A5034167178- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Cystic Fibrosis Research Advances
- Microbial Community Ecology and Physiology
- Vibrio bacteria research studies
- Pneumonia and Respiratory Infections
- Inhalation and Respiratory Drug Delivery
- Marine Biology and Environmental Chemistry
- Bacteriophages and microbial interactions
- Antimicrobial Peptides and Activities
- Oral microbiology and periodontitis research
- Bacterial Infections and Vaccines
- Streptococcal Infections and Treatments
- Corrosion Behavior and Inhibition
- Marine Biology and Ecology Research
- Evolutionary Game Theory and Cooperation
- Nosocomial Infections in ICU
- Bacterial Identification and Susceptibility Testing
- Biosensors and Analytical Detection
- Orthopedic Infections and Treatments
- Legionella and Acanthamoeba research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Antimicrobial Resistance in Staphylococcus
- 3D Printing in Biomedical Research
University of Southampton
2016-2025
Diamond Light Source
2023
NIHR Southampton Biomedical Research Centre
2019-2020
University Hospital Southampton NHS Foundation Trust
2012-2020
NIHR Southampton Respiratory Biomedical Research Unit
2011-2018
Mayo Clinic in Florida
2017
Mayo Clinic in Arizona
2017
Royal Brompton Hospital
2016
Southampton General Hospital
2016
National Institute for Health Research
2014-2016
Summary Pseudomonas aeruginosa produces extracellular DNA which functions as a cell‐to‐cell interconnecting matrix component in biofilms. Comparison of and chromosomal by the use polymerase chain reaction Southern analysis suggested that is similar to whole‐genome DNA. Evidence P. biofilms cultures generated via lysis subpopulation bacteria was obtained through experiments where β‐galactosidase released from lacZ ‐containing strains assessed. Experiments with wild type lasIrhlI , pqsA pqsL...
ABSTRACT Bacterial biofilms at times undergo regulated and coordinated dispersal events where sessile biofilm cells convert to free-swimming, planktonic bacteria. In the opportunistic pathogen Pseudomonas aeruginosa , we previously observed that occurs concurrently with three interrelated processes within mature biofilms: (i) production of oxidative or nitrosative stress-inducing molecules inside structures, (ii) bacteriophage induction, (iii) cell lysis. Here examine whether specific...
Most biofilms in their natural environments are likely to consist of consortia species that influence each other synergistic and antagonistic manners. However, few reports specifically address interactions within multispecies biofilms. In this study, 17 epiphytic bacterial strains, isolated from the surface marine alga Ulva australis, were screened for when present together different combinations. Four isolates, Microbacterium phyllosphaerae, Shewanella japonica, Dokdonia donghaensis,...
Bacteria growing in biofilms often develop multicellular, three-dimensional structures known as microcolonies. Complex differentiation within of Pseudomonas aeruginosa occurs, leading to the creation voids inside microcolonies and dispersal cells from these voids. However, key developmental processes regulating events are poorly understood. A normal component multicellular development is cell death. Here we report that a repeatable pattern death lysis occurs P. during course development....
ABSTRACT Bacteria in biofilms often undergo active dispersal events and revert to a free-swimming, planktonic state complete the biofilm life cycle. The signaling molecule nitric oxide (NO) was previously found trigger opportunistic pathogen Pseudomonas aeruginosa at low, nontoxic concentrations (N. Barraud, D. J. Hassett, S. H. Hwang, A. Rice, Kjelleberg, Webb, Bacteriol. 188:7344-7353, 2006). NO further shown increase cell motility susceptibility antimicrobials. Recently, numerous studies...
Strategies to induce biofilm dispersal are of interest due their potential prevent formation and biofilm-related infections. Nitric oxide (NO), an important messenger molecule in biological systems, was previously identified as a signal for biofilms the model organism Pseudomonas aeruginosa. In present study, use NO anti-biofilm agent more broadly assessed. Various donors, at concentrations estimated generate levels picomolar low nanomolar range, were tested on single-species relevant...
Periprosthetic infection (PI) causes significant morbidity and mortality after fixation joint arthroplasty has been extensively linked to the formation of bacterial biofilms. Poly(methyl methacrylate) (PMMA), as a cement or beads, is commonly used for antibiotic release site but displays variable elution kinetics also represents potential nidus infection, therefore requiring surgical removal once antibiotics have eluted. Absorbable cements shown improved wider range and, crucially, complete...
A current question in biofilm research is whether biofilm-specific genetic processes can lead to differentiation physiology and function among cells. In Pseudomonas aeruginosa, phenotypic variants which exhibit a small-colony phenotype on agar media markedly accelerated pattern of development compared that the parental strain are often isolated from biofilms. We grew P. aeruginosa biofilms glass flow cell reactors observed emergence (SCVs) effluent runoff correlated with plaque-forming...
