A5101818876- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- RNA regulation and disease
- Microbial Metabolic Engineering and Bioproduction
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Advanced Photocatalysis Techniques
- Cancer Research and Treatments
- Enzyme Catalysis and Immobilization
- Mosquito-borne diseases and control
- Plant-Microbe Interactions and Immunity
- Pituitary Gland Disorders and Treatments
- Nanoplatforms for cancer theranostics
- Plant Pathogens and Fungal Diseases
- Pluripotent Stem Cells Research
- CAR-T cell therapy research
- Plant Pathogenic Bacteria Studies
- Gene Regulatory Network Analysis
- Endoplasmic Reticulum Stress and Disease
- Biofuel production and bioconversion
- Nanoparticle-Based Drug Delivery
- Genetics, Aging, and Longevity in Model Organisms
- 14-3-3 protein interactions
- Sarcoidosis and Beryllium Toxicity Research
Inner Mongolia Agricultural University
2024-2025
AstraZeneca (Sweden)
2018-2024
Novo Nordisk Foundation
2016-2024
Technical University of Denmark
2016-2024
Nanchang Hangkong University
2022-2024
Shanghai Jiao Tong University
2023
Shanghai Sixth People's Hospital
2023
Chonnam National University Hwasun Hospital
2012-2018
Fuzhou General Hospital of Nanjing Military Command
2017
Foundation Center
2016
Abstract Genome editing, specifically CRISPR/Cas9 technology, has revolutionized biomedical research and offers potential cures for genetic diseases. Despite rapid progress, low efficiency of targeted DNA integration generation unintended mutations represent major limitations genome editing applications caused by the interplay with double-strand break repair pathways. To address this, we conduct a large-scale compound library screen to identify targets enhancing insertions. Our study reveals...
Abstract Prime editing recently emerged as a next-generation approach for precise genome editing. Here we exploit DNA double-strand break (DSB) repair to develop two strategies that install genomic insertions using an Sp Cas9 nuclease-based prime editor (PEn). We first demonstrate PEn coupled regular guide RNA (pegRNA) efficiently promotes short through homology-dependent DSB mechanism. While leads increased levels of by-products, it can rescue pegRNAs perform poorly with nickase-based...
Base Editing is a precise genome editing method that uses deaminase-Cas9 fusion protein to mutate cytidine thymidine in target DNA situ without the generation of double-strand break. However, efficient enrichment genetically modified cells using this technique limited by ability detect such events.We have developed FLuorescent Activity REporter (BE-FLARE), which allows for undergone loci based on fluorescence shift from BFP GFP. We used BE-FLARE evaluate efficiency APOBEC3A and APOBEC3B...
Growth decoupling can be used to optimize microbial production of biobased compounds by inhibiting excess biomass formation and redirect carbon flux from growth product formation. However, identifying suitable genetic targets through rational design is challenging. Here, we conduct a genome-wide CRISPRi screen discover switches for production. Using an sgRNA library covering 12 238 loci in the Escherichia coli genome, that inhibit while allowing continued protein In total, identify 1332...
Prokaryotic restriction enzymes, recombinases and Cas proteins are powerful DNA engineering genome editing tools. However, in many primary cell types, the efficiency of remains low, impeding development gene- cell-based therapeutic applications. A safe strategy for robust efficient enrichment precisely genetically engineered cells is urgently required. Here, we screen mutations receptor Diphtheria Toxin (DT) which protect human from DT. Selection with an edited DT variant enriches...
Myxobacteria have a complex life cycle and unique social behavior, obtain nutrients by preying on bacteria fungi in soil. Chitinase, β-1,3 glucanase β-1,6 produced myxobacteria can degrade the glycosidic bond of cell wall some plant pathogenic fungi, resulting perforated structure wall. In addition, isooctanol lead to accumulation intracellular reactive oxygen species induce apoptosis. also perforate oomycetes glucanase, reduce content soluble protein protective enzyme activity, affect...
