A5084718805- interferon and immune responses
- Immune Response and Inflammation
- Viral Infections and Vectors
- RNA regulation and disease
- Cytokine Signaling Pathways and Interactions
- Herpesvirus Infections and Treatments
- Mosquito-borne diseases and control
- Viral Infections and Immunology Research
- Biofuel production and bioconversion
- Lignin and Wood Chemistry
- Hepatitis B Virus Studies
- Immunotherapy and Immune Responses
- Viral Infections and Outbreaks Research
- Biochemical and biochemical processes
- Thermochemical Biomass Conversion Processes
- Leaf Properties and Growth Measurement
- Ginkgo biloba and Cashew Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Influenza Virus Research Studies
- Endoplasmic Reticulum Stress and Disease
- Genomics, phytochemicals, and oxidative stress
- HIV Research and Treatment
- Optical Polarization and Ellipsometry
- Plant Surface Properties and Treatments
- Textile materials and evaluations
Aarhus University
2018-2025
Kyoto University
2009-2021
Nikon (Japan)
2019
Frontier Medical College
2018
Laboratory of Molecular Genetics
2009
Retinoic acid inducible gene I (RIG-I)-like receptors (RLRs) function as cytoplasmic sensors for viral RNA to initiate antiviral responses including type interferon (IFN) production. It has been unclear how RIG-I encounters and senses RNA. To address this issue, we examined intracellular localization of in response infection using newly generated anti-RIG-I antibody. Immunohistochemical analysis revealed that RLRs localized virus-induced granules containing stress granule (SG) markers...
Herpes simplex encephalitis (HSE) in children has previously been linked to defects type I interferon (IFN) production downstream of Toll-like receptor 3. Here, we describe a novel genetic etiology HSE by identifying heterozygous loss-of-function mutation the IFN regulatory factor 3 (IRF3) gene, leading autosomal dominant (AD) IRF3 deficiency haploinsufficiency, an adolescent female patient with HSE. is activated most pattern recognition receptors recognizing viral infections and plays...
The RIG-I like receptor (RLR) comprises three homologues: (retinoic acid-inducible gene I), MDA5 (melanoma differentiation-associated 5), and LGP2 (laboratory of genetics physiology 2). Each RLR senses different viral infections by recognizing replicating RNA in the cytoplasm. contains a conserved C-terminal domain (CTD), which is responsible for binding specificity to RNAs, including double-stranded (dsRNA) 5'-triphosphated single-stranded (5'ppp-ssRNA). Here, solution structures CTD...
Abstract The cGAS-STING pathway plays a crucial role in anti-tumoral responses by activating inflammation and reprogramming the tumour microenvironment. Upon activation, STING traffics from endoplasmic reticulum (ER) to Golgi, allowing signalling complex assembly induction of interferon inflammatory cytokines. Here we report that cGAMP stimulation leads transient decline ER cholesterol levels, mediated Sterol O-Acyltransferase 1-dependent esterification. This facilitates membrane curvature...
Pyroligneous acids (PA) from hardwood, softwood, and bamboo significantly disinfected encephalomyocarditis virus (EMCV). Twenty-five kinds of phenolic derivatives in the PAs were identified quantified. The total amounts compounds PA is higher than those softwood hardwood. Phenol, 2-methoxyphenol, 2-methoxy-4-methylphenol, 2-methoxy-4-ethylphenol are most abundant examined. activities all against assessed. number hydroxyl groups affects antiviral activity, catechol its exhibit viral...
RIG-I-like receptor (RLR) plays a pivotal role in the detection of invading pathogens to initiate type I interferon (IFN) gene transcription. Since aberrant IFN production is harmful, RLR signaling strictly regulated. However, regulatory mechanisms are not fully understood. By expression cloning, we identified Pumilio proteins, PUM1 and PUM2, as candidate positive regulators RIG-I signaling. Overexpression proteins their knockdown augmented diminished IFN-β promoter activity induced by...
Current therapeutics for hepatitis B virus (HBV) patients such as nucleoside analogs (NAs) are effective; however, new antiviral drugs against HBV still desired. Since the interaction between epsilon (ε) sequence of pregenomic RNA and viral polymerase (Pol) is a key step in replication cycle, we aimed to identify small compounds its inhibition, established pull-down assay system detection ε-RNA-binding-Pol. Screening showed that 5 out 3,965 inhibited ε-Pol binding, identified rosmarinic...
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a tick-borne phlebovirus of the family Bunyaviridae, SFTS virus (SFTSV). Wild-type and type I interferon (IFN-I) receptor 1-deficient (IFNAR1-/-) mice have been established as nonlethal lethal models SFTSV infection, respectively. However, mechanisms IFN-I production in vivo factors causing are not well understood. Using bone marrow-chimeric mice, we found that signaling hematopoietic cells was...
