A5013546354- PI3K/AKT/mTOR signaling in cancer
- Neurological diseases and metabolism
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Porphyrin Metabolism and Disorders
- RNA Research and Splicing
- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Protein Kinase Regulation and GTPase Signaling
- Medical Imaging and Pathology Studies
- Metabolism and Genetic Disorders
- Parathyroid Disorders and Treatments
- RNA regulation and disease
- Cell Adhesion Molecules Research
- Radiopharmaceutical Chemistry and Applications
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- vaccines and immunoinformatics approaches
- Mitochondrial Function and Pathology
- Fungal and yeast genetics research
- Biochemical and Molecular Research
- Bacterial Genetics and Biotechnology
- Proteoglycans and glycosaminoglycans research
Institute of Molecular Biology and Genetics
2015-2024
National Academy of Sciences of Ukraine
2011-2024
Duke University
2024
Oregon Health & Science University
2024
University College London
2006-2024
Andijan State Medical Institute
2012
Kagawa University
2012
Ludwig Cancer Research
2005
Instituto de Biomedicina y Genética Molecular de Valladolid
2003
UConn Health
1994
A novel ribosomal S6 kinase, termed p70 kinase β (p70β), which has a highly conserved amino acid sequence compared with that of p70/p85 (p70α) within the catalytic, extension, and autoinhibitory pseudosubstrate domains, was identified. However, p70β differs from p70α in noncatalytic amino-terminal region carboxyl-terminal tail, contains proline-rich region. The majority regulatory phosphorylation sites identified are p70β. Two isoforms p70β, referred to as β1 (495 acids) β2 (482 acids),...
Coenzyme A (CoA) is an obligatory cofactor in all branches of life. CoA and its derivatives are involved major metabolic pathways, allosteric interactions the regulation gene expression. Abnormal biosynthesis homeostasis have been associated with various human pathologies, including cancer, diabetes neurodegeneration. Using anti-CoA monoclonal antibody mass spectrometry, we identified a wide range cellular proteins which modified by covalent attachment to cysteine thiols (CoAlation). We show...
In all living organisms, coenzyme A (CoA) is an essential cofactor with a unique design allowing it to function as acyl group carrier and carbonyl-activating in diverse biochemical reactions. It synthesized highly conserved process prokaryotes eukaryotes that requires pantothenic acid (vitamin B5), cysteine ATP. CoA its thioester derivatives are involved major metabolic pathways, allosteric interactions the regulation of gene expression. novel unconventional redox has been recently...
Aurora A kinase is a master mitotic regulator whose functions are controlled by several regulatory interactions and post-translational modifications. It frequently dysregulated in cancer, making inhibition very attractive antitumor target. However, recently uncovered links between A, cellular metabolism redox regulation not well understood. In this study, we report novel mechanism of the response to oxidative stress through CoAlation. combination biochemical, biophysical, crystallographic...
NY-CO-58/KIF2C has been identified as a tumor antigen by screening antibody responses in patients with colorectal cancer. However, expression had not consequently examined, and nothing was known about its ability to induce spontaneous T cell responses, which have suggested play role the development of We analyzed 5 cancer lines, samples adjacent healthy tissues from 176 epithelial cancers for RT-PCR Western Blot. 43 35 donors were evaluated ELISpot following stimulation 30mer peptides or...
The metastasis suppressor protein NME1 is an evolutionarily conserved and multifunctional enzyme that plays important role in suppressing the invasion of tumour cells. nucleoside diphosphate kinase (NDPK) activity well recognized balancing intracellular pools nucleotide diphosphates triphosphates to regulate cytoskeletal rearrangement cell motility, endocytosis, trafficking, metastasis. In addition, was found function as a protein-histidine kinase, 3′-5′ exonuclease geranyl/farnesyl...
