell Adhesion Molecules Research
- Chemical Synthesis and Analysis
- Bacteriophages and microbial interactions
- Photodynamic Therapy Research Studies
- CO2 Reduction Techniques and Catalysts
- Protist diversity and phylogeny
- Photosynthetic Processes and Mechanisms
- Atrial Fibrillation Management and Outcomes
- HER2/EGFR in Cancer Research
- Advanced Materials and Mechanics
- Quantum-Dot Cellular Automata
- Catalytic C–H Functionalization Methods
- Cancer Research and Treatments
- Cancer Cells and Metastasis
University of Geneva
2018-2024
NCCR Chemical Biology - Visualisation and Control of Biological Processes Using Chemistry
2018-2024
University of Milan
2017
Abstract Drugs are administered at a dosing schedule set by their therapeutic index, and termination of action is achieved clearance metabolism the drug. In some cases, such as anticoagulant drugs or immunotherapeutics, it important to be able quickly reverse drug’s action. Here, we report general strategy achieve on-demand reversibility designing supramolecular drug (a noncovalent assembly two cooperatively interacting fragments held together transient hybridization peptide nucleic acid...
DNA-based circuitry empowers logic gated operations and amplifications but is restricted to nucleic acid output. Templated reactions enable the translation of cues into diverse small-molecule outputs are more limited in their amplification. Herein, we demonstrate coupling a DNA circuit templated order achieve high levels amplification output small molecules, response input. We that allows for detection fM concentration analyte can respond with release cytotoxic drug.
The HCR represents a powerful tool for amplification in DNA-based circuitry and sensing applications, yet requires the use of long DNA sequences to grant hairpin metastability. Here we describe minimal system based on peptide nucleic acids (PNAs). A comprising 5-mer stem loop/toehold hairpins was found be suitable achieve rapid amplification. These were shown yield >10-fold 2 h detection cancer biomarker live cells. γ-peg-modified PNA beneficial.
Bioluminescence resonance energy transfer (BRET) is extensively used to study dynamic systems and has been utilized in sensors for studying protein proximity, metabolites, drug concentrations. Herein, we demonstrate that BRET can activate a ruthenium-based photocatalyst which performs bioorthogonal reactions. from luciferase the ruthenium uncage effector molecules with up 64 turnovers of catalyst, achieving concentrations >0.6 μM 10 nM construct. Using sensor, further catalysis be modulated...
Kinesin-1 is a processive motor protein that uses ATP-derived energy to transport variety of intracellular cargoes toward the cell periphery. The ability visualize and monitor kinesin in live cells critical study myriad functions associated with cargo trafficking. Herein we report discovery fluorogenic small molecule substrate (QPD-OTf) for kinesin-1 yields precipitating dye along its walking path on microtubules (MTs). QPD-OTf enables native activity cellulo without external modifications....
Abstract Bioluminescence resonance energy transfer (BRET) has been widely used for studying dynamic processes in biological systems such as protein–protein interactions and other signaling events. Aside from acting a reporter, BRET can also turn on functions living systems. Herein, we report the application of to performing biorthogonal reaction cells; namely, releasing functional molecules through coumarin molecule, process termed bioluminolysis. An efficient Nanoluc‐Halotag chimera protein...
Herein we report the first example of an isoDGR-drug conjugate (2), designed to release paclitaxel selectively within cancer cells expressing integrin α
Abstract Drugs are administered at a dosing schedule set by their therapeutic index and termination of action is achieved clearance metabolism the drug (hours to days for small molecules, weeks months biologics). In some cases, it important achieve fast reversal drug’s overcome dangerous side effects or in response unforeseen events. A case point anticoagulant drugs. Here we report general strategy on-demand reversibility leveraging supramolecular assembly fragments showcase approach with...
Abstract Bioluminescence resonance energy transfer (BRET) has been widely used for studying dynamic processes in biological systems such as protein–protein interactions and other signaling events. Aside from acting a reporter, BRET can also turn on functions living systems. Herein, we report the application of to performing biorthogonal reaction cells; namely, releasing functional molecules through coumarin molecule, process termed bioluminolysis. An efficient Nanoluc‐Halotag chimera protein...
Dimeric proteins are prominent in biology, and receptor dimerization (homo- or heterodimerization) is central to signal transduction. Herein, we report a network that responds membrane-associated dimeric protein with the uncaging of powerful cytotoxic. The based on two ligands functionalized peptide nucleic acids (PNAs) (templating strand catalyst-functionalized strand, respectively) substrate caged cytotoxic (monomethyl auristatin E: MMAE; high-affinity tubulin ligand). In presence protein,...
Abstract Kinesin-1 is a processive motor protein that uses ATP-derived energy to transport variety of intracellular cargoes toward the cell periphery. As tracks for cargo delivery, kinesin-1 subset microtubules within dense microtubule network. It still debated what defines specific binding microtubules. Therefore, ability visualize and monitor kinesin in live cells critical study myriad functions associated with trafficking. Herein we report discovery fluorogenic small molecule substrate...