A5082641442- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Natural product bioactivities and synthesis
- Microbial Natural Products and Biosynthesis
- Sesquiterpenes and Asteraceae Studies
- Antioxidant Activity and Oxidative Stress
- Biological Activity of Diterpenoids and Biflavonoids
- Sirtuins and Resveratrol in Medicine
- Essential Oils and Antimicrobial Activity
- GABA and Rice Research
- Coconut Research and Applications
- Traditional and Medicinal Uses of Annonaceae
- Photosynthetic Processes and Mechanisms
Institute for Integrative Systems Biology
2024
Center for Research in Agricultural Genomics
2014-2018
Universitat de Barcelona
2000-2017
Abstract Rosemary extracts containing the phenolic diterpenes carnosic acid and its derivative carnosol are approved food additives used in an increasingly wide range of products to enhance shelf-life, thanks their high anti-oxidant activity. We describe here elucidation complete biosynthetic pathway reconstitution yeast cells. Cytochrome P450 oxygenases (CYP76AH22-24) from Rosmarinus officinalis Salvia fruticosa already characterized as ferruginol synthases also able produce...
Carnosic acid (CA) is a phenolic diterpene with anti-tumour, anti-diabetic, antibacterial and neuroprotective properties that produced by number of species from several genera the Lamiaceae family, including Salvia fruticosa (Cretan sage) Rosmarinus officinalis (Rosemary). To elucidate CA biosynthesis, glandular trichome transcriptome data S. were mined for terpene synthase genes. Two putative genes, namely SfCPS SfKSL, showing similarities to copalyl diphosphate kaurene synthase-like...
The enzyme dehydrodolichyl diphosphate (dedol‐PP) synthase is a cis ‐prenyltransferase that catalyzes the synthesis of dedol‐PP, long‐chain polyprenyl used as precursor for dolichyl phosphate. Here we report cloning and characterization cDNA from Arabidopsis thaliana encoding dedol‐PP synthase. identity cloned was confirmed by functional complementation yeast mutant strain defective in activity together with detection high levels transformed mutant. A. mRNA detected at roots but hardly...
Guaianolides are an important class of sesquiterpene lactones with unique biological and pharmaceutical properties. They have been postulated to be derived from germacranolides, but for years no progress has made in the elucidation their biosynthesis that requires unknown cyclization mechanism. Here we demonstrate isolation characterization a cytochrome P450 feverfew (Tanacetum parthenium), kauniolide synthase. Kauniolide synthase catalyses formation guaianolide germacranolide substrate...
Abstract Mulberry ( Morus alba L.) is considered a millenary medicinal plant and food source for silkworms. Different M. extracts offer variety of biological pharmacological properties that are in part attributed to stilbenoids, small group phenylpropanoids include resveratrol oxyresveratrol. These naturally present non-renewable parts mulberry trees, impeding their efficient extraction. As way bypass this spatiotemporal restriction, we generated cell suspensions from twigs demonstrated the...
Isoprenoids comprise the largest class of natural compounds and are found in all kinds organisms. In plants, they participate both primary specialized metabolism, playing essential roles nearly aspects growth development. The enormous diversity this family is extensively exploited for biotechnological biomedical applications as biomaterials, biofuels or drugs. Despite their variety structures, isoprenoids derive from common C5 precursor isopentenyl diphosphate (IPP). Plants synthesize IPP...