A5032598793- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Anaerobic Digestion and Biogas Production
- Plant biochemistry and biosynthesis
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
Colorado School of Mines
2010-2015
ABSTRACT The accumulation of bioenergy carriers was assessed in two starchless mutants Chlamydomonas reinhardtii (the sta6 [ADP-glucose pyrophosphorylase] and sta7 - 10 [isoamylase] mutants), a control strain (CC124), complemented strains the mutant. results indicate that genetic blockage starch synthesis increases lipids on cellular basis during nitrogen deprivation relative to CC124 as determined by conversion fatty acid methyl esters. However, this increased level lipid is energetically...
The plant terpenoids limonene (C10H16) and α-bisabolene (C15H24) are hydrocarbon precursors to a range of industrially relevant chemicals. High-titer microbial synthesis could pave the way for advances in vivo engineering tailor-made hydrocarbons, production at commercial scale. We have engineered fast-growing unicellular euryhaline cyanobacterium Synechococcus sp. PCC 7002 produce yields 4 mg L(-1) 0.6 through heterologous expression Mentha spicatal-limonene synthase or Abies grandis...
The cyanobacterium Synechococcus sp. PCC 7002 was genetically engineered to synthesize biofuel compatible medium-chain fatty acids during photoautotrophic growth. Expression of a heterologous lauroyl-acyl carrier protein (C12:0-ACP) thioesterase with concurrent deletion the endogenous putative acyl-ACP synthetase led secretion transesterifiable C12:0 acid in CO2-supplemented batch cultures. When grown at steady state over range light intensities an LED turbidostat photobioreactor,...
Photosynthetic microorganisms are able to produce a diverse array of renewable biochemical commodities. Although promising platforms for the accumulation targeted products, these organisms must be optimized in solar energy conversion, carbon capture and utilization, partitioning metabolic flux requisite biosynthetic pathways. Metabolic engineering efforts systematically addressing obstacles demonstrate potential develop consolidated bioprocessing that efficiently transform sunlight directly...
The defining challenge of energy research in the 21st century is development and deployment technologies for large-scale reconfiguration global infrastructure. Modern society built upon a concentrated yet finite reservoir diverse hydrocarbons formed through photosynthetic transformation several hundred million years solar energy. In human history, fossil era will be short lived never repeated. Although timing peak oil extensively debated, it an eventuality. It is, therefore, imperative that...