A5021252370- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- CRISPR and Genetic Engineering
- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- Advanced Biosensing Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Noise Effects and Management
- Hearing Impairment and Communication
- Viral Infections and Immunology Research
- Influenza Virus Research Studies
- RNA and protein synthesis mechanisms
- Hearing Loss and Rehabilitation
Massachusetts Institute of Technology
2020-2024
Broad Institute
2020-2024
Washington University in St. Louis
2023
Zero to Three
2023
Harvard University
2020
Howard Hughes Medical Institute
2020
Harvard–MIT Division of Health Sciences and Technology
2020
Concord Consortium
2020
The great majority of globally circulating pathogens go undetected, undermining patient care and hindering outbreak preparedness response. To enable routine surveillance comprehensive diagnostic applications, there is a need for detection technologies that can scale to test many samples
The COVID-19 pandemic has highlighted that new diagnostic technologies are essential for controlling disease transmission. Here, we develop SHINE (Streamlined Highlighting of Infections to Navigate Epidemics), a sensitive and specific tool can detect SARS-CoV-2 RNA from unextracted samples. We identify the optimal conditions allow RPA-based amplification Cas13-based detection occur in single step, simplifying assay preparation reducing run-time. improve HUDSON rapidly inactivate viruses...
Abstract The emergence and outbreak of SARS-CoV-2, the causative agent COVID-19, has rapidly become a global concern highlighted need for fast, sensitive, specific tools to surveil circulating viruses. Here we provide assay designs experimental resources, use with CRISPR-based nucleic acid detection, that could be valuable ongoing surveillance. We detection 67 viral species subspecies, including: phylogenetically-related viruses, viruses similar clinical presentation. are outputs algorithms...
Abstract The COVID-19 pandemic has highlighted that new diagnostic technologies are essential for controlling disease transmission. Here, we develop SHINE (SHERLOCK and HUDSON Integration to Navigate Epidemics), a sensitive specific integrated tool can detect SARS-CoV-2 RNA from unextracted samples. We combine the steps of SHERLOCK into single-step reaction optimize accelerate viral inactivation in nasopharyngeal swabs saliva. SHINE’s results be visualized with an in-tube fluorescent readout...
Abstract The COVID-19 pandemic, and the recent rise widespread transmission of SARS-CoV-2 Variants Concern (VOCs), have demonstrated need for ubiquitous nucleic acid testing outside centralized clinical laboratories. Here, we develop SHINEv2, a Cas13-based diagnostic that combines quick ambient temperature sample processing lyophilized reagents to greatly simplify test procedure assay distribution. We benchmarked SHINEv2 detection against state-of-the-art antigen-capture tests using 96...
Abstract The high disease burden of influenza virus poses a significant threat to human health and requires better methods rapidly detect its many circulating species, subtypes, variants. No current diagnostic technology meets the combined critical needs for rapid, sensitive, specific, cost-effective method point-of-need (PON) detection discrimination with minimal equipment requirements. Here, we introduce such using SHINE (Streamlined Highlighting Infections Navigate Epidemics),...
This protocol describes how to perform a SHINE paper-based assay detect SARS-CoV-2 RNA from self-collected saliva sample. is intended for in-home use. All enzymatic components are provided as single-test freeze-dried pellet shelf-stable storage, and all steps of the performed at ambient temperature. The presented here an improved version method in Arizti-Sanz J*, Freije CA*, et al. Integrated sample inactivation, amplification, Cas13-based detection SARS-CoV-2. bioRxiv (2020).
This protocol describes how to perform a SHINE paper-based assay detect SARS-CoV-2 RNA from self-collected nasopharyngeal sample. is intended for in-home use. All enzymatic components are provided as single-test freeze-dried pellet shelf-stable storage, and all steps of the performed at ambient temperature. The presented here an improved version method in Arizti-Sanz J*, Freije CA*, et al. Integrated sample inactivation, amplification, Cas13-based detection SARS-CoV-2. bioRxiv (2020).
This protocol describes how to perform a SHINE in-tube fluorescent assay detect SARS-CoV-2 RNA from self-collected nasopharyngeal sample. is intended for point-of-care use. All enzymatic components are provided as single-test freeze-dried pellet shelf-stable storage, and all steps of the performed at ambient temperature. The requires transilluminator or another equivalent blue light emitting device. presented here an improved version method in Arizti-Sanz J*, Freije CA*, et al. Integrated...
This protocol describes how to perform a SHINE in-tube fluorescent assay detect SARS-CoV-2 RNA from self-collected nasopharyngeal sample. is intended for point-of-care use. All enzymatic components are provided as single-test freeze-dried pellet shelf-stable storage, and all steps of the performed at ambient temperature. The requires transilluminator or another equivalent blue light emitting device. presented here an improved version method in Arizti-Sanz J*, Freije CA*, et al. Integrated...