A5035238241- Cancer Cells and Metastasis
- Genetic factors in colorectal cancer
- Axon Guidance and Neuronal Signaling
- Cancer, Hypoxia, and Metabolism
- Colorectal Cancer Treatments and Studies
- DNA Repair Mechanisms
- RNA modifications and cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Pluripotent Stem Cells Research
- Hippo pathway signaling and YAP/TAZ
- Bacteriophages and microbial interactions
- Phagocytosis and Immune Regulation
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Colorectal Cancer Screening and Detection
- Metal complexes synthesis and properties
- Liver physiology and pathology
- Immunotherapy and Immune Responses
- Ferroptosis and cancer prognosis
- Sarcoma Diagnosis and Treatment
- Cancer-related gene regulation
- Cancer Genomics and Diagnostics
- Cancer-related Molecular Pathways
- Wnt/β-catenin signaling in development and cancer
Gebze Technical University
2012-2025
Bilkent University
2006-2011
Marmara University
2007
Scientific and Technological Research Council of Turkey
1996-2001
TÜBİTAK Marmara Research Center
1996-1997
Molecular Oncology (United States)
1996
Istanbul University
1994-1995
The epithelial-mesenchymal transition (EMT) contributes to cancer metastasis. Two ZEB family members, ZEB1 and ZEB2(SIP1), inhibit transcription of the E-cadherin gene induce EMT in vitro. However, their relevance human is insufficiently studied. Here, we performed a comparative study SIP1 proteins cell lines one form malignancy, carcinoma bladder. Whereas protein was expressed all E-cadherin-negative lines, being part responsible for high motility bladder cells, hardly ever detectable cells...
Resistance to adjuvant chemotherapy is a major clinical problem in the treatment of colorectal cancer (CRC). The aim this study was elucidate role an epithelial mesenchymal transition (EMT)‐inducing protein, ZEB2, chemoresistance CRC, and uncover underlying mechanism. We performed IHC for ZEB2 association analyses with outcomes on primary CRC matched liver metastases compliance observational biomarker guidelines. expression tumours independent prognostic marker reduced overall survival...
Tumor cells have the capacity to proliferate indefinitely that is qualified as replicative immortality. This ability contrasts with intrinsic control of number cell divisions in human somatic tissues by a mechanism called senescence. Replicative immortality acquired inactivation p53 and p16 INK4a genes reactivation hTERT gene expression. It unknown whether cancer reversible. Here, we show spontaneous induction senescence p53-and -deficient hepatocellular carcinoma cells. phenomenon...
SLIT-ROBO families of proteins mediate axon pathfinding and their expression is not solely confined to nervous system. Aberrant genes was repeatedly shown in a wide variety cancers, yet data about collective behavior hepatocellular carcinoma (HCC) missing. Hence, we quantified transcripts HCC cell lines, normal tumor tissues from liver.Expression family members by real-time qRT-PCR 14 8 35 the liver. ANOVA Pearson's correlation analyses were performed R environment, different...
Smad interacting protein-1 is a transcription factor that implicated in transforming growth factor-β/bone morphogenetic protein signaling and repressor of E-cadherin human telomerase reverse transcriptase. It also involved epithelial-mesenchymal transition tumorigenesis. However, genetic epigenetic alterations SIP1 have not been fully elucidated cancers. In this study, we investigated mutations promoter hypermethylation the gene hepatocellular carcinomas.SIP1 expression was analyzed HCC cell...
Abstract Epithelial‐mesenchymal transition (EMT) is an embryonic program that reactivated in cancer and regulates the invasion metastasis of tumor cells. Zinc finger E‐box binding homeobox 2 (ZEB2) induces EMT by upregulating matrix metalloproteinases (MMP), yet MMP genes lack ZEB2 motif their promoters. Recently, expression MMPs was associated to activation ETS1 transcription factor; however, a link between ETS proto‐oncogene 1, factor (ETS1) remains be elucidated. Hence, we investigated...
<h3>Importance</h3> At present, patients with colorectal cancer (CRC) are risk stratified using TNM histologic features. More recently, an association between a mesenchymal phenotype and high of disease recurrence micrometastases has been recognized. <h3>Objective</h3> To investigate the epithelial to transition (EMT)–inducing transcription factor ZEB2 (zinc finger E box–binding homeobox 2), survival outcomes, efficacy as biomarker when added refinement staging after curative intent surgery...
