A5087797126- Microbial Natural Products and Biosynthesis
- Synthetic Organic Chemistry Methods
- Carbohydrate Chemistry and Synthesis
- Genomics and Phylogenetic Studies
- Marine Sponges and Natural Products
- Phytochemistry and Bioactive Compounds
- Natural product bioactivities and synthesis
- Cancer therapeutics and mechanisms
- Natural Compounds in Disease Treatment
- Oxidative Organic Chemistry Reactions
- Biochemical and Molecular Research
- Biochemical and Structural Characterization
- Phytochemistry and Biological Activities
- Fungal Biology and Applications
- Chemical Synthesis and Analysis
- Enzyme Catalysis and Immobilization
- Axial and Atropisomeric Chirality Synthesis
- Phytochemistry and biological activities of Ficus species
- Synthesis and Biological Activity
- Cancer-related gene regulation
- Amino Acid Enzymes and Metabolism
- Fungal Plant Pathogen Control
- Synthesis of Indole Derivatives
- Plant-Microbe Interactions and Immunity
- Phytochemical Studies and Bioactivities
Chinese Academy of Sciences
2010-2024
Institute of Microbiology
2021-2024
Henan Provincial People's Hospital
2023-2024
Zhengzhou University
2023-2024
Microbiology Institute of Shaanxi
2022-2024
University of Bristol
2017-2023
State Key Laboratory of Microbial Resources
2022
Shanghai Research Institute of Chemical Industry
2021
At Bristol
2017
Shanghai Institute of Materia Medica
2010-2017
Significance Benzenediol lactone (BDL) polyketides are privileged structures whose various members bind to distinct receptors or modulate the heat shock response and immune system. BDLs biosynthesized by collaborating polyketide synthase enzyme pairs in fungi. Coexpressing random heterocombinations of these enzymes from different BDL biosynthetic pathways yeast cells is shown here lead one-pot, one-step combinatorial biosynthesis structurally diverse practical amounts. Combinatorial promises...
To tackle the growing problem of antibiotic resistance, it is essential to identify new bioactive compounds that are effective against resistant microbes and safe use. Natural products their derivatives are, will continue be, an important source these molecules. Sea sponges harbour a diverse microbiome co-exists with sponge, bacterial communities produce rich array metabolites for protection resource competition. For reasons, sponge microbiota constitutes potential clinically relevant...
The ambruticins are a family of potent antifungal polyketide derived natural products isolated from the myxobacterium Sorangium cellulosum. Their unusual structures include trisubstituted cyclopropyl group and two oxygen heterocycles, tetrahydropyran (THP) dihydropyran (DHP). Herein we report flexible modular approach for total synthesis which is used to prepare F S as well in first 20,21-dihydroambruticin F. strategy unites 3 fragments via Julia-Kocienski olefinations provides important...
Abstract Mupirocin is a clinically important antibiotic produced by trans ‐AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens . The major bioactive metabolite, pseudomonic acid A (PA−A), assembled on tetrasubstituted tetrahydropyran (THP) core incorporating 6‐hydroxy group proposed to be introduced α‐hydroxylation of the thioester acyl carrier protein (ACP) bound chain. Herein, we describe an vitro approach combining purified enzyme components, chemical synthesis, isotopic...
(M)-Bicelaphanol A (1) and (P)-bicelaphanol (2), two unprecedented dimeric trinorditerpenes existing as atropisomers, together with their monomer celaphanol (3), were isolated from the root bark of Celastrus orbiculatus. The structures absolute configurations 1 2 determined by spectroscopic single-crystal X-ray diffraction analyses. Compound exhibited a significant in vitro neuroprotective effect against hydrogen peroxide-induced cell viability decrease PC12 cells at μM, while compounds 3...
Abstract The delineation of the complex biosynthesis potent antibiotic mupirocin, which consists a mixture pseudomonic acids (PAs) isolated from Pseudomonas fluorescens NCIMB 10586, presents significant challenges, and timing mechanisms several key transformations remain elusive. Particularly intriguing are steps that process linear backbone initial polyketide assembly phase to generate first cyclic intermediate PA‐B. These include epoxidation as well incorporation tetrahydropyran (THP) ring...
A multidisciplinary approach combining natural product degradation, fragment synthesis, bioinformatics and NMR spectroscopy was used.
