A5013752136- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Chemical Synthesis and Analysis
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- Pharmacogenetics and Drug Metabolism
- Genomics and Chromatin Dynamics
- Carbohydrate Chemistry and Synthesis
- Genomics, phytochemicals, and oxidative stress
- Biopolymer Synthesis and Applications
- Diabetes and associated disorders
- Cancer-related Molecular Pathways
- Bioactive Compounds and Antitumor Agents
- Immune Response and Inflammation
- Synthetic Organic Chemistry Methods
- Bioinformatics and Genomic Networks
- Estrogen and related hormone effects
- Prostate Cancer Treatment and Research
- Molecular Biology Techniques and Applications
- Biochemical and Molecular Research
- Antimicrobial Peptides and Activities
- Hepatitis B Virus Studies
Helios Klinikum Krefeld
2024
Esslingen University of Applied Sciences
2012-2023
Klinikum rechts der Isar
2021-2022
Technical University of Munich
2022
München Klinik
2022
IIT Research Institute
2006-2021
University of California, Santa Cruz
2014-2020
German Cancer Research Center
2005-2016
Heidelberg University
2005-2016
Charité - Universitätsmedizin Berlin
2011-2016
The branched DNA hybridization assay has been improved by the inclusion of novel nucleotides, isoC and isoG, in amplification sequences to prevent non-specific hybridization. isoC, isoG-containing have no detectable interaction with any natural sequence. control turn permits increased signal amplification. Addition a 14 site preamplifier was found increase signal/noise ratio 8-fold. A set 74 oligonucleotide probes designed consensus HIV POL detection limit this new amplifier approximately 50...
Journal Article A comparison of non-radioisotopic hybridization assay methods using fluorescent, chemiluminescent and enzyme labeled synthetic oligodeoxyribonucleotide probes Get access Mickey S. Urdea, Urdea Chiron Corporation4560 Horton Street, Emeryville, CA 94608, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Brian D. Warner, Warner Joyce A. Running, Running Michelle Stempien, Stempien Jennifer Clyne, Clyne Thomas Horn Nucleic Acids Research, Volume...
The design of RNA interference (RNAi) reagents is an essential step for performing loss-of-function studies in many experimental systems. availability sequenced and annotated genomes greatly facilitates RNAi experiments increasing number organisms that were previously not genetically tractable. E-RNAi web-service, accessible at http://www.e-rnai.org/, provides a computational resource the optimized evaluation reagents. 2010 update now covers 12 genomes, including Drosophila, Caenorhabditis...
Genetic screens for phenotypic similarity have made key contributions to associating genes with biological processes. With RNA interference (RNAi), highly parallel phenotyping of loss‐of‐function effects in cells has become feasible. One the current challenges however is computational categorization visual phenotypes and prediction function In this study, we describe a combined experimental approach discover novel gene functions explore functional relationships. We performed genome‐wide RNAi...
RNA interference (RNAi) represents a powerful method to systematically study loss-of-function phenotypes on large scale with wide variety of biological assays, constituting rich source for the assignment gene function. The GenomeRNAi database (http://www.genomernai.org) makes available RNAi phenotype data extracted from literature human and Drosophila. It also provides reagent information, along an assessment as their efficiency specificity. This manuscript describes update previously...
Gene–gene interactions shape complex phenotypes and modify the effects of mutations during development disease. The statistical gene–gene on have been used to assign genes functional modules. However, directional, epistatic interactions, which reflect regulatory relationships between genes, challenging map at large-scale. Here, we combinatorial RNA interference automated single-cell phenotyping generate a large genetic interaction for 21 phenotypic features Drosophila cells. We devised...
The synthesis of oligothymidilic acids, (dT)m (where m = 4, 7, 10, 13, 16, 19, 22, and 25), was carried out using a solid phase approach in combination with the modified phosphotriester methodology developed solution. Cellulose used as support after its functionalization specially featured dinucleoside diphosphate, 5'-0-p-chlorophenylphospho-2'(3')-0-acetyluridilyl-[2'(3')-3']-5'-0-dimethoxytritylthymidine p-chlorophenylester. fully protected trideoxynucleoside triphosphate containing only...
