A5084368187- Alzheimer's disease research and treatments
- Protein Interaction Studies and Fluorescence Analysis
- Porphyrin and Phthalocyanine Chemistry
- Molecular Sensors and Ion Detection
- Supramolecular Self-Assembly in Materials
- Metal-Catalyzed Oxygenation Mechanisms
- Computational Drug Discovery Methods
- Porphyrin Metabolism and Disorders
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Advanced biosensing and bioanalysis techniques
- Photodynamic Therapy Research Studies
- Advanced Fluorescence Microscopy Techniques
- Nanoplatforms for cancer theranostics
- Bacterial biofilms and quorum sensing
- Protein Structure and Dynamics
- Drug Transport and Resistance Mechanisms
- Synthesis and biological activity
- Probiotics and Fermented Foods
- ATP Synthase and ATPases Research
- Electrochemical Analysis and Applications
- Radioactive element chemistry and processing
- Cellular transport and secretion
- Luminescence and Fluorescent Materials
- Cholinesterase and Neurodegenerative Diseases
National Academy of Sciences of Ukraine
2014-2024
Centre for Social Innovation
2024
Friedrich-Alexander-Universität Erlangen-Nürnberg
2024
Institute of Molecular Biology and Genetics
2014-2023
Palladin Institute of Biochemistry
2021-2022
Institute of General and Inorganic Chemistry
2018
Abstract Amyloid fibrils are insoluble protein aggregates whose accumulation in cells and tissues is connected with a range of pathological diseases. We studied the impact 2 metal complexes (axially coordinated Hf phthalocyanine iron (II) clathrochelate) on aggregation insulin lysozyme. For both proteins, host‐guest interaction these compounds changes kinetics fibrillization affects morphology final aggregates. The very efficient inhibitor fibrillization; its presence, only low amounts...
Abstract We have studied spectral-luminescent properties of the monomethine cyanine dyes both in their free states and presence either double-stranded deoxyribonucleic acids (dsDNAs) or single-stranded ribonucleic (RNAs). The possess low fluorescence intensity an unbound state, which is increased up to 479 times nucleic acids. In RNAs, increase was stronger than that observed dsDNA. Next, we performed staining live fixed cells by all prepared dyes. proved be cell nuclear membrane permeant....
Green-emitting water-soluble amino-ketoenole dye AmyGreen is proposed as an efficient fluorescent stain for visualization of bacterial amyloids in biofilms and the detection pathological vitro. This almost non-fluorescent solution, displays strong green emission presence amyloid fibril proteins. also weakly to biomolecules that are components cells, extracellular matrix or medium: nucleic acids, polysaccharides, lipids, Thus, luminescence turn-on behavior can be utilized biofilm matrix....
Functionalized β-ketoenoles for efficient fluorescence sensing of protein amyloid fibrils giving strong emission increase up to 0.5 QY are designed.
Benzothiazole based cyanine dyes with bridged groups in the pentamethine chain were studied as potential far-red fluorescent probes for protein detection. Spectral-luminescent properties characterized unbound and presence of serum albumins (bovine (BSA), human (HSA), equine (ESA)), globular proteins (β-lactoglobulin, ovalbumin). We have observed that addition leads to a significant increase fluorescence intensity. However, response other was notably lower. The value quantum yield dye bearing...
A fluorescein-tagged iron(ii) cage complex was obtained in a moderate total yield using two-step synthetic procedure starting from its propargylamine-containing clathrochelate precursor. An 11-fold decrease fluorescence quantum is observed passing the given fluorescein-based dye to derivative. excitation energy transfer terminal fluorescent group of macrobicyclic molecule quasiaromatic highly π-conjugated framework can explain this effect. The kinetics hydrolysis acetyl groups acetylated...
Keywords: amyloid fibril, phthalocyanines, inhibitors of fibril formation, insulin, aggregation fluorescent dye
Abstract Amyloid fibrils are rigid β‐pleated protein aggregates that connected with series of harmful diseases and at the same time promising as base for novel nanomaterials. Thus, design compounds able to inhibit or redirect those formation is important both biomedical aims nanotechnology applications. Here, we studied effect tetraphenylporphyrins (metal free, their Cu Pd complexes, functionalized by carboxy amino groups on periphery) insulin amyloid self‐assembling. The strongest impact...
