A5102716302- Intraperitoneal and Appendiceal Malignancies
- Connexins and lens biology
- Fibroblast Growth Factor Research
- Corneal Surgery and Treatments
- Occupational and environmental lung diseases
- Urological Disorders and Treatments
- Ovarian cancer diagnosis and treatment
- interferon and immune responses
- Connective tissue disorders research
- Myasthenia Gravis and Thymoma
- Developmental Biology and Gene Regulation
- Pancreatic and Hepatic Oncology Research
- RNA Interference and Gene Delivery
- Colorectal Cancer Treatments and Studies
- Immune cells in cancer
- Cellular transport and secretion
- Echinoderm biology and ecology
- Hedgehog Signaling Pathway Studies
- Neonatal Respiratory Health Research
- Heat shock proteins research
- Hepatitis B Virus Studies
- FOXO transcription factor regulation
- Intraocular Surgery and Lenses
- Wnt/β-catenin signaling in development and cancer
- Appendicitis Diagnosis and Management
University of Miami
2024-2025
Sylvester Comprehensive Cancer Center
2025
Creighton University
2009-2023
CHI Health Creighton University Medical Center - Bergan Mercy
2009-2015
Baylor College of Medicine
2000-2013
University of Delaware
2013
Albert Einstein College of Medicine
2013
University of Oklahoma Health Sciences Center
2009
University of Houston
1995-1997
ABSTRACT We investigated the mechanism of tissue induction and specification using lacrimal gland as a model system. This structure begins its morphogenesis bud-like outgrowth conjunctival epithelium ultimately forms branched with secretory function. Using reporter transgene specific marker for epithelium, we show that transcription factor Pax6 is required normal development probably an important competence factor. In investigating cell-cell signaling required, fibroblast growth (FGF) 10...
The nomenclature and classification of pseudomyxoma peritonei (PMP) is confusing controversial. Numerous systems have been proposed, none which are easily reproducible or a useful guide for treatment. Patients with PMP appendiceal origin were identified from our institution's database. Kaplan-Meier analyses performed based on proposed new classification, three-tiered grading system designated PMP1, PMP2, PMP3. These results compared the established schemes by Ronnett Bradley et al. There 211...
Abstract Lens induction is a classical embryologic model to study cell fate determination. It has been proposed earlier that specific changes in core histone modifications accompany the process of specification and The lysine acetyltransferases CBP p300 function as principal enzymes modify histones facilitate gene expression. Herein, we performed conditional inactivation both ectodermal cells give rise lens placode. Inactivation resulted dramatic discontinuation all aspects organogenesis,...
ABSTRACT The vertebrate lens has a distinct polarity with cuboidal epithelial cells on the anterior side and differentiated fiber posterior side. It been proposed that anterior-posterior of is imposed by factors present in ocular media surrounding (aqueous vitreous humor). differentiation have hypothesized to be members fibroblast growth factor (FGF) family. Though FGFs shown sufficient for induction both vivo vitro, they not demonstrated necessary endogenous initiation cell differentiation....
Abstract Background The C-terminal Eps15 homology domain-containing protein 1 (EHD1) is ubiquitously expressed and regulates the endocytic trafficking recycling of membrane components several transmembrane receptors. To elucidate function EHD1 in mammalian development, we generated Ehd1 -/- mice using a Cre/ loxP system. Results Both male female survived at sub-Mendelian ratios. A proportion were viable showed smaller size birth, which continued into adulthood. adult males infertile...
INTRODUCTION: Even given standard of care and the advent new therapies, glioblastoma (GBM) is one deadliest forms cancer. Laser interstitial thermal therapy(LITT) a minimally invasive technique used to ablate intra-axial brain tumors. Non-ablative LITT-induced hyperthermia (33-43°C) increases intra-tumoral mutational burden neoantigen production. Hyperthermia promotes presentation via release heat shock proteins(HSPs). HSPs are protein-folding chaperones that can present novel intracellular...
Viral mimicry refers to the activation of innate antiviral immune responses due induction endogenous retroelements (REs). augments antitumor and sensitizes solid tumors immunotherapy. Here, we found that targeting what believe be a novel, master epigenetic regulator, Zinc Finger Protein 638 (ZNF638), induces viral in glioblastoma (GBM) preclinical models potentiates checkpoint inhibition (ICI). ZNF638 recruits HUSH complex, which precipitates repressive H3K9me3 marks on REs. In GBM, is...