Abstract Thiomarinol and mupirocin are assembled on similar polyketide/fatty acid backbones exhibit potent antibiotic activity against methicillin‐resistant Staphylococcus aureus (MRSA). They both contain a tetrasubstituted tetrahydropyran (THP) ring that is essential for biological activity. Mupirocin mixture of pseudomonic acids (PAs). Isolation the novel compound P, which contains 7‐hydroxy‐6‐keto‐substituted THP, from ΔmupP strain chemical complementation experiments confirm first step...
Where the sea meets land: mupirocin biosynthetic gene cluster (BGC) from terrestrial bacterium <italic>Pseudomonas fluorescens</italic> was repurposed <italic>via</italic> a plug-and-play approach with heterologous genes marine strain that produces thiomarinol.
The presence of β-branches in the structure polyketides that possess potent biological activity underpins widespread importance this structural feature. Kalimantacin is a polyketide antibiotic with selective against staphylococci, and its biosynthesis involves unprecedented incorporation three different sequential β-branching modifications. We use purified single multi-domain enzyme components kalimantacin biosynthetic machinery to address vitro how pattern controlled. Robust discrimination...
Liquid chromatography-photodiode array detector-mass spectrometry-based chemical investigation of the leaves and stems Premna fulva yielded one new iridoid glycoside (1), triterpenoid (2) along with six known compounds isolated for first time from genus. Their structures were established on basis extensive spectroscopic data analyses methods.
Introduction: Accumulating evidence has disclosed that IgA nephropathy (IgAN) could present shortly after the second dose of COVID-19 mRNA vaccine. However, undying mechanism remains unclear and we aimed to investigate potential molecular mechanisms. Methods: We downloaded gene expression datasets vaccination (GSE201535) IgAN (GSE104948). Weighted Gene Co-Expression Network Analysis (WGCNA) was performed identify co-expression modules related IgAN. Differentially expressed genes (DEGs) were...
Further phytochemical investigation of the root bark Periploca sepium afforded nine new spiro-orthoester group-containing pregnane-type glycosides termed periplosides O-V and 3-O-formyl-periploside A. The structures these along with absolute configuration unique seven-membered formyl acetal-bridged function 4,6-dideoxy-3-O-methyl-Δ3-2-hexosulosyl moiety were elucidated on basis spectroscopic data interpretation chemical transformation. configurations major compounds C F established by...
Mupirocin, a commercially available antibiotic produced by Pseudomonas fluorescens NCIMB 10586, and thiomarinol, isolated from the marine bacterium Pseudoalteromonas sp. SANK 73390, both consist of polyketide-derived monic acid homologue esterified with either 9-hydroxynonanoic (mupirocin, 9HN) or 8-hydroxyoctanoic (thiomarinol, 8HO). The mechanisms formation these deceptively simple 9HN 8HO fatty moieties in mup tml, respectively, remain unresolved. To define starter unit generation,...
The total synthesis of ambruticin J and epoxidation/cyclisation studies on model unsaturated hydroxy esters are described.
Abstract A new sulfur-containing spiroketal glycoside, breynin I (1), and a terpenic breyniaionoside E (2), together with 10 known compounds, were isolated from the aerial parts of Breynia vitis-idaea (Euphorbiaceae), traditional Chinese medicine used for treatment chronic bronchitis wounds. Their structures elucidated on basis spectroscopic analysis modified Mosher's method. Keywords: Euphorbiaceaesulfur-containing sesquiterpene glycosideterpenic glycoside Acknowledgements This work was...
Abstract The mupirocin trans- AT polyketide synthase pathway, provides a model system for manipulation of antibiotic biosynthesis. Its final phase involves removal the tertiary hydroxyl group from pseudomonic acid B, PA-B, producing fully active PA-A in complex series steps. To further clarify requirements this conversion, we fed extracts containing PA-B to mutants producer strain singly deficient each mup gene. This additionally identified mupM and mupN as required plus sequence but not...
Abstract The presence of β‐branches in the structure polyketides that possess potent biological activity underpins widespread importance this structural feature. Kalimantacin is a polyketide antibiotic with selective against staphylococci, and its biosynthesis involves unprecedented incorporation three different sequential β‐branching modifications. We use purified single multi‐domain enzyme components kalimantacin biosynthetic machinery to address vitro how pattern controlled. Robust...