A5008864692- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- vaccines and immunoinformatics approaches
- Quantum Dots Synthesis And Properties
- Insect Resistance and Genetics
- Phytochemical compounds biological activities
- Viral gastroenteritis research and epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Advanced Nanomaterials in Catalysis
- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- Invertebrate Immune Response Mechanisms
- Tuberculosis Research and Epidemiology
- Viral Infections and Immunology Research
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Studies on Chitinases and Chitosanases
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Plant-Derived Bioactive Compounds
Shizuoka University
2018-2024
National Research and Innovation Agency
2023
Information and Communication Technology Agency
2010
Pharmac
2010
Dengue surveillance trusts only on reverse transcription-polymerase chain reaction (RT-PCR) type methodologies for confirmation of dengue virus serotypes; however, its real time application is restricted due to the expensive, complicated, and time-consuming process. In search a new sensing system, here, we have reported two-way-detection method (DENV) serotype identification along with DNA quantification by using class nanocomposite gold nanoparticles (AuNP) nitrogen, sulfur codoped graphene...
The treatment for mosquito-borne viral diseases such as dengue virus (DENV), zika (ZIKV), and chikungunya (CHIKV) has become difficult due to delayed diagnosis processes. In addition, sharing the same transmission media similar symptoms at early stage of infection these more critical diagnosis. To overcome this, a common platform that can identify with high sensitivity selectivity, even different serotypes, is in demand. this study, we have attempted an electrochemical impedimetric method...
To develop monovalent dengue virus-like particle for serotype 3 (DENV-LP/3), we prepared and expressed two structural polyprotein constructs using silkworm Bm5 cells: DENV-3 Capsid-premembrane-envelope (DENV-3CprME) premembrane-envelope (DENV-3prME). The PA-tagged 3CprME 3prME polypeptides were partially purified by PA-tag affinity chromatography had molecular weights of 85 75 kDa, respectively. Expressed proteins separately verified the following primary antibodies: anti-PA tag antibody,...
Dengue is an arboviral disease, which threatens almost half the global population, and has emerged as most significant of current public health challenges. In this study, we prepared dengue virus-like particles (DENV-LPs) consisting Capsid-premembrane-envelope (CprME) premembrane-envelope (prME) polypeptides from serotype 1 4, were expressed in silkworms using Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid. 1CprME, 1prME, 4CprME, 4prME proteins hemolymph, molecular weight purified was 55...
Foot-and-mouth disease (FMD) is a highly contagious that infects cloven-hoofed animals, becoming serious threat to livestock production and leading significant economic losses. The re-occurring FMD outbreak in Indonesia was reported back 2022, causing hundreds of cattle deaths. immunogenic viral capsid VP1 protein has been extensively researched as vaccine candidate despite the fact existing uses an inactivated virus. vp1 gene (648 bps) from virus serotype O integrated into pET-32b vector...
Mycobacterium tuberculosis (Mtb) is a pathogenic bacteria responsible for (TB), an infectious disease that poses significant threat in Indonesia. Despite extensive and thorough research efforts throughout the years, Bacille Calmette-Guerin (BCG) remains sole authorized vaccination with varying levels of effectiveness. It offers immunity against children but not efficacious treating adults. Epidemiological modelling indicates that, despite advancements pharmacological treatments tuberculosis,...
Rocaglamide derivatives are the compounds which have featuring cyclopenta[b]tetra-hydrobenzofuran skeleton. Until now it includes more than 50 naturally occurring derivatives. They were chosen to be interesting candidates for possible therapeutic agents primarily in field of cancer chemotherapy due their cytotoxic activities data against various cells. A quantitative structure activity relationship (QSAR) studies done investigate physicochemical properties molecule contribute activities....
NS3 protein is 618 amino acids (aa) in length containing serine protease and helicase domains required for DENV replication. Alignment of consensus acid sequences from all four serotypes demonstrated that this more conserved (78%) among the different dengue serotypes, which elicits a strong cellular immune response after viral infection humans animal models. Present study, central hydrophilic region cofactor, NS2B (NS2BH) with full genes DENV3 Indonesian strain were amplified CDNA following...