Abstract Background The flat bones of the skull (i.e., frontal and parietal bones) normally form through intramembranous ossification. At these sites cranial mesenchymal cells directly differentiate into osteoblasts without formation a cartilage intermediate. This type ossification is distinct from endochondral ossification, process that involves initial later replacement by bone. Results We have analyzed line transgenic mice expresses FGF9, member fibroblast growth factor family (FGF), in...
There is a paucity of information about the molecular perturbations involved in MPM tumor formation. We previously reported that EGFR-TK mutations were predictive achieving optimal surgical cytoreduction, but status EGFR pathway activation potential these was not known. Here we present mutant activating and matured survival data mutant(mut+) relative to wild type EGFR(mut-) mesothelioma. Twenty-nine patients evaluated their tumors probed for catalytic TK-domain. Twenty-five treated with...
Purpose.: The studies reported here were performed to analyze the roles of Sproutys (Sprys), downstream targets and negative feedback regulators fibroblast growth factor (FGF) signaling pathway, in lens corneal differentiation. Methods.: Spry1 -2 conditionally deleted epithelial precursors using Le-Cre transgene floxed alleles -2. Alterations development assessed by hematoxylin eosin staining, situ hybridization, immunohistochemistry. Results.: upregulated fibers at onset fiber FGF was both...
ABSTRACT Little is known about the cell signaling involved in forming body plan of sea urchin embryo. Previous work suggested that PDGF-like and EGF-like receptor-mediated pathways are gastrulation spicu-logenesis Lytechinus pictus Here we show expression human PDGF receptor-β lacking cytoplasmic domain disrupted development a manner consistent with dominant/negative mechanism. The truncated inhibited gut spicule formation differentiation along oral-aboral axis. most severely affected...
Abstract Background Mammalian Ras genes regulate diverse cellular processes including proliferation and differentiation are frequently mutated in human cancers. Tumor development response to activation varies between different tissues the molecular basis for these variations poorly understood. The murine lens cornea have a common embryonic origin arise from adjacent regions of surface ectoderm. Activation fibroblast growth factor (FGF) signaling pathway induces corneal epithelial cells...
Diffuse malignant peritoneal mesothelioma is a rare, aggressive disease. Cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) have improved outcomes where systemic has not succeeded. In this study, we compare of patients treated with mitomycin or carboplatin as perfusate. retrospective 47 procedures (CRS + HIPEC) were conducted on 44 between March 2003 August 2010 either carboplatin. χ 2 Student's t test used for comparison clinicopathological variables...
Abstract Pseudomyxoma peritonei ( PMP ) is a neoplastic syndrome characterized by peritoneal tumor implants with copious mucinous ascites. The standard of care for patients aggressive cytoreductive surgery performed in conjunction heated intraperitoneal chemotherapy. Not all are candidates these procedures and majority the will have recurrent disease. In addition to secreted mucin, inflammation fibrosis central pathogenesis but molecular processes that regulate tumor‐stromal interactions...
The phosphatidylinositol (PI3K)/AKT/mTOR axis represents an important therapeutic target to treat human cancers. A well-described downstream of the PI3K pathway is forkhead box O (FOXO) transcription factor family. FOXOs have been implicated in many cellular responses, including drug-induced resistance cancer cells. However, FOXO-dependent acute phase mediated by pictilisib, a potent small molecule inhibitor (PI3Ki), has not studied. Here, we report that pictilisib-induced adaptive regulated...
Mucinous colorectal adenocarcinomas (MCAs) are clinically and morphologically distinct from nonmucinous cancers (CRCs), show a spectrum of genetic alterations (higher KRAS mutations, lower p53, high MUC2), exhibit more aggressive behavior (more prone to peritoneal dissemination lymph node involvement) associated with poorer response chemotherapy limited treatment options. Here, we report the effectiveness combinatorial targeting two KRAS-mediated parallel pathways in reducing MUC2 production...
Growth factor signaling is implicated in the regulation of lens cell proliferation and differentiation during development. Activation growth receptor tyrosine kinases known to activate Ras proteins, small GTP-binding proteins that function as part signal transduction machinery. In present study, we examined which classical genes are expressed cells normal development whether expression an activated version sufficient induce either or fiber transgenic mice. situ hybridization showed H-Ras,...