A5087400641- Gut microbiota and health
- Cancer Cells and Metastasis
- Digestive system and related health
- T-cell and B-cell Immunology
- Gastrointestinal motility and disorders
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Probiotics and Fermented Foods
- Inflammatory Bowel Disease
- Circadian rhythm and melatonin
- Immune cells in cancer
- Effects of Radiation Exposure
- Breast Cancer Treatment Studies
- Viral gastroenteritis research and epidemiology
- Gastrointestinal disorders and treatments
- Genomics and Chromatin Dynamics
- Antibiotic Resistance in Bacteria
- Microfluidic and Bio-sensing Technologies
- Celiac Disease Research and Management
- FOXO transcription factor regulation
- Pregnancy and Medication Impact
- Escherichia coli research studies
- Vehicle Routing Optimization Methods
- Bacterial Genetics and Biotechnology
- Estrogen and related hormone effects
Scripps Research Institute
2017-2021
Washington University in St. Louis
2010-2019
Janssen (United States)
2019
Ewha Womans University
2017
Howard Hughes Medical Institute
2010
Pulmonary and Critical Care Associates
2010
Although CD103-expressing dendritic cells (DCs) are widely present in nonlymphoid tissues, the transcription factors controlling their development and relationship to other DC subsets remain unclear. Mice lacking factor Batf3 have a defect of CD8α+ conventional DCs (cDCs) within lymphoid tissues. We demonstrate that Batf3−/− mice also lack CD103+CD11b− lung, intestine, mesenteric lymph nodes (MLNs), dermis, skin-draining nodes. Notably, displayed reduced priming CD8 T after pulmonary Sendai...
<h3>Objective</h3> The technology for the growth of human intestinal epithelial cells is rapidly progressing. An exciting possibility that this system could serve as a platform individualised medicine and research. However, to achieve goal, culture must be enhanced so biopsies from individuals can used reproducibly generate cell lines in short time frame multiple, functional assays performed (ie, barrier function host–microbial interactions). <h3>Design</h3> We created large panel...
<h3>Background</h3> The small intestinal epithelium is highly sensitive to radiation and a major site of injury during therapy environmental overexposure. <h3>Objective</h3> To examine probiotic bacteria as potential radioprotective agents in the intestine. <h3>Methods</h3> 8-week-old C57BL/6 wild-type or knockout mice were administered by gavage for 3 days before 12 Gy whole body radiation. intestine was evaluated cell-positional apoptosis (6 h) crypt survival (84 h). <h3>Results</h3>...
Tandem mass spectrometry based shotgun proteomics of distal gut microbiomes is exceedingly difficult due to the inherent complexity and taxonomic diversity samples. We introduce two new methodologies improve metaproteomic studies microbiome These methods include stable isotope labeling in mammals permit protein quantitation across mouse cohorts as well application activity-based probes enrich analyze both host microbial proteins with specific functionalities. used these technologies study...
Metabolites that are dysregulated in a mouse model of inflammatory bowel disease identified and characterized.
Cysteine proteases are among the most abundant hydrolytic enzymes produced by bacteria, and this diverse family of proteins have significant biological roles in bacterial viability environmental interactions. Members clostripain-like (C11) cysteine from commensal gut strains recently been shown to mediate immune responses inducing neutrophil phagocytosis activating pathogenic toxins. Development substrates, inhibitors, probes that target C11 enteric bacteria will help establish role these at...
Metaproteomics can greatly assist established high-throughput sequencing methodologies to provide systems biological insights into the alterations of microbial protein functionalities correlated with disease-associated dysbiosis intestinal microbiota. Here, authors utilize well-characterized murine T cell transfer model colitis find specific changes within luminal proteome associated inflammation. MS proteomic analysis colonic samples permitted identification ≈10 000-12 000 unique peptides...
Non-productive antigen receptor genes with frame shifts generated during the assembly of these are found in many mature lymphocytes. Transcripts from have premature termination codons (PTCs) and could encode truncated proteins if they not either inactivated or destroyed by nonsense-mediated decay (NMD). In mammalian cells, NMD can be activated pathways that rely on presence an intron downstream PTC; however, also do introns, independent inactivate PTC-containing transcripts. Here, through...
Abstract Metaproteomics can greatly assist established high-throughput sequencing methodologies to provide systems biological insights into the alterations of microbial protein functionalities correlated with disease-associated dysbiosis intestinal microbiota. Here, we utilized well-characterized murine T cell transfer model colitis find specific changes within luminal proteome associated inflammation. MS proteomic analysis colonic samples permitted identification ∽10,000-12,000 unique...
Abstract Human intestinal organoids have enabled performance of functional epithelial studies and modeling human diseases the intestine. This unit describes 1) a method to isolate culture crypts from tissue, 2) use combinatorial methods expand stem cell–enriched spheroids differentiate them into composed various cell types, 3) stimulate these with measure their responsiveness external stimuli. To validate differentiation, can be stained qualitatively evaluate presence colonic crypt...
Background: Tonsil-derived mesenchymal stem cells (TMSCs), prepared after tonsillectomy in human, have many advantages including a short doubling time, high differentiation capacity, and mixed chimerism property. Metformin has an anti-inflammatory function involving inhibition of signal transducer activator transcription (STAT)-3. We aimed to evaluate the effect metformin-pretreated TMSCs mouse model chronic colitis. Methods: The optimal condition under which maximum decrease STAT-3 level...
Abstract Background: It is estimated that approximately 46,000 women age &gt;75 are diagnosed annually with breast cancer. Due to competing co-morbidities, there wide variation in treatment recommendations which can lead over- or under-treatment. Though surgery for cancer considered low-morbidity, many elderly given a choice, choose not have surgery. Previous randomized trials comparing tamoxifen versus endocrine therapy alone &gt;70 unselected ER status demonstrated similar overall...