A5069722751- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Surface Modification and Superhydrophobicity
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Photosynthetic Processes and Mechanisms
- Redox biology and oxidative stress
- Advanced Sensor and Energy Harvesting Materials
- Polymer crystallization and properties
- Amino Acid Enzymes and Metabolism
- Material Properties and Processing
- Protein Structure and Dynamics
- Photoreceptor and optogenetics research
- Asphalt Pavement Performance Evaluation
- Enzyme Production and Characterization
- Infrastructure Maintenance and Monitoring
- Virology and Viral Diseases
- Cancer-related Molecular Pathways
- Polysaccharides and Plant Cell Walls
- Injury Epidemiology and Prevention
- Polymer Nanocomposite Synthesis and Irradiation
- Organic Light-Emitting Diodes Research
- Virus-based gene therapy research
- Microbial Metabolites in Food Biotechnology
- Transportation Safety and Impact Analysis
RISE Research Institutes of Sweden
2019-2024
Åbo Akademi University
2010
Uppsala University
2000-2009
Centre National de la Recherche Scientifique
1999-2004
Architecture et Fonction des Macromolécules Biologiques
2003-2004
Swedish University of Agricultural Sciences
1998-2003
Roskilde University
2003
Technical University of Denmark
2003
McGill University
2002
Kyoto Bunkyo University
2002
The nucleoprotein of measles virus consists an N-terminal moiety, N(CORE), resistant to proteolysis and a C-terminal N(TAIL), hypersensitive not visible as distinct domain by electron microscopy. We report the bacterial expression, purification, characterization N(TAIL). Using nuclear magnetic resonance, circular dichroism, gel filtration, dynamic light scattering, small angle x-ray we show that N(TAIL) is structured in solution. Its sequence spectroscopic hydrodynamic properties indicate...
Measles virus is a negative-sense, single-stranded RNA belonging to the Mononegavirales order which comprises several human pathogens such as Ebola, Nipah, and Hendra viruses. The phosphoprotein of measles modular protein consisting an intrinsically disordered N-terminal domain (Karlin, D., Longhi, S., Receveur, V., Canard, B. (2002) Virology 296, 251-262) C-terminal moiety (PCT) composed alternating globular regions. We report crystal structure extreme (XD) (aa 459-507) at 1.8 A resolution....
Pectin is a principal component in the primary cell wall of plants. During development, pectin modified by methylesterases to give different properties wall. This report describes first crystal structure plant methylesterase. The β‐helical embodies central cleft, lined several aromatic residues, that has been deduced be suitable for binding. active site found at center this cleft where Asp157 suggested act as nucleophile, Asp136 an acid/base and Gln113/Gln135 form anion hole stabilize...
Abstract The three‐dimensional structure of betaine aldehyde dehydrogenase, the most abundant dehydrogenase (ALDH) cod liver, has been determined at 2.1 Å resolution by X‐ray crysallographic method molecular replacement. This enzyme represents a novel highly multiple ALDH, with least 12 distinct classes in humans. ALDH class 9 is different from two recently structures (classes 2 and 3). Like these, three domains, one coenzyme binding domain, catalytic oligomerization domain. Crystals grown...
Objective– To evaluate the effects of continuous duodenal infusion levodopa over time on disabling fluctuations in motor performance advanced parkinsonian patients. It has earlier been demonstrated that these can be reduced by keeping plasma concentration constant. Material and methods– In view low water solubility a stable dispersion drug was developed used for intraduodenal patients with Parkinson's disease. Nine were evaluated respect to an optimal oral treatment, during nasoduodenal...
Some key chloroplast enzymes are activated by light via a ferredoxin−thioredoxin reduction system which reduces disulfide bridges in the enzymes. We describe for first time structural basis redox activation of enzyme, NADP-dependent malate dehydrogenase (MDH) from Sorghum vulgare whose structure has been determined and refined at 2.4 Å resolution. In addition to normal components MDHs, enzyme exhibits extensions both N- C-termini, each contains regulatory bridge must be reduced activation....
Abstract Thin plasma polymerized layers of acrylic acid (PPAA) were deposited onto polyethylene and muscovite mica surfaces. Structure surface properties the layer depend on polymerization conditions. The content carboxylic groups in decreases, whereas degree crosslinking or branching increases, with increasing discharge power. A soft, sticky a low contact angle against water is obtained when power (5 W) used. In contrast, hard film rather high (50 W). force apparatus was employed to study...
Deoxyribonucleoside kinases are feedback inhibited by the final products of salvage pathway, deoxyribonucleoside triphosphates. In present study, mechanism inhibition is presented based on crystal structure a complex between fruit fly kinase and its inhibitor deoxythymidine triphosphate. The was found to be bound as bisubstrate with nucleoside part in binding site phosphate groups partially occupying donor site. overall enzyme−inhibitor very similar enzyme−substrate complexes deoxycytidine,...
Abstract The Adhesion between plasma‐modified polyethylene (PE) and different polar polymers, as well aluminium steel, has been studied. PE was modified by either oxygen plasma treatment or polymerization of acrylic acid. result show the importance introducing groups on surface non‐polar in order to improve adhesion polymers metals. Laminates made ad polyamide 6 (PA‐6) poly(ethylene vinyl alcohol) (EVAL‐G) showed failure PA‐6 EVAL‐G when delaminated. Poly(ethylene terephthalate) (PET), which...
The chloroplastic nicotinamide adenine dinucleotide phosphate‐malate dehydrogenase (NADP‐MDH) (EC 1.1.1.82), a key enzyme of photosynthetic carbon assimilation the C4 NADP‐malic type plants, is strictly regulated by light through ferredoxin‐thioredoxin system. It inactive in dark, oxidized form, and activated reduction specific regulatory disulfides. A site‐directed mutagenesis approach allowed localization disulfides N‐ C‐terminal sequence extensions conserved all light‐regulated malate...
The substitution of mineral aggregates with crumb rubber (CR) from waste end-of-life tires (ELTs) in the asphalt concretes, has been considered a sustainable paving industry approach. used to construct pavements proven enhanced resilience and improved durability. However, some issues related rubber’s surface adhesion or swelling may arise these practices generate complications (binder consumption, temperatures, mixing times). One possible solution overcome materials’ compatibility problems...
Cyclists, pedestrians and elderly people’s specific needs in urban road infrastructures are often neglected. They rarely benefit from safety measures or innovations. Inspired by playgrounds aiming to reduce vulnerable users (VRUs) injuries, the development of rubber-based impact-absorbing pavements (IAP) offers a possibility rethink design address on roads, which constitutes major challenge terms attaining more sustainable, resilient, safe cities. Therefore, bituminous mixtures with four...
The chloroplastic NADP malate dehydrogenase is completely inactive in its oxidized form and activated by thiol/disulfide interchange with reduced thioredoxin. To elucidate the molecular mechanism underlying absence of activity enzyme, we used site-directed mutagenesis to delete or substitute two most C-terminal residues (C-terminal Val, penultimate Glu, both bearing negative charges). We also combined these mutations elimination one possible regulatory N-terminal disulfides mutating...