A5039390726- Cancer, Hypoxia, and Metabolism
- Medical Imaging and Pathology Studies
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Sex and Gender in Healthcare
- Neuroblastoma Research and Treatments
- Chromosomal and Genetic Variations
- Advanced biosensing and bioanalysis techniques
- Protein Degradation and Inhibitors
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Chemokine receptors and signaling
- Interdisciplinary Research and Collaboration
- Neuropeptides and Animal Physiology
- Cancer, Stress, Anesthesia, and Immune Response
- Career Development and Diversity
University of California, San Francisco
2023-2025
Gladstone Institutes
2025
UCSF Helen Diller Family Comprehensive Cancer Center
2023-2024
Georgetown University
2019-2022
Georgetown University Medical Center
2019-2022
Abstract Multiplexed genetic perturbations are critical for testing functional interactions among coding or non-coding elements. Compared to double-stranded DNA cutting, repressive chromatin formation using CRISPR interference (CRISPRi) avoids genotoxicity and is more effective perturbing regulatory elements in pooled assays. However, current CRISPRi screening approaches limited targeting one three genomic sites per cell. We engineer an Acidaminococcus Cas12a (AsCas12a) variant, multiplexed...
Gender inequities persist in science, with women encountering significant barriers at various career stages, particularly fields such as Immunology. This article highlights the work of Immunologists for Equity (IgEquity), a trainee-led organization within ImmunoX Program University California, San Francisco (UCSF), which is committed to addressing these disparities. Through initiatives focused on community building, mentorship, outreach and advocacy, IgEquity seeks advance gender equity...
Neuropeptide Y (NPY) is a pleiotropic peptide, which involved in many biological mechanisms important regulation of cell growth and survival. The aim this study was comprehensive analysis the NPY system prostate pathology.The based on immunohistochemical its receptors, Y1R, Y2R Y5R, tissue samples from benign (BP), primary cancer (PCa) PCa bone metastases. Tissue microarray (TMA) technique employed, with multiple cores each specimen. Intensity immunoreactivity expression index (EI), as well...
Abstract Multiplexed genetic perturbations are critical for testing functional interactions among coding or non-coding elements. Compared to double-stranded DNA cutting, repressive chromatin formation using CRISPR interference (CRISPRi) avoids genotoxicity and is more effective perturbing regulatory elements in pooled assays. However, current CRISPRi screening approaches limited targeting 1-3 genomic sites per cell. To develop a tool higher-order ( > 3) combinatorial of with genomics...
Abstract Ewing Sarcoma (ES) is an aggressive malignancy that arises in children and young adults. Although the survival for patients with localized tumors relatively high, metastatic form carries a dismal prognosis, particularly when bone metastases are present. The frequency of osseous dissemination increases necrotic ES tumors. In line this, hypoxia, major cause tumor necrosis, has been shown to increase potential cells. Previous data from our laboratory demonstrated orthotopic xenograft...
Ewing Sarcoma (ES) is an aggressive malignancy that arises in children and young adults. Although the survival for patients with localized tumors relatively high, metastatic form carries a dismal prognosis, particularly when bone metastases are present. The frequency of osseous dissemination increases necrotic ES tumors. In line this, hypoxia, major cause tumor necrosis, has been shown to increase potential cells. Previous data from our laboratory demonstrated orthotopic xenograft model,...