Amyloid fibrils are widely studied both as target in conformational disorders and basis for the development of protein-based functional materials. The three Zr phthalocyanines bearing dehydroacetic acid residue (PcZr(L1)2) its condensed derivatives (PcZr(L2)2 PcZr(L3)2) out-of-plane ligands were synthesized their influence on insulin fibril formation was by amyloid-sensitive fluorescent dye based assay, scanning electron microscopy, absorption spectroscopies. presence shown to modify...
We propose symmetrical cationic trimethine cyanine dyes with β-substituents in the polymethine chain based on modified benzothiazole and benzoxazole heterocycles as probes for detection visualization of live fixed cells by fluorescence microscopy. The spectral-luminescent properties cyanines have been characterized free presence nucleic acids (NA) globular proteins. studied are low to moderate fluorescent when free, but NA, they show an increase emission intensity up 111 times; most...
Abstract Formation of the deposits protein aggregates—amyloid fibrils in an intracellular and intercellular space—is common to a large group amyloid‐associated disorders. Among approaches develop therapy such disorders is use agents preventing fibrillization. Polyaromatic complexes—porphyrins phthalocyanines—are known as compounds possessing anti‐fibrillogenic activity. Here, we explore impact related macrocyclic complexes—phthalocyanines (Pc) octaphenyl porphyrazines (Pz) Mg Zn—on...
Abstract The activity of the boron cluster ‐ closo ‐borates in protein amyloid fibrillization was first studied. For this, we explored effect dianionic compound [B 12 H ] 2− on aggregation amyloidogenic insulin by circular dichroism spectroscopy, amyloid‐sensitive fluorescent dye based assay and transmission electron microscopy. It shown that presence ‐borate during reaction speeds up loss α‐helical structure. This process is concentration‐dependent. C loso at low concentration (10 mM)...
Keywords: fluorescent probes, styrylcyanine dyes, synthesis, regression model QSAR, amyloid fibrils, protein detection
The applicability of surface-enhanced Raman scattering (SERS) on silver nanoparticles for characterization interaction between the phthalocyanine molecule and amyloid fibril was studied using axially coordinated Hf dichloride fibrils amyloidogenic protein insulin. SERS spectra were obtained; analytical enhancement factor estimated to be in range from 4 6 × 104. An intensity its caused by presence fibrillar insulin observed. Deviation this with change concentration manifested.
Red fluorescent dyes are usually charged, lipophilic molecules with relatively high molecular weight, which tend to localize in specific intracellular locations, e. g., a cyanine dye Cy5 is biased towards mitochondria. They often used as markers of biomolecules including nucleic acids and proteins. Since the weight much smaller than that biomolecules, labelling has negligible effect on properties biomolecules. In contrast, conjugation low (pro)drugs can dramatically alter their properties....
The Knoevenagel condensation reaction between dehydracetic acid and aromatic aldehydes is described in this work. carried out directly dehydroacetic the presence of organic bases. optimal conditions for based on various were determined. Twenty-one chalcones with substituents different nature synthesized. composition structure obtained compounds All characteristic signals are present 1H NMR spectra registered CDCl3 DMSO-d6: OH groups range 18.7–16.5 ppm, CH proton – 6.3–5.9 methyl group pyran...
Keywords: squaraine dyes, serum albumins, fluorescent spectroscopy, absorptions detection, fluorescence microscopy
The article is devoted to methods of synthesis, the structure, and spectral characteristics zirconium hafnium phthalocyaninates with out-of-plane coordinated ligands. general scheme for synthesis Zr Hf phthalocyanines includes obtaining initial dichloride or dihydroxo complexes, which then undergo substitution reactions -dicarbonyl compounds, hydroxybenzoic, sulfo- aliphatic carboxylic acids, etc. In case polyphenols, are bidentate ligands, one ligand central atom macrocycle. If compounds...