Pseudoalteromonas tunicata is a biofilm-forming marine bacterium that often found in association with the surface of eukaryotic organisms. It produces range extracellular inhibitory compounds, including an antibacterial protein (AlpP) thought to be beneficial for P. during competition space and nutrients on surfaces. As part our studies interactions between epiphytic bacterial community plant Ulva lactuca, we investigated hypothesis superior competitor compared other bacteria isolated from...
Many plants and animals are defended from predation or herbivory by inhibitory secondary metabolites, which in the marine environment very common among sessile organisms. Among bacteria, where there is greatest metabolic potential, little known about chemical defenses against bacterivorous consumers. An emerging hypothesis that bacterial communities organized as biofilms serve refuge predation. By testing growth survival of two nanoflagellates, we find evidence chemically mediated resistance...
In both natural and artificial environments, bacteria predominantly grow in biofilms, often disperse from biofilms as freely suspended single-cells. the present study, formation dispersal of planktonic cellular aggregates, or 'suspended biofilms', by Pseudomonas aeruginosa liquid batch cultures were closely examined, compared to biofilm on a matrix polyester (PE) fibers solid surface cultures. Plankton samples analyzed laser-diffraction particle-size scanning (LDA) microscopy aggregates....
ABSTRACT Significant substratum damage can occur when plasticized PVC (pPVC) is colonized by microorganisms. We investigated microbial colonization of pPVC in an situ, longitudinal study. Pieces containing the plasticizers dioctyl phthalate and adipate (DOA) were exposed to atmosphere for up 2 years. Fungal bacterial populations quantified, colonizing fungi identified rRNA gene sequencing morphological characteristics. Aureobasidium pullulans was principal fungus, establishing itself on...
Bacteria that produce inhibitory compounds on the surface of marine algae are thought to contribute defense host plant against colonization fouling organisms. However, number bacterial cells necessary defend is not known. Pseudoalteromonas tunicata and Phaeobacter sp. strain 2.10 (formerly Roseobacter gallaeciensis) bacteria often found in association with alga Ulva australis a range extracellular common P. biofilms cell densities ranging from 10(2) 10(8) cm(-2) were established polystyrene...
Just say NO to biofilms: NO-donors are used disperse a bacterial biofilm so that co-administered antibiotics will kill the more susceptible unattached cells. The chemically stable cephalosporin-3′-diazeniumdiolate NO-donor prodrug (see scheme) is activated by β-lactamases and facilitates this two-step erradication. Detailed facts of importance specialist readers published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted...
The marine bacterium Pseudoalteromonas tunicata produces an antibacterial and autolytic protein, AlpP, which causes death of a subpopulation cells during biofilm formation mediates differentiation, dispersal, phenotypic variation among dispersal cells. AlpP homologue (LodA) in the Marinomonas mediterranea was recently identified as lysine oxidase cell through production hydrogen peroxide. Here we show that P. also acts peroxide generated is responsible for within microcolonies development...
Abstract Background Interactions between transcription factors and DNA lie at the centre of many biological processes including recombination, replication, repair transcription. Most bacteria encode diverse proteins that act as to regulate various traits. Several technologies for identifying protein–DNA interactions genomic level have been developed. Bind-n-seq is a high-throughput in vitro method first deployed analyse associated with eukaryotic zinc-finger proteins. The has three steps (i)...
ABSTRACT Pseudalteromonas tunicata and Roseobacter gallaeciensis are biofilm-forming marine bacteria that often found in association with the surface of green alga Ulva australis . They thought to benefit plant host by producing inhibitory compounds active against common fouling organisms. We investigated factors influence ability P. R. attach colonize also competitive interactions occur between these organisms other isolates from U. during biofilm formation on surface. A surprisingly high...
ABSTRACT The newly described green-pigmented bacterium Pseudoalteromonas tunicata (D2) produces target-specific inhibitory compounds against bacteria, algae, fungi, and invertebrate larvae is frequently found in association with living surfaces the marine environment. As part of our studies on ecology P. its interaction surfaces, we examined ability to form biofilms under continuous culture conditions within laboratory. exhibited a characteristic architecture consisting differentiated...
The use of wetlands is a promising technology to treat acid mine drainage, yet there little understanding the fundamental biological processes involved. They are considered centre on complex anaerobic ecology within sediments and involve removal metals by sulphate-reducing bacteria (SRB). These generate hydrogen sulphide cause precipitation from solution as insoluble metal sulphide. Sulphate-reducing have been isolated natural constructed receiving drainage. Sulphide production isolates...