Significance Lipoxygenases are lipid-peroxidizing enzymes that have been classified according to their reaction specificity. ALOX15 (12/15-lipoxygenase) has implicated in inflammatory resolution via biosynthesis of antiinflammatory and proresolving lipoxins. We found lower mammals including primates express arachidonic acid 12-lipoxygenating orthologs, whereas higher 15-lipoxygenating enzymes. Gibbons constitute the missing link interconnecting 12- orthologs. To explore evolutionary driving...
Radiofrequency radiation (RFR) causes heating, which can lead to detrimental biological effects. To characterize the effects of RFR exposure on body temperature in relation animal size and pregnancy, a series short-term toxicity studies was conducted unique system. Young old B6C3F1 mice young, old, pregnant Harlan Sprague-Dawley rats were exposed Global System for Mobile Communication (GSM) or Code Division Multiple Access (CDMA) (rats = 900 MHz, 1,900 MHz) at specific absorption rates...
Antigen expression corresponding to anti-islet cell surface monoclonal antibodies IC2 and A2B5 was studied. is a rat-rat hybridoma autoantibody produced from the BB rat; among islet cells, β-cell specific. an anti-ganglioside antibody described as labeling β-cells. Islets of Langerhans Lewis rats were isolated cultured for 18 h in RPMI-1640 with five different glucose concentrations (2.2, 3.3, 5.5, 11.1, 18.3 mM). In some experiments, islets precultured 2 or 3 days. After isolation cells...
RNA interference (RNAi) has become a powerful genetic approach to systematically dissect gene function on genome-wide scale. Owing the penetrance and efficiency of RNAi in invertebrates, model organisms such as Drosophila melanogaster Caenorhabditis elegans have contributed significantly identification novel components diverse biological pathways, ranging from early development fat storage aging. For correct assessment phenotypes, key issue remains stringent quality control long...
Abstract RNA interference (RNAi) screens have enabled the systematic analysis of many biological processes in cultured cells and whole organisms. The success such interpretation data depend on stringent design RNAi libraries. We describe validate NEXT-RNAi, a software for automated evaluation sequences genome-wide scale. NEXT-RNAi is implemented as open-source accessible at http://www.nextrnai.org/.
Nucleoside phosphoramidite derivatives containing two protected primary hydroxyl functions have been incorporated into synthetic oligonucleotides as 'branching monomers'. With selective deprotection, multiple identical copies of an additional oligonucleotide can be to form fork- or comb-like structures for use signal amplification materials in nucleic acid hybridization assays.
Abstract Background Freshwater planarians are an attractive model for regeneration and stem cell research have become a promising tool in the field of regenerative medicine. With availability sequenced planarian genome, recent application modern genetic high-throughput tools has resulted revitalized interest these animals, long known their amazing capabilities, which enable them to regrow even new head after decapitation. However, detailed description transcriptome is essential future...
RNA interference (RNAi) has emerged as a powerful tool to generate loss-of-function phenotypes in variety of organisms. Combined with the sequence information almost completely annotated genomes, RNAi technologies have opened new avenues conduct systematic genetic screens for every gene genome. As increasing large datasets RNAi-induced become available, an important challenge remains integration and annotation functional information. Genome-wide been performed both Caenorhabditis elegans...
Like classical chemotherapy regimens used to treat cancer, targeted therapies will also rely upon polypharmacology, but tools are still lacking predict which combinations of molecularly drugs may be most efficacious. In this study, we image-based proliferation and apoptosis assays in colorectal cancer cell lines systematically investigate the efficacy two six that target critical oncogenic pathways. Drug pairs targeting key signaling pathways resulted synergies across a broad spectrum...