Retinoic acid inducible gene I (RIG-I)-like receptors (RLRs) function as cytoplasmic sensors for viral RNA to initiate antiviral responses including type interferon (IFN) production.It has been unclear how RIG-I encounters and senses RNA.To address this issue, we examined intracellular localization of in response infection using newly generated anti-RIG-I antibody.Immunohistochemical analysis revealed that RLRs localized virus-induced granules containing stress granule (SG) markers together...
CpG-oligodeoxynucleotide (CpG ODN)-based toll-like receptor (TLR) agonists are promising immunostimulatory adjuvants, however, low entry into TLR-rich cellular endosomal compartments and poor lymphatic accumulation limit clinical translation. In this work, we introduce a recombinant human serum albumin (rHA)-CpG ODN covalent conjugate (rHA-CpG) designed to exploit the neonatal Fc (FcRn)-driven sorting pathway maximise CpG delivery TLR9-rich endosomes accumulate in lymph nodes. Site-selective...
Virus pandemics pose a threat to human life. In this study, we developed simple method for the conversion of woody biomass into antiviral substances. Beech wood was decomposed by microwave acidolysis in 0.5% aq H2SO4 and fractionated silica gel chromatography. The obtained fractions exhibited strong activity against encephalomyocarditis virus (EMCV) without cytotoxicity. Structural analyses revealed that separated substances were lignin–carbohydrate complexes with molecular weight range...
Pattern recognition receptors (PRRs) induce host defense but can also exacerbated inflammatory responses. This raises the question of whether other mechanisms are involved in early defense. Using transcriptome analysis disrupted transcripts herpes simplex virus (HSV)-infected cells, we find that HSV infection disrupts hypoxia-inducible factor (HIF) transcription network neurons and epithelial cells. Importantly, HIF activation leads to control replication. Mechanistically, induces autophagy,...
The pyrolysis product, wood vinegar (WV), from Japanese larch exhibited strong antiviral activity against the encephalomycarditis virus (EMCV). Catechol, 3-methyl-, 4-methyl-, 4-ethyl-, and 3-methoxycatechol, 2-methyl-1,4-benzenediol were identified as major compounds. viral inhibition ability of these compounds was affected by structure position substituent group attached to aromatic skeleton. IC50 catechol 0.67 mg mL-1 those its derivatives <0.40 mL-1. Methyl ethyl substitution in para...
Neurotropic viruses, including herpes simplex virus (HSV) types 1 and 2, have the capacity to infect neurons can cause severe diseases. This is associated with neuronal cell death, which may contribute morbidity or even mortality if infection not controlled. However, mechanistic details of HSV-induced death remain enigmatic. Here, we report that lytic HSV-2 human neuron-like SH-SY5Y cells primary murine brain leads mediated by gasdermin E (GSDME). HSV-2-induced GSDME-mediated occurs...
Abstract DNA is a danger signal sensed by cGAS to engage signaling through STING activate innate immune functions. The best-studied downstream responses activation include expression of type I interferon and inflammatory genes, but also activates other pathways, including apoptosis. Here, we report that STING-dependent induction apoptosis in macrophages occurs the intrinsic mitochondrial pathway mediated via IRF3 acts independently gene transcription. By intersecting four mass spectrometry...
Currently, there is no available therapy to eradicate hepatitis B virus (HBV) in chronically infected individuals. This due the difficulty eliminating viral covalently closed circular (ccc) DNA, which central gene expression and replication of HBV. We developed an assay system for nuclear DNA using integration-deficient lentiviral vector. vector produced non-integrated nuclei cells. engineered this encode firefly luciferase monitor episome DNA. screened 3,840 chemicals by luciferase-reducing...
The mechanisms underlying susceptibility to recurrent herpes simplex virus type 2 (HSV-2) meningitis remain incompletely understood. In a patient experiencing multiple episodes of HSV-2 meningitis, we identified monoallelic variant in the IKBKE gene, which encodes IKKε kinase involved induction antiviral IFN genes. Patient cells displayed impaired IFN-β1 (IFNB1) expression upon infection with or stimulation double-stranded DNA (dsDNA) and failed induce phosphorylation STING, an activation...
Early detection of cancer through medical imaging has a critical impact on patient survival rates. There are many efforts for detecting early <i>in situ</i> using modalities other than traditional optical imaging, which contain additional information over conventional micrographs surface morphology acquired without staining. We analyzed the Mueller matrix components human colon tissue obtained with an polarimeter microscope at illumination wavelength 442 nm by principal analysis in order to...