Calcineurin B-like (CBL)-interacting protein kinases (CIPKs) play a crucial role in the complex molecular systems of plants, acting as coordinators different plant development processes, manage hormone signaling, and respond to environmental stress. Despite their well-established importance various physiological process, CIPK gene family banana has remained an unexplored, creating knowledge gap that this research aims address. Through comprehensive analysis, we identified characterized 34...

ABSTRACT Freshwater scarcity and regulations on wastewater disposal have necessitated the reuse of treated (TWW) for soil irrigation, which has several environmental economic benefits. However, TWW irrigation can cause nutrient loading to receiving environments. We assessed bacterial community structure associated biogeochemical changes in plots irrigated with nitrate-rich (referred as pivots) periods ranging from 13 30 years. Soil cores (0 40 cm) were collected summer winter five pivots...

Despite the recent advancements in culturomics, isolation of majority environmental microbiota performing critical ecosystem services, such as bioremediation contaminants, remains elusive. Towards this end, we conducted a metagenomics-guided comparative assessment soil microbial diversity and functions present uraniferous soils relative to those that grew diffusion chambers (DC) or traps (MT), followed by uranium (U) resistant microbiota. Shotgun metagenomic analysis performed on used...

The majority of environmental microbiomes are not amenable to cultivation under standard laboratory growth conditions and hence remain uncharacterized. For applications, such as bioremediation, it is necessary isolate microbes performing the desired function, which may necessarily be fast growing or copiotroph microbiota. Toward this end, isolation microbial strains using diffusion chambers (DC) and/or traps (MT) have both been recently demonstrated effective strategies because enrichment...

Annona squamosa L., a popular fruit tree, is the most widely cultivated species of genus Annona. The lack transcriptomic and genomic information limits scope genome investigations in this important shrub. It bears aggregate fruits with numerous seeds. A few rare accessions very seeds have been reported for massive pyrosequencing (Roche, 454 GS FLX+) transcriptome from early stages development (0, 4, 8 12 days after pollination) was performed to produce expression datasets two genotypes,...

Nuclear production and industrial activities led to widespread contamination of the Department Energy managed Savannah River Site (SRS), located in South Carolina, USA. The H-02 wetland system was constructed 2007 for treatment storm water runoff from SRS Tritium Facility. Albeit at low levels, mercury (Hg) has been detected soils ecosystem. In anoxic sediments, Hg is typically methylated by anaerobic microbiota, forming highly neurotoxic methylmercury (MeHg), which biomagnifies across food...

Two Burkholderia spp. (strains SRS-25 and SRS-46) were isolated from high concentrations of uranium (U) the U.S. Department Energy (DOE)-managed Savannah River Site (SRS). SRS contains soil gradients that remain co-contaminated by heavy metals previous nuclear weapons production activities. Uranium is one dominant contaminants within impacted soils, which can be microbially transformed into less toxic forms. We established microcosms containing strains SRS-46 spiked with U evaluated...

Amplified fragment length polymorphism (AFLP) was used to assess genetic relatedness among 23 popular commercial cultivars of lychee ( Litchi chinensis ) and identify the markers associated with small-seeded cultivars. A combination three primer pairs high discriminatory ability selected for resolving identity The marker system resolved instances synonymies homonymies. two cultivars, Dehrarose Dehradun, whose names are often interchangeably, could be discriminated by AFLP. cultivar,...

The eastern oysters serve as important keystone species in the United States, especially Gulf of Mexico estuarine waters, and at same time, provide unparalleled economic, ecological, environmental, cultural services. One ecosystem service that has garnered recent attention is ability to sequester impurities nutrients, such nitrogen (N), from water feeds them, via their exceptional filtration mechanism coupled with microbially-mediated denitrification processes. It oyster-associated...

The soil-mousse surrounding a geothermal spring was analyzed for bacterial and archaeal diversity using 16S rRNA gene amplicon metagenomic sequencing which revealed the presence of 18 phyla distributed across 109 families 219 genera. Firmicutes, Actinobacteria, Deinococcus-Thermus group were predominant assemblages with Crenarchaeota Thaumarchaeota as main in this largely understudied habitat. Several metagenome sequences remained taxonomically unassigned suggesting repertoire hitherto...

Litchi chinensis is a subtropical fruit crop, popular for its nutritional value and taste. Fruits with small seed size thick aril are desirable in litchi. To gain molecular insight into gene expression that leads to the reduction of chinensis, transcriptomes two genetically closely related genotypes, contrasting were compared developing ovules. The cDNA library constructed from early developmental stages ovules (0, 6, 14 days after anthesis) bold- small-seeded litchi genotypes yielded...

Abstract Shellfish, such as the eastern oysters ( Crassostrea virginica ) are not only valued seafood but also for ecosystem services they provide, including improving water quality and reducing eutrophication. Excess N causes eutrophication, harmful algal blooms, fish kills overall decline of estuarine ecosystems resulting in economic losses. Oyster reefs sequester enhance denitrification processes, however, information on cycling oyster microbiome is scarce with most studies focusing...

The carriage of both, heavy metal and antibiotic resistance appears to be a common trait in bacterial communities native long-term contaminated habitats, including the Savannah River Site (SRS). There is widespread soil contamination at SRS; United States Department Energy (DOE) facility with from past industrial nuclear weapons production activities. To further evaluate genomic metabolic traits that underpin resistance, robust mercury (Hg) uranium (U)-resistant strain- SRS-8-S-2018, was...

A soilborne Stenotrophomonas sp. strain (MA5) that is resistant to mercury was isolated. draft genome sequence-based analysis revealed a suite of gene determinants resist and other heavy metals, multidrug efflux, stress response, membrane transport, these provide cues mechanisms underpin cellular survival in contaminated soil.

A mercury (Hg)-resistant Serratia sp. strain, SRS-8-S-2018, was isolated, followed by generation of its draft genome sequence, which indicated a genomic size 5,323,630 bp composed 5,261 coding sequences. suite functions in strain SRS-8-S-2018 identified, and these likely facilitate survival metalliferous soil habitat.

Abstract Transcriptome data are widely used for functional analysis of genes. De-novo assembly transcriptome gives a large number unigenes. A proportion them remain unannotated. Efficient computational methods required identifying genes and modeling those regulatory roles. Principal component (PCA) was in novel approach to shortlist genes, independently annotation genome expression data, taking seed development Arabidopsis thaliana as representative case. PCA applied published from four...

<ns4:p><ns4:bold>Background: </ns4:bold>Accurate assessment of the abundance and composition microbial assemblages in a complex environmental sample depends on successful lysis cells, for which bead-beating is typically used. In this study, we compared two commonly used bead beaters, FastPrep Bead Ruptor Elite, their ability to lyse eastern-oyster-associated bacterial communities over three different time points.</ns4:p><ns4:p> <ns4:bold>Methods: </ns4:bold>Genomic DNA was extracted from...