A5067222665- Plant Reproductive Biology
- Plant Molecular Biology Research
- Plant and animal studies
- Chromosomal and Genetic Variations
- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Plant Virus Research Studies
- Advances in Cucurbitaceae Research
- Plant Physiology and Cultivation Studies
- Genetic Mapping and Diversity in Plants and Animals
- Plant biochemistry and biosynthesis
- Glycosylation and Glycoproteins Research
- Plant pathogens and resistance mechanisms
- Plant Genetic and Mutation Studies
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Ubiquitin and proteasome pathways
- Bioenergy crop production and management
- Genomics and Phylogenetic Studies
- Botanical Research and Chemistry
- Polysaccharides and Plant Cell Walls
- Plant Taxonomy and Phylogenetics
- Horticultural and Viticultural Research
- Peptidase Inhibition and Analysis
- Lichen and fungal ecology
Chiba University
2013-2024
Yokohama City University
1997-2005
Kihara Institute for Biological Research
1997-2005
Kyoto University
1999-2003
Yamagata University
1999
Nagoya University
1999
Institute of Agrobiological Sciences
1997
Gametophytic self-incompatibility in Rosaceae, Solanaceae, and Scrophulariaceae is controlled by the S locus, which consists of an S-RNase gene unidentified "pollen S" gene. An ∼70-kb segment locus rosaceous species almond, haplotype–specific region containing gene, was sequenced completely. This found to contain two pollen-expressed F-box genes that are likely candidates for pollen genes. One them, named SFB (S protein), expressed specifically showed a high level sequence polymorphism,...
This report identifies S -RNases of sweet cherry ( Prunus avium L.) and presents information about cDNA sequences encoding the -RNases, which leads to development a molecular typing system for -alleles in this fruit tree species. Stylar proteins were surveyed by two dimensional polyaclylamide gel electrophoresis (2D-PAGE) identify -proteins associated with gametophytic self-incompatibility. Glycoprotein spots linked found group had Mr pI similar those other rosaceous -RNases. These...
Abstract Low glutelin content1 (Lgc1) is a dominant mutation that reduces content in rice grains. Glutelin major seed storage protein encoded by multigene family. RNA gel blot and reverse transcriptase–mediated PCR analyses revealed Lgc1 acts at the mRNA level similarity-dependent manner. In homozygotes, there 3.5-kb deletion between two highly similar genes forms tail-to-tail inverted repeat, which might produce double-stranded molecule, potent inducer of silencing. The hypothesis...
This study describes a novel F-box protein gene in the S-locus of sour cherry (Prunus cerasus) and sweet (P. avium). The showed an S-haplotype-specific sequence polymorphism expression was specific to pollen. Genomic DNA blot analysis eight cultivars with probe for under low stringency conditions yielded RFLP bands S-haplotypes each cultivar. We discuss possibility being candidate male determinant gametophytic self-incompatibility Prunus.
Self-Incompatibility-Related RNases in Styles of Japanese Pear (Pyrus serotina Rehd.) Get access Hidenori Sassa, Sassa 1Plant Breeding Laboratory, Department Horticulture, Chiba UniversityMatsudo 648, Matsudo City, Chiba, 271 Japan Search for other works by this author on: Oxford Academic Google Scholar Hisashi Hirano, Hirano 2Growth-Regulating Gene Molecular Biology, National Institute Agrobiological ResourcesKannondai 2-1-2, Tsukuba Ibaraki, 305 Hiroshi Ikehashi Plant and Cell Physiology,...
Abstract Although recent findings suggest that the F-box genes SFB/SLF control pollen-part S specificity in S-RNase-based gametophytic self-incompatibility (GSI) system, how these operate system is unknown, and functional variation of pollen different species has been reported. Here, we analyzed locus two Maloideae: apple (Malus domestica) Japanese pear (Pyrus pyrifolia). The sequencing a 317-kb region S9 haplotype revealed similar genes. Homologous sequences were isolated from haplotypes...