Glioblastoma is a highly lethal neoplasm that frequently recurs locally after radiotherapy, and most of these recurrences originate from near the irradiated target field. In present study, we identified effects radiation on glioma invasion p53, TIMP-2, MMP-2 expression through in vitro vivo experiments. The U87MG (wt p53) U251 (mt human malignant cell lines were prepared, U2OS 53) Saos2 (del osteosarcoma used as p53 positive negative controls. four knock-downed cells received (2–6 Gy)...
Chinese hamster ovary (CHO) cells are the preferred workhorse for biopharmaceutical industry, and CRISPR/Cas9 has proven powerful generating targeted gene perturbations in CHO cells. Here, we expand CRISPR engineering toolbox with activation (CRISPRa) to increase transcription of endogenous genes. We successfully increased Mgat3 St6gal1, verified their activity on a functional level by subsequently detecting that appropriate glycan structures were produced. This study demonstrates CRISPRa...
Pooled CRISPR screens have been widely applied to mammalian and other organisms elucidate the interplay between genes phenotypes of interest. The most popular method for delivering components into cells is lentivirus based. However, because not always an option, virus-free protocols are starting emerge. Here, we demonstrate improved virus-free, genome-wide screening platform Chinese hamster ovary with 75,488 gRNAs targeting 15,028 genes. Each gRNA expression cassette in library precisely...
Streptococcus pyogenes Cas9 (SpCas9) and derived enzymes are widely used as genome editors, but their promiscuous nuclease activity often induces undesired mutations chromosomal rearrangements. Several strategies for mapping off-target effects have emerged, they suffer from limited sensitivity. To increase the detection sensitivity, we develop an assessment workflow that uses Duplex Sequencing. The strategy increases sensitivity by one order of magnitude, identifying previously unknown...
Myxobacteria are a special kind of Gram-negative bacteria that can slide and produce variety bioactive substances against bacteria, fungi, viruses. It has great development research value in medicine agriculture. Although myxobacteria have become hotspot at home abroad, there few systematic studies on the relationship between its diversity, geographical location, environment factors. In order to solve these problems, 133 soil samples were collected from east west Inner Mongolia Autonomous...
Protein disulfide isomerase (PDI) acts as a chaperone on the cell surface, and it has been reported that PDI is associated with tumor migration invasion. The aims of this study are to investigate anti-migration effect bacitracin, which an inhibitor PDI, factor in process.U87-MG glioma cells were treated bacitracin 1.25, 2.5, 3.75, 5.0 mM concentrations. Western blot caspase-3 was applied evaluate cytotoxicity bacitracin. Adhesion, morphology, assays, organotypic brain-slice culture performed...
Induced pluripotent stem cell (iPSC) derived endothelial cells (iECs) have emerged as a promising tool for studying vascular biology and providing platform modelling various diseases, including those with genetic origins. Currently, primary ECs are the main source disease in this field. However, they difficult to edit limited lifespan. To study effects of targeted mutations on an endogenous level, we generated characterized iPSC model venous malformations (VMs). CRISPR-Cas9 technology was...
OBJECTIVE In patients with glioblastoma, local invasion of tumor cells causes recurrence and shortens survival. The goal this study was to determine whether protein disulfide isomerase (PDI) A6 regulates migration glioblastoma the associated factors. METHODS U87MG were treated either PDIA6 or ADAM17 small interfering RNA (siRNA) fragments both types siRNA fragments, expression confirmed by reverse transcription–polymerase chain reaction Western blot. Migration assessed using a wound-healing...
Interference with genes is the foundation of reverse genetics and key to manipulation living cells for biomedical biotechnological applications. However, classical genetic knockout transcriptional knockdown technologies have different drawbacks offer no control over existing protein levels. Here, we describe an efficient genome editing approach that affects specific abundances by changing rates both RNA synthesis degradation, based on two cross-kingdom mechanisms CRISPRi N-end rule...