A5016070188- Liver physiology and pathology
- Pediatric Hepatobiliary Diseases and Treatments
- Drug Transport and Resistance Mechanisms
- Liver Disease Diagnosis and Treatment
- Liver Diseases and Immunity
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Liver Disease and Transplantation
- Organ Transplantation Techniques and Outcomes
- Neuropeptides and Animal Physiology
- Parkinson's Disease Mechanisms and Treatments
- Neurological disorders and treatments
- Botulinum Toxin and Related Neurological Disorders
- Pancreatic function and diabetes
- Genetic and Kidney Cyst Diseases
- Polyamine Metabolism and Applications
- Digestive system and related health
- Pancreatitis Pathology and Treatment
- Circadian rhythm and melatonin
- Diet, Metabolism, and Disease
- Renal and related cancers
- Mast cells and histamine
- Biomedical Research and Pathophysiology
- Amino Acid Enzymes and Metabolism
- Genetics and Neurodevelopmental Disorders
- Neuroendocrine Tumor Research Advances
Sapienza University of Rome
2014-2024
Marche Polytechnic University
2005-2024
Hudson Institute
2023
Texas A&M Health Science Center
2009-2010
Baylor Scott & White Medical Center - Temple
2009-2010
University of L'Aquila
2009
Tohoku University Hospital
2009
Central Texas Veterans Health Care System
2009
Due to renewed interest in the cultivation and production of Italian Cannabis sativa L., we proposed a multi-methodological approach explore chemically biologically both essential oil aromatic water this plant. We reported chemical composition terms cannabinoid content, volatile component, phenolic flavonoid pattern, color characteristics. Then, demonstrated ethnopharmacological relevance plant cultivated Italy as source antioxidant compounds toward large panel enzymes (pancreatic lipase,...
In Parkinson's disease (PD), alpha-synuclein (a-syn) can be detected in biological fluids including saliva. Although previous studies found reduced a-syn total (a-syntotal) concentration saliva of PD patients, no have previously examined salivary oligomers (a-synolig) concentrations or assessed the correlation between a-syntotal, a-synolig and clinical features a large cohort patients. Is well known that exerts crucial neurotoxic effect PD. We collected samples from 60 patients 40 age-...
During bile duct ligation (BDL), the growth of large cholangiocytes is regulated by cyclic adenosine monophosphate (cAMP)/extracellular signal-regulated kinase 1/2 (ERK1/2) pathway and closely associated with increased secretin receptor (SR) expression. Although it has been suggested that SR modulates cholangiocyte growth, direct evidence for secretin-dependent proliferation lacking. wild-type (WT) (SR+/+) or knockout (SR−/−) mice underwent sham surgery BDL 3 7 days. We evaluated expression,...
Abstract Caffeic acid phenethyl ester (CAPE) inhibits the growth of tumor cells and is a known inhibitor nuclear factor kappa beta (NF‐κB), which constitutively active in cholangiocarcinoma (CCH) cells. We evaluated effects CAPE on CCH both vitro vivo. Inhibition NF‐κB DNA‐binding activity was confirmed extracts treated with at 50, 40 20 μM. decreases expression NF‐κB1 (p50) RelA (p65). decreased number but not normal cholangiocytes. Cell cycle decrease seen by PCNA protein BrdU‐positive μM...
In bile duct-ligated (BDL) rats, large cholangiocytes proliferate by activation of cAMP-dependent signaling. Melatonin, which is secreted from pineal gland as well extrapineal tissues, regulates cell mitosis interacting with melatonin receptors (MT1 and MT2) modulating cAMP clock genes. the liver, suppresses oxidative damage ameliorates fibrosis. No information exists regarding role in regulation biliary hyperplasia. We evaluated mechanisms action growth cholangiocytes. normal BDL we...
Melatonin therapy or prolonged exposure to complete darkness reduces biliary hyperplasia and liver fibrosis in bile-duct-ligated (BDL) rats; however, no information exists primary sclerosing cholangitis (PSC). Thus, we aimed determine the therapeutic effects of dark melatonin administration on hepatic multidrug resistance gene 2-knockout (Mdr2-/-) mouse model PSC. levels, mass, fibrosis, angiogenesis miR-200b expression were evaluated wild-type Mdr2-/- mice exposed treatment male patients...
To investigate molecular biomarkers of a-synuclein and tau aggregation, autophagy, inflammation in the saliva de novo Parkinson's disease (PD) patients comparison to healthy subjects (HS), correlate data with clinical features PD patients, order establish whether abnormalities these parameters are associated specific clusters their potential diagnostic power differentiating from HS.We measured total oligomeric a-synuclein, total-tau phosphorylated-tau, microtubule-associated protein light...
Cholangiocarcinomas are cancers that have poor prognosis and limited treatment options. The noncanonical Wnt pathway is mediated predominantly by 5a, which activates a Ca 2+ -dependent involving protein kinase C, or -independent the orphan receptor Ror2 subsequent activation of Jun NH 2 -terminal (JNK). This associated with growth-suppressing effects in numerous cell types. We shown anandamide decreases cholangiocarcinoma growth vitro. Therefore, we determined on tumor vivo using xenograft...
Histamine regulates functions via four receptors (HRH1, HRH2, HRH3, and HRH4). The d-myo-inositol 1,4,5-trisphosphate (IP(3))/Ca(2+)/protein kinase C (PKC)/mitogen-activated protein pathway cholangiocarcinoma growth. We evaluated the role of HRH3 in regulation Expression intrahepatic extrahepatic cell lines, normal cholangiocytes, human tissue arrays was measured. In Mz-ChA-1 cells stimulated with (R)-(alpha)-(-)-methylhistamine dihydrobromide (RAMH), we measured (a) growth, (b) IP(3) cyclic...
Sex hormones regulate cholangiocyte hyperplasia in bile duct-ligated (BDL) rats. We studied whether follicle-stimulating hormone (FSH) regulates proliferation. FSH receptor (FSHR) and expression was evaluated liver sections, purified cholangiocytes, cultures (NRICC). In vivo, normal female male rats were treated with or immediately after BDL antide (a gonadotropin-releasing antagonist blocking secretion) a neutralizing antibody for 1 wk. 1) proliferation sections cholangiocytes 2) changes...
Secretin stimulates ductal secretion by interacting with secretin receptor (SR) activating cyclic adenosine 3′,5′-monophosphate/cystic fibrosis transmembrane conductance regulator/chloride bicarbonate anion exchanger 2 (cAMP⇒CFTR⇒Cl−/HCO Symbol AE2) signaling that is elevated biliary hyperplasia. Cholangiocytes secrete several neuroendocrine factors regulating functions autocrine mechanisms. Melatonin inhibits growth and secretin-stimulated choleresis in cholestatic bile-duct–ligated (BDL)...
Abstract Large, but not small, cholangiocytes (1) secrete bicarbonate by interaction with secretin receptors (SRs) through activation of cystic fibrosis transmembrane regulator (CFTR), Cl − /HCO 3 (apex) anion exchanger 2 (Cl AE2), and adenylyl cyclase (AC)8 (proteins regulating large biliary functions) (2) proliferate in response to bile duct ligation (BDL) cyclic adenosine monophosphate (cAMP) signaling. Small, mitotically dormant are activated during damage D- myo -inositol...