Grain size is a major yield component in rice, and partly controlled by the sizes of lemma palea. Molecular mechanisms controlling these organs largely remain unknown. In this study, we show that an antagonistic pair basic helix-loop-helix (bHLH) proteins involved determining rice grain length cell lemma/palea. Overexpression atypical bHLH, named POSITIVE REGULATOR OF GRAIN LENGTH 1 (PGL1), lemma/palea increased weight transgenic rice. PGL1 non-DNA-binding bHLH assumed to function as...
Stylar proteins of 13 almond (Prunus dulcis) cultivars with known S-genotypes were surveyed by IEF and 2D-PAGE combined immunoblot N-terminal amino acid sequence analyses to identify S-RNases associated gametophytic self-incompatibility (SI) in this plant species. RNase activities corresponding Sa Sb, two the four S-alleles tested, identified activity staining. The Sa-RNase band reacted anti-S4serum prepared from Japanese pear (Pyrus serotina); no reaction antiserum was observed sbRNase...
Summary Monofactorial gametophytic self‐incompatibility in the family Rosaceae is controlled by single multi‐allelic S ‐locus. In Japanese pear, a naturally occurring self‐compatible mutant was found as sport of self‐incompatible cultivar with an ‐genotype 2,S 4 . The S,4 allele has been shown to be non‐functional style mutant, but functional pollen. present study shows 2 ‐RNase gene transcribed normally that transcript not detectable. Furthermore, could detected genomic Southern blot,...
Grain size is an important yield component in rice, however, genes controlling the trait remain poorly understood. Previously, we have shown that antagonistic pair of basic helix-loop-helix (bHLH) proteins, POSITIVE REGULATOR OF GRAIN LENGTH 1 (PGL1) and ANTAGONIST PGL1 (APG), involved rice grain length. Here, report involvement another atypical bHLH protein gene, 2 (PGL2), regulation Over-expression PGL2 lemma/palea increased length weight correlation with level transgene expression....
The S-RNase-based gametophytic self-incompatibility (SI) of Rosaceae, Solanaceae, and Plantaginaceae is controlled by at least two tightly linked genes located the complex S locus; highly polymorphic S-RNase for pistil specificity F-box gene (SFB/SLF) pollen. Self-incompatibility in Prunus (Rosaceae) considered to represent a 'self recognition single factor' system, because loss-of-function SFB associated with self-compatibility, allelic divergence high comparable that S-RNase. In contrast,...
Summary Barley ( H ordeum vulgare ) spikes are developmentally switched from two‐rowed to six‐rowed by a single recessive gene, spike 1 vrs1 ), which encodes homeodomain‐leucine zipper I class transcription factor. Vrs1 is paralog of HvHox2 and both were generated duplication an ancestral gene. conserved among cereals, whereas acquired its current function during the evolution barley. It was unclear whether divergence expression pattern or protein accounted for functionalization . Here, we...
<i>N</i> <sup>α</sup>-Acetylation, catalyzed co-translationally with <sup>α</sup>-acetyltransferase (NAT), is the most common modifications of eukaryotic proteins. In yeast, there are at least three NATs: NAT1, MAK3, and NAT3. The 20 S proteasome subunits were purified from normal strain each deletion mutants,<i>nat1, mak3</i>, <i>nat3</i>. electrophoretic mobility these was compared by two-dimensional gel electrophoresis. Shifts toward alkaline side unblocking N terminus certain in one or...
Abstract Almond has a self-incompatibility system that is controlled by an S locus consisting of the S-RNase gene and unidentified “pollen gene.” An almond cultivar “Jeffries,” somaclonal mutant “Nonpareil” (ScSd), dysfunctional Sc haplotype both in pistil pollen. Immunoblot genomic Southern blot analyses detected no haplotype-specific signal Jeffries. showed Jeffries extra copy Sd haplotype. These results indicate at least two mutations had occurred to generate Jeffries: (1) deletion (2)...
Gametophytic self-incompatibility (GSI) in Solanaceae, Rosaceae, and Plantaginaceae is controlled by a multiallelic S-locus. The specificities of pistil pollen are separate S-locus genes, S-RNase SLF/SFB, respectively. Although the S-specificity determined factors located outside also required for expression GSI. HT-B one non-S-factors identified Nicotiana Solanum, encodes small asparagine/aspartate-rich extracellular protein with unknown biochemical function. Here, was cloned from Petunia...