CoA synthase mediates the last two steps in sequence of enzymatic reactions, leading to biosynthesis. We have recently identified cDNA for and demonstrated that it encodes a bifunctional enzyme possessing 4'-phosphopantetheine adenylyltransferase dephospho-CoA kinase activities. Molecular cloning provided us with necessary tools study subcellular localization regulation this enzyme. Transient expression studies confocal microscopy allowed demonstrate full-length is associated mitochondria,...
The ribosomal protein S6 kinase (S6K) belongs to the AGC family of Ser/Thr kinases and is known be involved in regulation synthesis G(1)/S transition cell cycle. There are two forms S6K, termed S6Kalpha S6Kbeta, which have cytoplasmic nuclear splice variants. Nucleocytoplasmic shuttling has been recently proposed for S6Kalpha, based on use export inhibitor, leptomycin B. However, molecular mechanisms regulating subcellular localization S6Ks response mitogenic stimuli remain elucidated. Here...
Peroxiredoxins (Prdxs) are antioxidant enzymes that catalyse the breakdown of peroxides and regulate redox activity in cell. Peroxiredoxin 5 (Prdx5) is a unique member Prdxs, which displays wider subcellular distribution substrate specificity exhibits different catalytic mechanism when compared to other members family. Here, role key metabolic integrator coenzyme A (CoA) modulating Prdx5 was investigated. We report for first time novel mode regulation mediated via covalent reversible...
Coenzyme A (CoA) is an essential cofactor in all living cells which plays critical role cellular metabolism, the regulation of gene expression and biosynthesis major constituents. Recently, CoA was found to function as a antioxidant both prokaryotic eukaryotic cells. This unconventional mediated by novel post-translational modification, termed protein CoAlation. review will highlight history this discovery, current knowledge, future directions on studying molecular mechanisms CoAlation...
Coenzyme A functions as a carrier of acetyl and acyl groups in living cells is essential for numerous biosynthetic, energy-yielding, degradative metabolic pathways. There are five enzymatic steps CoA biosynthesis. To date, molecular cloning enzymes involved the biosynthetic pathway mammals has been only reported pantothenate kinase. In this study, we present cDNA functional characterization synthase. It an open reading frame 563 aa encodes protein ∼60 kDa. Sequence alignments suggested that...
Coenzyme A (CoA) is a key cellular metabolite known for its diverse functions in metabolism and regulation of gene expression. CoA was recently shown to play an important antioxidant role under various stress conditions by forming disulfide bond with proteins, termed CoAlation. Using anti-CoA antibodies liquid chromatography tandem mass spectrometry (LC-MS/MS) methodologies, CoAlated proteins were identified from organisms/tissues/cell-lines conditions. In this study, we integrated currently...
Abstract Purpose: NY-BR-1 is a recently isolated differentiation antigen, which expressed in normal mammary tissue and breast cancer. However, current data are based on RT-PCR analysis nothing known about the presence of protein level. We previously generated monoclonal antibody to study expression NY-BR-1. Methods: In our immunohistochemical study, was analyzed tissues, various tumor types, 124 primary cancers, 37 paired lymph node metastases. Results: Among present solely ductal epithelium...
Autoantibodies, which are produced against tumor-associated antigens, potential tumor markers and attract a growing interest for cancer detection, differential diagnostics prognosis.To evaluate the diagnostic significance of 40 antigens identified by immunoscreening cDNA libraries from thyroid colon cancers allogenic screening with different types patients' sera.Plaque-spot serological assay.Increased frequency antibody response in sera patients compared that healthy donors was shown toward...
Aim. Generation of monoclonal antibodies specifi c to Coenzyme A. Methods.Hybridoma technique.KLH carrier protein conjugated with CoA was used for immunization.Screening positive clones performed BSA CoA.Results.Monoclonal antibody that cally recognizes and derivatives, but not its precursors ATP cysteine has been generated.Conclusion.In this study, we describe the fi rst time production characterization against CoA.The 1F10 shown recognize in Western blotting, ELISA...