A5037367467- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Nail Diseases and Treatments
- Essential Oils and Antimicrobial Activity
- Plant Pathogens and Fungal Diseases
- Bee Products Chemical Analysis
- Bacterial biofilms and quorum sensing
- Oral microbiology and periodontitis research
- Medical and Biological Ozone Research
- Urinary Tract Infections Management
- Phytochemistry Medicinal Plant Applications
- Fungal Biology and Applications
- Biological and pharmacological studies of plants
- Reproductive tract infections research
- Plant and animal studies
- Healthcare Regulation
- Nanoparticles: synthesis and applications
- Probiotics and Fermented Foods
- HIV/AIDS oral health manifestations
- Antimicrobial agents and applications
- Dental materials and restorations
- Insect and Pesticide Research
- Peptidase Inhibition and Analysis
- Cystic Fibrosis Research Advances
- Enterobacteriaceae and Cronobacter Research
Universidade Estadual de Maringá
2016-2025
Universidade Estadual do Paraná
2021
University of Minho
2009-2015
Institute for Biotechnology and Bioengineering
2009-2013
Universidade de São Paulo
2005-2007
The aim of this study was to evaluate the effect silver nanoparticles (SN) against Candida albicans and glabrata adhered cells biofilms. SN (average diameter 5 nm) were synthesized by nitrate reduction with sodium citrate stabilized ammonia. Minimal inhibitory concentration (MIC) tests performed for C. (n = 2) grown in suspension following Clinical Laboratory Standards Institute microbroth dilution method. applied (2 h) or biofilms (48 after 24 h contact their assessed enumeration colony...
Journal Article Silver nanoparticles: influence of stabilizing agent and diameter on antifungal activity against Candida albicans glabrata biofilms Get access D.R. Monteiro, Monteiro Department Dental Materials Prosthodontics, Araçatuba School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil Biological Engineering, Institute for Biotechnology Bioengineering, University Minho, Braga, Portugal Search other works by this author on: Oxford Academic Google Scholar S. Silva,...
The aim of this study was to compare biofilm formation by Candida glabrata and albicans on acrylic, either individually or when combined (single dual species) then examine the antimicrobial effects silver nanoparticles nystatin these biofilms. Candidal adhesion assays were performed acrylic surface in presence artificial saliva (AS) for 2 h 48 h, respectively. C. adherence determined number colony forming units (CFUs) recovered from biofilms CHROMagar(®) Candida. In addition, crystal violet...
Summary Although silver nanoparticles ( SN ) have been investigated as an alternative to conventional antifungal drugs in the control of C andida ‐associated denture stomatitis, activity combination with against biofilms remains unknown. Therefore, aim this study was evaluate efficacy nystatin NYT or chlorhexidine digluconate CHG albicans and glabrata biofilms. The alone combined were applied on mature (48 h), after 24 h treatment their antibiofilm activities assessed by total biomass...
Vulvovaginal candidiasis (VVC) is one of the most common genital infections in women. The therapeutic arsenal remains restricted, and some alternatives to VVC treatment are being studied. present study evaluated influence a propolis extractive solution (PES) on biofilm production by Candida albicans isolated from patients with VVC. Susceptibility testing was used verify minimum inhibitory concentration (MIC) PES, fluconazole nystatin as controls. formation 29 vaginal isolates C. reference...
Urinary tract infections (UTIs) are the most common nosocomial and 80 % related to use of urinary catheters. Furthermore, Candida species responsible for around 15 UTIs an increasing involvement non-Candida albicans (NCAC) (e.g. glabrata, tropicalis parapsilosis) has been recognized. Given fact that silicone is frequently used in manufacture catheters, aim this work was compare both adhesion biofilm formation on different clinical isolates NCAC (i.e. C. presence urine. Several recovered from...
Aim The aim of this study was to assess the effect different silver nanoparticles (SN) concentrations on matrix composition and structure Candida albicans glabrata biofilms. Methods Results biofilms were developed in 6-well microtiter plates during 48 h. After, these exposed 13·5 or 54 μg SN ml−1 for 24 Then, extracellular matrices extracted from analysed chemically terms proteins, carbohydrates DNA. To investigate biofilm structure, scanning electron microscopy (SEM) epifluorescence used....
Onychomycosis is a chronic fungal infection of nails, commonly caused by dermatophyte fungi, primarily species Trichophyton. Because the limited drug arsenal available to treat general infections and frequent failure onychomycosis treatment, search for new therapeutic sources essential, topical treatment with natural products has been encouraged. Propolis, an adhesive resinous compound produced honeybees (Apis mellifera), shown multiple biological properties including significant antifungal...
Aim: To evaluate the effect of propolis against Candida species planktonic cells and its counterpart's biofilms. Materials & methods: The MIC values, time-kill curves filamentation form inhibition were determined in cells. on biofilms was assessed through quantification CFUs. Results: ranging from 220 to 880 µg/ml, demonstrated higher efficiency C. albicans parapsilosis than tropicalis In addition, able prevent biofilm's formation eradicate their mature biofilms, coupled with a significant...
The present study evaluated the antibacterial and antibiofilm activity of carvacrol against Salmonella Typhimurium. minimum inhibitory concentration (MIC) bactericidal (MBC) were determined time-kill curve scanning electron microscopy (SEM) performed to evaluate activity. Antibiofilm was by quantifying total biomass using crystal violet assay, metabolic MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. action preformed biofilm on polypropylene stainless steel also...
The use of natural products such as propolis extract (PE) is a promising alternative when topically administered to replace conventional antifungals, mostly due its therapeutic applications, ease access and low toxicity. However, despite being the subject several mycology studies, they focus primarily on exploiting their antimicrobial activity, lacking information mechanisms action PE Candida spp., characterizing antifungal potential. To elucidate bioactivity cellular structure albicans. A...
Graphical abstract Biosynthesis and antifungal activity of silver nanoparticles stabilized with naringenin.
In vitro studies of adhesion capability are essential to characterise the virulence Candida species. However, assessment by traditional methods is time-consuming. The aim present study development a simple methodology using crystal violet staining quantify in different species epithelial cells. experiments performed albicans (ATCC 90028), C. glabrata 2001), parapsilosis 22019) and tropicalis 750). A human urinary bladder cell line (TCC-SUP) used. Yeast cells were stained with violet, then...
The present study evaluated the capacity of three species Fusarium isolated from onychomycosis to form biofilms and antibiofilm effect propolis extract on these biofilms.The effects were by quantifying colony-forming units, mitochondrial metabolic activity assays, total biomass crystal violet staining scanning electron microscopy.Propolis demonstrated significant efficiency spp. isolates reduced F. solani, oxysporum subglutinans mature biofilms.Propolis can be an alternative topical treatment caused
Aim: Elucidate the antifungal efficacy of biologically synthesized silver nanoparticles with ethanolic propolis extract (AgNPs PE) against planktonic forms and biofilms clinically important fungi. Materials & methods: AgNPs were synthesized, characterized evaluated for cytotoxicity, mutagenicity antimicrobial activity. Results: PE displayed a colloidal appearance, good stability size 2.0–40.0 nm. demonstrated lower cytotoxicity nonmutagenic potential. In addition, properties all tested...