A5105003505- Renin-Angiotensin System Studies
- Receptor Mechanisms and Signaling
- Hormonal Regulation and Hypertension
- Nitric Oxide and Endothelin Effects
- Renal and related cancers
- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Erythropoietin and Anemia Treatment
- Apelin-related biomedical research
- Protein Kinase Regulation and GTPase Signaling
- Acute Kidney Injury Research
- Sodium Intake and Health
- Retinal Development and Disorders
- 3D Printing in Biomedical Research
- RNA and protein synthesis mechanisms
- Eicosanoids and Hypertension Pharmacology
- Nuclear Receptors and Signaling
- Cardiomyopathy and Myosin Studies
- Inflammatory mediators and NSAID effects
- Connexins and lens biology
- Insect Resistance and Genetics
- Blood transfusion and management
- Cardiac Fibrosis and Remodeling
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Renal and Vascular Pathologies
University of Regensburg
2007-2019
Czech Academy of Sciences, Institute of Physiology
2009
University of Zurich
1984-2005
Roche (United States)
1999
Inserm
1995
Collège de France
1995
Vanderbilt University
1993
John Radcliffe Hospital
1993
Thomas Jefferson University
1993
cGMP-based regulatory systems are vital for counteracting the renin-angiotensin system (RAS) which promotes volume expansion and high blood pressure. Natriuretic peptides nitric oxide acting through their second messenger cGMP normally increase natriuresis diuresis, regulate renin release; however, severe pathological state of cardiac heart failure is characterized by elevated levels atrial natriuretic peptide that no longer able to effectively oppose exaggerated RAS effects. There presently...
This study sought to examine the involvement of prostaglandins and nitric oxide (NO) in macula densa-dependent activation renin system vivo. For this purpose, male Sprague-Dawley rats were chronically infused with furosemide (12 mg/day) for 6 days inhibit densa salt transport. To synthesis NO, animals injected indomethacin (2 mg/kg twice daily) nitro-L-arginine methyl ester (L-NAME; 40 last 2 experiment, respectively. Furosemide infusion increased plasma activity (PRA) from 8.8 +/- 1.4 41...
Abstract This study aimed to characterize the influence of dietary salt intake on gene expression angiotensin II type 1 (AT ) receptor subtypes in different organs. Male Sprague-Dawley rats were fed low (0.2 mg/g), normal (6 or high (40 mg/g) diets for 5, 10, and 20 days. mRNA levels two AT determined adrenal gland, kidney, liver, lung. In all organs examined, with exception glands, diet led a transient decrease abundance 1A but not 1B mRNA, which reached their nadirs between days 5 10...
ABSTRACT Renin secretion at the level of renal juxtaglomerular cells appears to be controlled mainly by classic second messengers such as Ca2+, cyclic AMP and GMP, which in turn exert their effects through oppositely acting protein kinases probably also affecting activity ion channels plasma membrane. Thus, kinase A stimulates renin secretion, whilst C G II inhibit secretion. Moreover, Cl− could involved mediation inhibitory action Ca2+ on This review summarizes our present knowledge about...
In this study we have characterized the synthesis and secretion of renin in short-term cultures isolated mouse renal juxtaglomerular (JG) cells. Our findings suggest that those can be utilized to control parallel. With experimental model, examined whether are concordantly regulated by second-messenger molecules such as cyclic nucleotides protein kinase C. We found 8-bromo-cyclic GMP (8-bromo-cGMP; 100 µM) inhibited forskolin (1 isoproterenol (10 enhanced basal during a 20-hour incubation...
The activity of the renin-angiotensin-aldosterone system is triggered by release protease renin from kidneys, which in turn controlled sense negative feedback loops. It widely assumed that Ang II (angiotensin II) directly inhibits expression and secretion via a short-loop an effect on renin-producing cells (RPCs) mediated AT1 (Ang type 1) receptors. Because concept such direct control, originates mostly vitro experiments, has not yet been systematically proven vivo, we aimed to test validity...
The retina expresses a local renin-angiotensin system (RAS). This study aimed to investigate the influence of systemic modulation renin synthesis on expression in retinal pigment epithelium (RPE), which forms part blood/retina barrier. Freshly isolated RPE cells showed 1A, is secreted isoform renin. Systemic administration angiotensin-converting enzyme inhibitor enalapril mice increased both kidney and retina. infusion ANG II led decrease RPE. II-dependent down-regulation was prevented by...
The aim of this study was to determine the role renal innervation in prolonged stimulation renin secretion and synthesis accompanying artery stenosis. Male Sprague-Dawley rats, which left kidney had been denervated or sham 4 days earlier, received a clip (ID 0.2 mm). Plasma activity mRNA were assayed 1, 2, after clipping. both plasma blunted markedly rats with clipped kidney. typical suppression intact right kidney, however, not different between sham-denervated kidneys, nor increase blood...
During nephrogenesis, renin expression shifts from the vessel walls of interlobular arteries to terminal portions afferent arterioles in a wavelike pattern. Since mechanisms responsible for developmental deactivation are as yet unknown, we hypothesized that developing renin-angiotensin system (RAS) may downregulate itself via negative feedback prevent overactivity renin. To test possible role angiotensin II expression, studied development intrarenal mice lacking ANG AT1a, AT1b, or AT2...
The salt intake of an organism controls the number renin-producing cells in kidney by yet undefined mechanisms. This study aimed to assess a possible mediator role preglomerular blood pressure control renin expression oral intake. We used wild-type (WT) mice and lacking angiotensin II type 1a receptors (AT(1a)-/-) displaying enhanced sensitivity expression. In WT kidneys, we found renin-expressing at ends all afferent arterioles. A low-salt diet (0.02%) led moderate twofold increase along...