A5086434840- DNA Repair Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Microtubule and mitosis dynamics
- Monoclonal and Polyclonal Antibodies Research
- Telomeres, Telomerase, and Senescence
- Genetic Neurodegenerative Diseases
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Advanced Biosensing Techniques and Applications
- Immunotherapy and Immune Responses
- Medical Imaging and Pathology Studies
- Renal and related cancers
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Glycosylation and Glycoproteins Research
- vaccines and immunoinformatics approaches
- Fungal and yeast genetics research
- Genetic factors in colorectal cancer
- Caveolin-1 and cellular processes
Harvard University
2024
University of Geneva
2014-2021
Taras Shevchenko National University of Kyiv
2011-2012
Institute of Molecular Biology and Genetics
2012
The Rif1 protein, originally identified as a telomere-binding factor in yeast, has recently been implicated DNA replication control from yeast to metazoans. Here, we show that budding protein inhibits activation of prereplication complexes (pre-RCs). This inhibitory function requires two N-terminal motifs, RVxF and SILK, associated with recruitment PP1 phosphatase (Glc7). In G1 phase, both Glc7 interacts an RVxF/SILK-dependent manner proteins pre-RC activation, Mcm4 Sld3, display increased...
Rap1-interacting factor 1 (Rif1) was originally identified in the budding yeast Saccharomyces cerevisiae as a telomere-binding protein that negatively regulates telomerase-mediated telomere elongation. Although this function is conserved distantly related fission Schizosaccharomyces pombe, recent studies, both yeasts and metazoans, reveal Rif1 also functions more globally, temporal control of DNA replication repair. proteins are large characterized by N-terminal HEAT repeats, predicted to...
The Rif1 protein is a negative regulator of DNA replication initiation in eukaryotes. Here we show that budding yeast inhibits at the rDNA locus. Absence Rif1, or disruption its interaction with PP1/Glc7 phosphatase, leads to more intensive replication. effect Rif1-Glc7 on similar Sir2 deacetylase, and two would appear act same pathway, since rif1Δ sir2Δ double mutant shows no further increase Loss activity also accompanied by an repeat instability again not additive sir2Δ. We find,...
Replication forks temporarily or terminally pause at hundreds of hard-to-replicate regions around the genome. A conserved pair budding yeast replisome components Tof1-Csm3 (fission Swi1-Swi3 and human TIMELESS-TIPIN) act as a "molecular brake" promote fork slowdown proteinaceous replication barriers (RFBs), while accessory helicase Rrm3 assists in removing protein obstacles. Here we show that complex promotes pausing independently by recruiting topoisomerase I (Top1) to replisome....
Autoantibodies, which are produced against tumor-associated antigens, potential tumor markers and attract a growing interest for cancer detection, differential diagnostics prognosis.To evaluate the diagnostic significance of 40 antigens identified by immunoscreening cDNA libraries from thyroid colon cancers allogenic screening with different types patients' sera.Plaque-spot serological assay.Increased frequency antibody response in sera patients compared that healthy donors was shown toward...
Medullary breast carcinoma (MBC) despite anaplastic features and high grade has a good prognosis that can be related to prominent lymphocytic infiltration. We analyzed the frequency of antibody response toward 41 SEREX (serological recombinant expression cloning)-defined MBC antigens in sera allogeneic patients using serological plaque-spot assay examined mRNA profile some tissues. This preliminary study allowed us select 18 autoantigens as potential MBC-associated antigens. Further studies...
ABSTRACT Replication forks temporarily or terminally pause at hundreds of hard-to-replicate regions around the genome. A conserved pair budding yeast replisome components Tof1-Csm3 (fission Swi1-Swi3 and human TIMELESS-TIPIN) acts as a ‘molecular brake’ promotes fork slowdown proteinaceous replication barriers (RFBs), while accessory helicase Rrm3 assists in removing protein obstacles. Here we show that complex pausing independently by recruiting topoisomerase I (Top1) to replisome....
Abstract When cells enter mitosis with under-replicated DNA, sister chromosome segregation is compromised, which can lead to massive genome instability. The replisome-associated E3 ubiquitin ligase TRAIP mitigates this threat by ubiquitylating the CMG helicase in mitosis, leading disassembly of stalled replisomes, fork cleavage, and restoration structure alternative end-joining. Here, we show that replisome requires phosphorylation mitotic Cyclin B-CDK1 kinase, as well TTF2, a SWI/SNF ATPase...
Aim. To investigate gene expression profile of human medullary breast carcinoma's tumor-associated antigens in different histological types tumors. analyze à correlation between alterations level and induction immune response against the cor responding antigens. Methods. Real-time polymerase chain reaction. Results. Differential 6 (RAD50, HMGN2, RBPJ, PABPC4, BRAP, DEK) carcinoma have been revealed tumors types. Correlation changes has not found. Conclusions. The genes with differential...