A5057083383- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Pharmacogenetics and Drug Metabolism
- Bacterial Infections and Vaccines
- COVID-19 epidemiological studies
- Genomics and Phylogenetic Studies
- Pneumonia and Respiratory Infections
- Aquaculture disease management and microbiota
- SARS-CoV-2 and COVID-19 Research
- Drug Transport and Resistance Mechanisms
- COVID-19 Clinical Research Studies
- Olfactory and Sensory Function Studies
- Drug-Induced Hepatotoxicity and Protection
- Animal Virus Infections Studies
- Cervical Cancer and HPV Research
- Antimicrobial Peptides and Activities
- Hormonal and reproductive studies
- Streptococcal Infections and Treatments
- Biochemical and Structural Characterization
- Influenza Virus Research Studies
- Cancer therapeutics and mechanisms
- Medical Research and Treatments
- Cancer-related molecular mechanisms research
- COVID-19 and healthcare impacts
- Neonatal and Maternal Infections
GlaxoSmithKline (United States)
2001-2025
Lanzhou University
2024
Lanzhou University Second Hospital
2024
Beijing Anzhen Hospital
2024
Capital Medical University
2024
Xiamen University
2018-2023
Harbin Veterinary Research Institute
2011-2023
Chinese Academy of Agricultural Sciences
2011-2023
Fujian Medical University
2021
People's Hospital of Xinjiang Uygur Autonomous Region
2021
The novel meningococcal serogroup B vaccine (4CMenB, Bexsero®), recently approved in Europe and Australia, may soon be included routine infant immunization schedules, subject to guidance from national or regional recommending bodies. In the development of 4CMenB consistent with other newly introduced vaccines, clinical studies have shown concomitant administration vaccines induces an incremental increase some reactions, including fever. As this hinder acceptability, we examined impact...
The CYP2F enzymes, abundantly expressed in the respiratory tract, are active toward many xenobiotic compounds, including naphthalene (NA). However, precise roles of these enzymes tissue-selective chemical toxicity have been difficult to resolve. A <i>Cyp2f2</i>-null mouse was generated this study by disrupting <i>Cyp2f2</i> fourth exon. Homozygous mice, which had no CYP2F2 expression and showed changes other P450 genes examined, were viable fertile utero lethality or developmental deficits....
Abstract Background The novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, also called 2019-nCoV) causes different morbidity risks to individuals in age groups. This study attempts quantify the age-specific transmissibility using a mathematical model. Methods An epidemiological model with five compartments (susceptible–exposed–symptomatic–asymptomatic–recovered/removed [SEIAR]) was developed based on observed transmission features. Coronavirus disease 2019...
CYP2A5, a mouse cytochrome P450 monooxygenase that shows high similarities to human CYP2A6 and CYP2A13 in protein sequence substrate specificity, is expressed multiple tissues, including the liver, kidney, lung, nasal mucosa. Heterologously CYP2A5 active metabolism of both endogenous substrates, such as testosterone, xenobiotic compounds, nicotine cotinine. To determine biological pharmacological functions vivo, we have generated <i>Cyp2a5</i>-null mouse. Homozygous mice are viable fertile;...
In the respiratory tract and lung tissue, a balanced physiological response is essential for Actinobacillus pleuropneumoniae to survive various types of challenges. ClpP, catalytic core Clp proteolytic complex, involved in stresses regulation biofilm formation many pathogenic bacteria. To investigate role ClpP virulence A. pleuropneumoniae, clpP gene was deleted by homologous recombination, resulting mutant strain S8ΔclpP. The reduced growth S8ΔclpP at high temperatures under several other...
Abstract Antibiotic resistance and the ability to form biofilms of Enterococcus faecalis have compromised choice therapeutic options, which triggered search for new strategies, such as use phage endolysins antimicrobial peptides. However, few studies addressed synergistic relationship between these two promising options. Here, we investigated combination endolysin Ply2660 peptide LL-37 target drug-resistant biofilm-producing E. . In vitro bactericidal assays were used demonstrate efficacy...
The licensed meningococcal serogroup B vaccine, 4CMenB (Bexsero®), contains recombinant membrane proteins (rMenB) and outer vesicles (OMV) of the New Zealand strain. We investigated whether reducing OMV and/or protein content influences immunogenicity reactogenicity in healthy two month-old infants. Six formulations were studied: 4CMenB, rMenB with 0, ¼ or ½ dose a half-dose prelicensure formulation as 4-dose primary/booster series, concomitantly routine vaccines (DTaP-HBV-IPV/Hib 7-valent...
The tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), which is abundant in tobacco smoke, a potent lung procarcinogen. present study was aimed to prove that transgenic expression of human cytochrome P450 2A13 (CYP2A13), known be selectively expressed the respiratory tract and most efficient enzyme for NNK bioactivation vitro, will enhance NNK-induced tumorigenesis mouse lung. Kinetic parameters vitro incidence tumors vivo were determined wild-type,...
Purpose: Recent studies showed circular RNA (circRNA) played important regulatory roles in tumors, including genesis of chemotherapy resistance.In this study, the role circHIPK3 on resistance bladder cancer (BC) will be clarified.Methods: Real-time quantitative PCR was applied to examine expression.The gemcitabine sensitivity and cell proliferation viability were analyzed by Cell Counting Kit-8 assay.Double-stained flow cytometry used detect apoptosis.Results: In BC tissues lines, expression...
Abstract Target heterogeneity and intensity of expression in patient tumors are two many critical factors understanding the clinical potential antibody drug conjugates (ADCs) T cell engagers (TCEs) oncology.1, 2 Despite extensive usage, conventional immunohistochemistry (IHC) is often restricted to a handful markers per section, limiting its capacity identify complex patterns antigen samples. In contrast, high-parameter, spatially resolved, proteomic analysis has not only provide widespread...
ABSTRACT Commercial bacterins for Glässer's disease are widely used the prevention of this caused by Haemophilus parasuis ; however, protective efficacy varies depending on strain and serovar. Bacterial ghosts (BGs) empty bacterial envelopes that, unlike classic bacterins, suffer no denaturing steps during their production. These properties may lead to superior protection. In study, a BG vaccine generated from serovar 5 reference Nagasaki was prepared inoculate piglets. The evaluated...
Tick borne encephalitis (TBE) endemic zones are expanding. We previously evaluated long term persistence of antibody 5 years after the first booster immunization following different primary schedules with polygeline-free inactivated TBE vaccine (TBEvac) in adults and adolescents. Here, we report anti-TBE virus (TBEV) from 6 to 10 post-booster administration.This was a phase IV, open-label, single-center, second extension study (NCT01562444), conducted Czechia. Healthy adolescents ≥12 who had...
The antithyroid drug methimazole (MMZ) can cause severe, tissue-specific toxicity in mouse olfactory mucosa (OM), presumably through a sequential metabolic activation of MMZ by cytochrome P450 (P450) and flavin monooxygenases (FMO). aims this study were to determine whether CYP2A5, one the most abundant enzymes OM, is involved activation, comparing <i>Cyp2a5</i>-null with wild-type (WT) mice, hepatic microsomal enzymes, including are essential for MMZ-induced OM toxicity,...
Actinobacillus pleuropneumoniae is a Gram-negative bacterium and the cause of porcine pleuropneumonia. When encounters nutritional starvation, relA-dependent (p)ppGpp-mediated stringent response activated. The modified nucleotides guanosine 5'-diphosphate 3'-diphosphate (ppGpp) 5'-triphosphate (pppGpp) are known to be signaling molecules in other prokaryotes. Here, investigate role (p)ppGpp A. pleuropneumoniae, we created mutant strain, S8ΔrelA, which lacks (p)ppGpp-synthesizing enzyme RelA,...
Abstract Background A novel coronavirus named as “SARS-CoV-2” has spread widely in many countries since December 2019, especially China. This study aimed to quantify the age-specific transmissibility by using a mathematical model. Methods An susceptible – exposed symptomatic asymptomatic recovered seafood market (SEIARW) model was developed based on two suspected transmission routes (from person and person). The people from Wuhan City were divided into different age groups. We used subscript...
Abstract MicroRNA-200 (miR-200) family is highly expressed in ovarian cancer. We evaluated the levels of members relative to internal control miR-103a cancer and blood specimens collected from American Hong Kong Chinese institutions, as well a laying hen spontaneous model. The miR-200a, miR-200b miR-200c were significantly elevated all human versus samples. Further analyses showed higher miR-200 (except miR-429) miR-200a miR141) samples than their respective counterparts. Subtype-specific...
Background: It is much valuable to evaluate the comparative effectiveness of coronavirus disease 2019 (COVID-19) prevention and control in non-pharmacological intervention phase pandemic across countries identify useful experiences that could be generalized worldwide. Methods: In this study, we developed a susceptible–exposure–infectious–asymptomatic–removed (SEIAR) model fit daily reported COVID-19 cases 160 countries. The time-varying reproduction number ( R t ) was estimated through...