HCC is a common and lethal malignancy multi-kinase inhibitors (MKIs) are among the therapeutic options for unresectable cases. However, response rates to MKIs remained variable, necessitating identification of predictive biomarkers. Plexin C1 (PLXNC1), receptor involved in cell signaling, has emerged as potential candidate regulate tumor responses. This study aims evaluate impact PLXNC1 expression on sensitivity cells MKI therapy. shRNA-mediated knock-down control clones lines PLC/PRF/5...
Abstract Background One-third of breast cancers display amplifications the ERBB2 gene encoding HER2 kinase receptor. Trastuzumab, a humanized antibody directed against an epitope on subdomain IV extracellular domain is used for therapy HER2-overexpressing mammary tumors. However, many tumors are either natively resistant or acquire resistance Trastuzumab. Antibodies to different epitopes promising candidates replacement combinatorial therapy. For example, Pertuzumab that binds II and...
Hepatocellular carcinoma (HCC) is a common and deadly cancer; however, very little improvement has been made towards its diagnosis prognosis. The expression functional contribution of the receptor tyrosine kinase ROR1 have not investigated in HCC before. Hence, we cells assessed involvement hepatocarcinogenesis.Recombinant bacterial protein was used as an immunogen to generate monoclonal antibodies. transcript levels were detected by RT-qPCR Western blotting using homemade anti-ROR1...
Abstract DNA repair is essential for successful propagation of genetic material and fidelity transcription. Nucleotide excision (NER) one the earliest mechanisms, functionally conserved from bacteria to human. The fact that number NER genes vary significantly between prokaryotes metazoans gives insight proteins have evolved acquire additional functions combat challenges associated with a diploid genome, including being involved in decision apoptosis. However, no direct association apoptosis...
Hereditary non-polyposis colon cancer (HNPCC) is a common hereditary disease characterized by predisposition to an early onset of colorectal cancer. The majority the HNPCC families carry germline mutations either hMSH2 or hMLH1 genes, whereas hPMS1 and hPMS2 genes have rarely been observed. Almost all reported so far concern typical families. However, there are that display aggregations even though they do not fulfil criteria (incomplete families) as well sporadic cases cancers could be...
Background and Aims. Hepatocellular carcinoma is an aggressive malignancy of the liver ranked as sixth most common cancer worldwide. There still room for novel markers to improve diagnosis monitoring HCC. Our observations in databases that PLXNC1 upregulated HCC led us investigate expression profile Plexin C1 mRNA protein cell lines tissues. Methods . A recombinant encompassing part extracellular domain was used antigen monoclonal antibody development. Transcript levels were determined by...
Objective: NETosis, a suicide mechanism of neutrophils, is an important immune defense against pathogens, and when uncontrolled, it contributes to severe tissue damage leading acute lung injury in influenza respiratory syndrome-coronavirus-2 infections.Here we aimed determine whether blocking C-X-C motif chemokine receptors 1/2 (CXCR1/2) by reparixin prevents vitro NETosis induced either recombinant DEK (rDEK), interleukin-8 (rIL-8) (both interacts with CXCR2 receptor) or the serum patients...
N,N′-bis(aniline)glyoxime (H2L, 1) has been prepared from aniline and (E,E)-dichloroglyoxime (DCGO) or (E,E)-dibromoglyoxime (DBGO). The reaction of with solution PtCl2 in dimethylsulfoxide produced brown-colored bis(E,E-dioximato)platinum(II) complex {[(E,E)-Pt(HL)2]} (1a) featuring N-chelating ligand. crystal structure this identified by X-ray diffraction on a single-crystal. In vitro assay proliferation, cell death, DNA-damage uptake activities 1 1a were tested HCC lines, SNU-182 HUH-7....
Early and differential diagnosis of hepatocellular carcinoma (HCC) requires sensitive specific tissue serum markers. On the other hand, proteins involved in tumorigenesis are extensively modulated on exposure to apoptotic stimuli, including ultraviolet (UVC) irradiation. Hence, we generated monoclonal antibodies by using UVC-irradiated cells an HCC cell line, HUH7, aiming explore differentially expressed tumors apoptosis. We obtained 18 hybridoma clones recognizing protein targets HUH7...