A5031324285- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA Research and Splicing
- Malaria Research and Control
- Histone Deacetylase Inhibitors Research
- Neuroscience and Neuropharmacology Research
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Genetics and Neurodevelopmental Disorders
- Tissue Engineering and Regenerative Medicine
- vaccines and immunoinformatics approaches
- Neurogenesis and neuroplasticity mechanisms
- Antimicrobial Peptides and Activities
- Mexican Socioeconomic and Environmental Dynamics
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- Memory and Neural Mechanisms
- Alzheimer's disease research and treatments
- Advanced Proteomics Techniques and Applications
- Birth, Development, and Health
- Water Resource Management and Quality
- Machine Learning in Bioinformatics
- Metabolism and Genetic Disorders
- Invertebrate Immune Response Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
University of Pennsylvania
2017-2024
California State University Los Angeles
2020
Pontifical Catholic University of Peru
2009
Histone acetylation is governed by nuclear acetyl-CoA pools generated, in part, from local acetate metabolic enzyme synthetase 2 (ACSS2). We hypothesize that during gene activation, a transfer of intact occurs via sequential action epigenetic and enzymes. Using stable isotope labeling, we detect between histone sites both vitro using purified mammalian enzymes vivo quiescence exit Saccharomyces cerevisiae as change-of-state model. show Acs2, the yeast ortholog ACSS2, recruited to chromatin...
Histone acetylation is a key component in the consolidation of long-term fear memories. fueled by acetyl-coenzyme A (acetyl-CoA), and recently, nuclear-localized metabolic enzymes that produce this metabolite have emerged as direct local regulators chromatin. In particular, acetyl-CoA synthetase 2 (ACSS2) mediates histone mouse hippocampus. However, whether ACSS2 regulates memory remains to be determined. Here, we show Acss2 knockout well tolerated mice, yet Acss2-null exhibits reduced...
Histone posttranslational modifications (PTMs) frequently co-occur on the same chromatin domains or even in molecule. It is now established that these “histone codes” are result of cross talk between enzymes catalyze multiple PTMs with univocal readout as compared isolation. Here, we performed a comprehensive identification and quantification histone codes malaria parasite, Plasmodium falciparum. We used advanced quantitative middle-down proteomics to identify combinations both...
We set out to determine whether the C-terminus (amino acids 481-798) of peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α, UniProt Q9UBK2), a regulatory metabolic protein involved in mitochondrial biogenesis, and respiration, is an arginine methyltransferase substrate. Arginine methylation by methyltransferases (PRMTs) alters function thus contributes various cellular processes. In addition confirming PRMT1 as described literature, we have identified another member...
Protein translational control is critical for ensuring that the fetus develops correctly and necessary organs tissues are formed functional. We developed an in utero method to quantify tissue-specific protein dynamics by monitoring amino acid incorporation into proteome after pulse injection. Fetuses of pregnant mice were injected with isotopically labeled lysine arginine via vitelline vein at various embyonic days, harvested. By analyzing nascent proteome, unique signatures each tissue...
ABSTRACT Dysregulation of histone acetylation in the brain has emerged as a major contributor to human Alzheimer’s disease (AD). The mechanisms by which these protective or risk-conferring epigenetic marks are established and maintained under intense investigation. ACSS2 (Acetyl-CoA Synthetase 2) is key metabolic enzyme that chromatin-associated neurons. recruited specific promoters generates local pool acetyl-CoA from acetate, thereby fueling driving expression neuronal genes regulate...
ABSTRACT Histone acetylation is a key component in the consolidation of long-term fear memories. Epigenetic enzymes involved histone acetylation, including acetyltransferases and deacetylases, have been put forward as potential pharmacological targets treatment pathological memories, such those that underlie post-traumatic stress disorder (PTSD). However, these typically play ubiquitous role gene regulation, which precludes clinical use systemic manipulations. Recently, we found...
Abstract Protein translational control is highly regulated step in the gene expression program during mammalian development that critical for ensuring fetus develops correctly and all of necessary organs tissues are formed functional. Defects protein fetal can lead to severe developmental abnormalities or premature death. Currently, quantitative techniques monitor synthesis rates a developing ( utero ) limited. Here, we developed novel stable isotope labeling approach quantify...
Actualmente el Estado y la sociedad civil promueven campañas públicas destinadas a crear una cultura de ahorro del agua en ciudadanía. Se apela concientización sobre finitud recurso se busca que las personas cambien prácticas derrochadoras por responsables con agua. Sin embargo, promedio casi mitad producen empresas saneamiento pierde fugas tuberías matrices; falta medidores promueve desperdicio los ciudadanos. La carencia voluntad política impopularidad generaría incorporación medidas...
We set out to investigate the effects of two PTMs, methylation and phosphorylation, with goal understanding role arginine on neighboring serine phosphorylation in histone H3. Work by other labs has confirmed that are involved crosstalk within an AKT motif (RXRXXS/T) where residue is residues away from residue. Herein, we use N‐terminal tail H3 focus its arginine‐lysine‐serine (RKS) determine whether a similar interaction taking place. Serine‐10 hyper known biomarker for tumorigenesis,...
Abstract A ‘histone code’ defines system-level crosstalk between histone post-translational modifications (PTMs) to induce specific biological outcomes. Proteome-scale information of co-existing PTM across the entire chromatin landscape malaria parasite, Plasmodium falciparum, was lacking. Here, we used advanced quantitative middle-down proteomics identify combinations PTMs in both proliferative, asexual stages and transmissible, sexual gametocyte P. falciparum . We provide an updated,...