A5091410082- Lipid metabolism and biosynthesis
- Hormonal Regulation and Hypertension
- Pharmacogenetics and Drug Metabolism
- Drug Transport and Resistance Mechanisms
- Autophagy in Disease and Therapy
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Sphingolipid Metabolism and Signaling
- Histone Deacetylase Inhibitors Research
- IoT and Edge/Fog Computing
- Metabolism and Genetic Disorders
- Biosimilars and Bioanalytical Methods
- Age of Information Optimization
- Sulfur Compounds in Biology
- Antibiotics Pharmacokinetics and Efficacy
- Reproductive System and Pregnancy
- Pancreatic function and diabetes
- RNA modifications and cancer
- Analytical Methods in Pharmaceuticals
- Plant responses to water stress
- Advanced Neural Network Applications
- Coffee research and impacts
- Estrogen and related hormone effects
- Ubiquitin and proteasome pathways
- Eicosanoids and Hypertension Pharmacology
National Institute of Diabetes and Digestive and Kidney Diseases
2025
University of Minnesota
2020-2023
Stowers Institute for Medical Research
2020
University of Georgia
2020
Kansas City University
2020
Chinese Academy of Sciences
2010-2019
Institute of Computing Technology
2019
University of Kansas
2012-2016
University of California, San Diego
2015
Shanghai Institute of Materia Medica
2010
The hepatotoxicity of thioacetamide (TA) has been known since 1948. In rats, single doses cause centrolobular necrosis accompanied by increases in plasma transaminases and bilirubin. To elicit these effects, TA requires oxidative bioactivation, leading first to its S-oxide (TASO) then chemically reactive S,S-dioxide (TASO2), which ultimately modifies amine-lipids proteins. generate a suite liver proteins adducted metabolites for proteomic analysis reduce the need both animals labeled...
The autophagic degradation of lipid droplets (LDs), termed lipophagy, is a major mechanism that contributes to turnover in numerous cell types. While factors, including nutrient deprivation or overexpression PNPLA2/ATGL (patatin-like phospholipase domain containing 2) drive the trafficking fatty acids (FAs) produced from this pathway largely unknown. Herein, we show PNPLA2 and promoted extracellular efflux FAs. Inhibition autophagy lysosomal attenuated FA highlighting critical role for...
Organelle interactions play a significant role in compartmentalizing metabolism and signaling. Lipid droplets (LDs) interact with numerous organelles, including mitochondria, which is largely assumed to facilitate lipid transfer catabolism. However, quantitative proteomics of hepatic peridroplet mitochondria (PDM) cytosolic (CM) reveals that CM are enriched proteins comprising various oxidative pathways, whereas PDM involved anabolism. Isotope tracing super-resolution imaging confirms fatty...
Lipid droplets (LDs) are energy-storage organelles that coated with hundreds of proteins, including members the perilipin (PLIN) family. PLIN5 is highly expressed in oxidative tissues, liver, and thought to play a key role uncoupling LD accumulation from lipotoxicity; however, mechanisms behind this action incompletely defined. We investigated hepatic inflammation lipotoxicity murine model under both fasting refeeding conditions hepatocyte cultures. ablation antisense oligonucleotides...
Bacterial sepsis is characterized by a rapid increase in the expression of inflammatory mediators to initiate acute phase response liver. Inflammatory mediator release counterbalanced coordinated anti-inflammatory molecules such as interleukin 1 receptor antagonist (IL1-Ra) through time. This study determined whether activation pregnane X (PXR, NR1I2) alters lipopolysaccharide (LPS)-inducible gene program primary cultures hepatocytes (PCHs). Preactivation PXR for 24 hours PCHs isolated from...
Mitochondrial endonuclease G (EndoG) contributes to chromosomal degradation when it is released from mitochondria during apoptosis. It presumed also have a mitochondrial function because EndoG deficiency causes dysfunction. However, the mechanism by which regulates not known. Fat accumulation in metabolic dysfunction–associated steatotic liver disease (MASLD), more common men, caused part expression reduced MASLD liver, and an obesity-independent manner but only males. promotes respiration...
Several nuclear receptor (NR) superfamily members are known to be the molecular target of either small ubiquitin-related modifier (SUMO) or ubiquitin-signaling pathways. However, little is currently regarding how these two post-translational modifications interact control NR biology. We show that SUMO and ubiquitin circuitry coordinately modifies pregnane X (PXR, NR1I2) play a key role in regulating PXR protein stability, transactivation capacity, transcriptional repression. The SUMOylation...
The surging Deep Neural Network (DNN) based applications are becoming increasingly popular in mobile computing. However, they impose significant challenges for computing, as DNN tasks lead to much more computation complexity and data volume compared with traditional tasks. To alleviate this, edge computing (MEC) provides a feasible approach through task partitioning offloading. In this paper, we investigate MEC scheme considering multiple devices one server. facilitate partitioning, first...
The pregnane X receptor (PXR), or nuclear (NR) 1I2, is a ligand-activated NR superfamily member that enriched in liver and intestine mammals. Activation of PXR regulates the expression genes encoding key proteins involved drug metabolism, efflux, transport. Recent mechanistic investigations reveal post-translational modifications (PTMs), such as phosphorylation, play critical role modulating bimodal function PXR-mediated transrepression transactivation target gene transcription. Upon ligand...
Nonalcoholic fatty liver disease (NAFLD) is characterized by the accumulation of lipid droplets in hepatocytes. NAFLD development and progression associated with an increase hepatic cholesterol levels decreased autophagy lipophagy flux. Previous studies have shown that expression lysosomal acid lipase (LAL), encoded gene LIPA, which can hydrolyze both triglyceride cholesteryl esters, inversely correlated severity NAFLD. In addition, ablation LAL activity results profound Based on this, we...
Pregnane x Receptor (PXR, NR1I2) was initially described as a master‐regulator of drug‐inducible xenobiotic detoxification pathways in the enterohepatic system. However, there is increasing recognition that PXR activation has multiplicity ‘non‐canonical’ roles including anti‐inflammatory effects, alterations glucose and lipid homeostasis, development cancer therapy resistance, driving tumor progression. There clear post‐translational modifications (PTMs) play pivotal role regulating its...
Organelle interactions play a significant role in compartmentalizing metabolism and signaling. Lipid droplets (LDs) interact with numerous organelles including mitochondria, which is largely assumed to facilitate lipid transfer catabolism. However, quantitative proteomics of hepatic peridroplet (PDM) cytosolic mitochondria (CM) revealed that CM are enriched proteins comprising various oxidative pathways whereas PDM involved anabolism. Isotope tracing super resolution imaging confirmed fatty...
Non-alcoholic fatty liver disease (NAFLD) is characterized by the accumulation of lipid droplets (LD) in hepatocytes. If untreated, NAFLD can progress to non-alcoholic steatohepatitis (NASH), cirrhosis, or hepatocellular carcinoma. The progression NASH associated with increases cholesterol levels and decreased autophagy lipophagy flux. Previous studies have shown that expression lysosomal acid lipase (LAL), which hydrolyze both triglyceride cholesteryl esters, inversely correlates severity...